DNA Repair eNews - 1st Edition

Spotlight on 53BP1
P53-binding protein 1 (53BP1) is involved in the early stages of cellular response to DNA double-strand breaks.  Upon DNA damage, 53BP1 is phosphorylated and relocalizes to the double-strand break sites. Methylation of histone H4 at Lys-20 is required for efficient localization to these sites.  53BP1 plays a redundant role in activation  of the downstream checkpoint effector proteins Brca1 and Chk2, but is required for the formation of Brca1 foci for the recruitment of repair/signaling proteins to sites of DNA damage. 53BP1 also plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Browse all 53BP1 antibodies.

NB100-304	NB100-904	NB100-305
53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours.	Western blot detection of 53BP1 (220 kDa) on untreated U2OS cell lysate using NB100-904.	Immunohistochemical analysis of placental villi using NB100-305.

Publications Citing NB100-304
Attwooll CL, Akpinar M, Petrini JH. The Mre11 Complex and the Response to Dysfunctional Telomeres. Mol Cell Biol 2009:29(20):5540-51. [PMID: 19667076]

Spardy N, Covella K, Cha E, et al. Human Papillomavirus 16 E7 Oncoprotein Attenuates DNA Damage Checkpoint Control by Increasing the Proteolytic Turnover of Claspin. Cancer Res 2009:69(17):7022-9. [PMID: 19706760]

Cadot B, Brunetti M, Coppari S, et al. Loss of Histone Deacetylase 4 Causes Segregation Defects during Mitosis of p53-Deficient Human Tumor Cells. Cancer Res 2009;69(15):6074-6082. [PMID: 19622775]

Coming Soon: Customer Reviews Published on our Website

53BP1 Antibody [NB100-304]

This antibody was given four out of five stars in a customer review submitted on May 27th, 2009.  The customer used the antibody for Immunofluorescent staining of mouse B cells at a 1:500 dilution.   The cells were first fixed with Formaldehyde and permeabilized using Triton-X100.  The 53BP1 antibody was incubated for one hour at room temperature in PBS + 0.2% BSA + 0.1% Tween 20.  The secondary antibody used was an Alexa fluor 633 goat anti-rabbit used at a dilution of 1:500, and was incubated at room temperature for one hour in PBS + 0.2% BSA + 0.1% Tween 20.

New Product Information
•25% OFF select DNA Repair-related antibodies and proteins. View the list here.