Cancer Research eNews - 1st Edition

Spotlight on Beclin 1
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that Beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections.

NB110-87318
Detection of Beclin 1 in HeLa whole cell lysate using NB110-87318 at 1:10,000.	Staining of normal breast tissue using NB110-87318 (20x).

Beclin Publications
Pattingre S, Bauvy C, Carpentier S, et al. Role of JNK1-dependent Bcl-2 Phosphorylation in Ceramide-induced Macroautophagy. J Biol Chem 2009;284(5):2719-2728. [PMID: 19029119]

Bellot G, Garcia-Medina R, Gounon P, et al. Hypoxia-Induced Autophagy Is Mediated through Hypoxia-Inducible Factor Induction of BNIP3 and BNIP3L via Their BH3 Domains. Mol Cell Biol 2009;29(10):2570-2581. [PMID: 19273585]

Kyei GB, Dinkins C, Davis AS, et al. Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages. J Cell Biol 2009;186(2):255-268. [PMID: 19635843]

Hoang B, Benavides A, Shi Y, et al. Effect of autophagy on multiple myeloma cell viability. Mol Cancer Ther 2009;8(7):1974-1984. [PMID: 19509276]

Coming Soon: Customer Reviews Published on our Website

GAPDH Antibody [NB300-221]

This antibody was given 5 stars in a customer review submitted June 4, 2009. The customer used the antibody in Western Blot on EAhy926 cell lysate at a dilution of 1:5,000.   A band was visualized at 30-40 kDa.  The customer praised the specificity of this antibody saying: “We’ve been using this product for many years.”

NOX4 Antibody [NB110-58851]

This antibody was given 4 stars in a customer review submitted March 26, 2009.  The customer used the antibody in Western Blot on rat liver whole homogenate and primary hepatocyte cell lysate and detected bands at 31 kDa and 67 kDa.

  Reviewed by Matt Morris (emmgw7@mail.mizzou.edu)

New Product Information
•25% OFF select cancer-related antibodies and proteins. View the list here.

•Carbonic Anhydrase IX is now available conjugated to Dylight 488™, DyLight 549™   and DyLight 649™. View the available Carbonic Anhydrase IX products here.

Publications
[NB400-105 ABCA1] Sirek AS, Liu L, Naples M, et al. Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, and Unc-86 Homeodomain Protein Octamer Transcription Factor-1. Endocrinology 2009;150(8):3483-3492. [PMID: 19359385]

[NB400-148 Niemann-Pick C1] Rocha N, Kuijl C, van der Kant R, et al. Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150Glued and late endosome positioning. J Cell Biol 2009;185(7):1209-1225. [PMID: 19564404]

[NB110-58849 NOX4] Eid AA, Gorin Y, Fagg BM, et al. Mechanisms of Podocyte Injury in Diabetes: Role of Cytochrome P450 and NADPH Oxidases. Diabetes 2009;58(5):1201-1211. [PMID: 19208908]