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DNA Newsletter- 11th Edition

DNA Newsletter- 11th Edition October 27, 2011

Summary: 
In this edition of the DNA Newsletter, Direct Reversal Repair is spotlighted. Also read about Direct Reversal Repair in the news and learn more about MGMT, ABH2, and ALKBH3.
Date: 
October 27, 2011

 

DNA Newsletter

Edition Contents

Spotlight on Direct Reversal Repair

Direct Reversal Repair (DRR) is the simplest form of DNA damage repair, consisting of direct chemical reversal of the reaction that caused damage. Limited to single base damage, DRR mechanisms are highly specific for each damage type and do not require breaking the phosphodiesterDNA backbone.

One type of DRR involves the demethylation of guanine bases by methyl guanine methyl transferase (MGMT). In this process, MGMT accepts the DNA adduct in a non-reversible stoichiometric reaction.Another involves repair against the cytotoxicity of methylating agents by ABH2 and ALKBH3. These two proteins catalyze the removal of 1-methyladenine and 3-methylcytosine to repair the specific DNA lesions generated in single-stranded DNA. Together, these two DRR mechanisms compromise a cell’s first line of defense against simple forms of DNA damage.

Novus offers top quality research antibodies for all three of these DRR proteins, with various testing data highlighted below.

DRR Antibodies

IF Image of MGMT

MGMT stain in Hela cells using MGMT antibody [NB100-168] with a FITC secondary (green). Nuclei (blue) are counterstained using Hoechst 33258.

 

ALKBH2 in human kidney
tissue lysate using ALKBH2 antibody [NBP1-76276] at
(A) 1 and (B) 2ug/mL.

Immunohistochemistry: MGMT Antibody (MT 3.1) [NB 100-692] – MGMT stain in FFPE human colon carcinoma stained with using peroxidase-conjugate and AEC chromogen.

ALKBH3 in Fetal Muscle tissue lysate using ALKBH3 antibody [NBP1-57586] at 1ug/ml.

In the News

  1. Loh YH, Mitrou PN, Wood A, et al. SMAD7 and MGMT genotype variants and cancer incidence in the European Prospective Investigation into Cancer and Nutrition (EPIC)-Norfolk Study.Cancer Epidemiol. 2011 Aug;35(4):369-74. [PMID:21075068]

  2. Giri NC, Sun H, Chen H, et al. X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2.Biochemistry. 2011 Jun 7; 50(22):5067-76. [PMID:21510633]

  3. Tasaki M, Shimada K, Kimura H, et al. ALKBH3, a human AlkB homologue, contributes to cell survival in human non-small-cell lung cancer.Br J Cancer. 2011 Feb 15;104(4):700-6. [PMID:21285982]

Novus also offers many other quality MGMT, AGH2 and ALKBH3 reagents. Please contact us at technical@novusbio.com if you have any questions or would like help finding the best reagent for your experiment.

We look forward to providing you with quality research tools,

Amy Chamberlain-Torres
Product Marketing Manager
stephanie@novusbio.com