XAGE1D Antibody 0.1 mg

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Immunohistochemistry-Paraffin: XAGE1D Antibody [NB300-997] - (3.8ug/ml) IHC staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Ordering Information: NB300-997

  • Catalog Number
    NB300-997
  • Also Known As
    Imgenex: IMG-3758
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 mg
  • Availability
  • Price
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XAGE1D Antibody Summary

Species Human
Tested Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Gene
XAGE1D
Purity
Immunogen affinity purified
Specificity
This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
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XAGE1D Antibody Details

Immunogen
Synthetic peptide CGFGFRRQGEDNT, representing the C-Terminus of the human protein sequence according to NP_597673.

Species Reactivity

Human

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-6 ug/ml
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA detection limit 1:8000

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Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-saline (pH7.3) with 0.5% BSA
Unit Size
0.1 mg
Concentration
0.5 mg/ml
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol XAGE1D
Entrez
Uniprot

Alternate Names for XAGE1D Antibody

  • cancer/testis associated protein
  • CT12.1C
  • CTP9
  • G antigen family D member 2
  • member 1c
  • X antigen family, member 1C
  • XAGE1
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Background

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene generates 3 transcript variants, and one of which includes 2 transcripts generated from alternate transcription initiation sites.

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Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

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FAQs for XAGE1D (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for XAGE1D

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