Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Human recombinant WT1 protein (residues 1-181). [Swiss-Prot# P19544]
Localization:
Nuclear
Epitope:
Residues 1-84.
Species Reactivity:
This antibody reacts with human. It is expected to react with mouse protein, as well. Other species have not been tested.
Applications:
Uses:
This antibody is useful for Western blot analysis where a doublet is seen ~55 kDa. Previous productions have been useful for immunoprecipitation and immunohistochemistry, as well.
Dilutions:
immunohistochemistry ,
immunoprecipitation ,
Western Blot 2 ug/ml
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms' tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.