Neuroscience Newsletter - 13th Edition

Date: 
Monday, February 27, 2012 - 00:00
Summary: 
In this edition, RBFOX3/NeuN is spotlighted. You can also read about featured RBFOX3/NeuN Antibodies and related Neuroscience Antibodies.

 

Neuroscience Newsletter

In This Edition

Spotlight on RBFOX3/NeuN

NeuN (Neuronal Nuclei) is a neuron-specific protein which is present in most neuronal cell types of vertebrates and is used in research and diagnostics to identify postmitotic neurons. NeuN was first discovered in 1992 by R.J. Mullen et al. using specific monoclonal antibodies with brain cell nuclei antigens (PMID: 1483388). Subsequent studies revealed that anti-NeuN was highly specific for post mitotic neurons, stained most types of neurons in the nervous system, and reacted in multiple vertebrate species.

Anti-NeuN became a popular neuronal marker for research in neuroscience, developmental biology, neuronal stem cell differentiation, and also in histopathology for diagnosing neural diseases and tumors. However, despite widespread use of the NeuN antibody, the exact protein responsible for its specificity remained a mystery. In 2009, Kim et al. identified the responsible protein as RNA binding protein FOX3 (RBFOX3) (PMID: 19713214).

RBFOX3 is a member of the FOX-1 family, FOX proteins are believed to have a role in the regulation of neural specific mRNA splicing. There are three homologs of FOX-1; each has a central RNA recognition motif.

The discovery of NeuN as RBFOX3 significantly helped with this area of neuronal research. Today NeuN/RBFOX3 is still used as an important neuronal marker for neuroscience, developmental biology, neuronal stem cell research, and histopathology.

Featured RBFOX3/NeuN Antibodies

RBFOX3/NeuN (1B7) Antibody
Mouse Monoclonal
Catalog #: NBP1-92693
Sample Size Available
Reacts with: Bv, Hu, Mu, Po, and Rt
Works in: IF, WB

RBFOX3/NeuN Antibody
Rabbit Polyclonal
Catalog #: NBP1-92716
Sample Size Available
Reacts with: Bv, Hu, Mu, Po, Rt
Works in: IF, IHC-Fr, WB

RBFOX3/NeuN Antibody
Rabbit Polyclonal
Catalog #: NBP1-77686
Sample Size Available
Reacts with: Hu, Mu
Works in: ICC, IF, IHC-P

IF Image of RBFOX3
IF Image of RBFOX3
IHC Image of RBFOX3

Rat brain neural cultures stained with NBP1-92693 (red), a chicken polyclonal antibody to GFAP (NBP1-05198, green) and DNA (blue).

PFA fixed frozen section of rat cortex stained with
NBP1-92716 in red and counterstained for DNA in blue.

IHC analysis of RBFOX3/NeuN in mouse brain using DAB with hematoxylin counterstain.

References

  • Mullen RJ et al. “NeuN, a neuronal specific nuclear protein in vertebrates.” Development. 1992 Sep;116(1):201-11.
  • Kim KK et al. “Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors.”J Biol Chem. 2009 Nov 6;284(45):31052-61. Epub 2009 Aug 27.

We look forward to providing you with quality research tools,

Best regards,

Stephanie Peacock
Director of Marketing
Novus Biologicals
stephanie@novusbio.com
303.730.1905