Wilms Tumor 1 Antibody (NB110-60011R)

Wilms Tumor 1 Antibody (6F-H2) [DyLight 550]

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Wilms Tumor 1 Antibody (6F-H2) [DyLight 550] Summary:
Species:Hu
Applications:IHC, IP, WB
Clonality:Monoclonal
Gene:WT1
Purity:IgG purified
Host:Mouse
Specificity:Wilms Tumor 1 (6F-H2)
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Wilms Tumor 1 Antibody (6F-H2) [DyLight 550] Details:
Conjugate:DyLight 550
Clone:6F-H2
Isotype:IgG1 Kappa
Immunogen:Human recombinant WT1 protein (residues 1-181). [Swiss-Prot# P19544]
Localization:Nuclear
Epitope:Residues 1-84.
Species Reactivity:

This antibody reacts with human. It is expected to react with mouse protein, as well. Other species have not been tested.

Applications:
Uses:This antibody is useful for Western blot analysis where a doublet is seen ~55 kDa. Previous productions have been useful for immunoprecipitation and immunohistochemistry, as well.
Dilutions:immunohistochemistry , immunoprecipitation , Western Blot 2 ug/ml
Unit Size:0.1 ml
Concentration:0.8 mg/ml
Positive Controls:1 Positive Controls
Conjugated Antibodies:4 Conjugated Antibodies
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:50mM sodium borate
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: WT1
Entrez7490 (Human)
Swiss ProtP19544 (Human)
 
Background:

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms' tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.

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Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Conjugated Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Wilms Tumor 1 Antibody (6F-H2) [DyLight 488]NB110-60011GHuIHC, IP, WB

Wilms Tumor 1 Antibody (6F-H2) [DyLight 650]NB110-60011CHuIHC, IP, WB

Wilms Tumor 1 Antibody (6F-H2) [Biotin]NB110-60011BHuIHC, IP, WB

Wilms Tumor 1 Antibody (6F-H2) [HRP]NB110-60011HHuIHC, IP, WB

Positive Controls
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Other Positive Controls
  • 293 and K562 cell lysates
Lysates (2)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Wilms Tumor 1 LysateNBL1-17887WB(1)

Wilms Tumor 1 LysateH00007490-T02WB

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Wilms Tumor 1 PeptideNB100-41383PEPIF, PEP-ELISA

Wilms Tumor 1 Recombinant ProteinH00007490-P01HuELISA, WB(1)

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Primary Antibodies (21)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Wilms Tumor 1 Antibody (6F-H2)NB110-60011HuIHC-P, IP, WB(2)(1)

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RNAi (4)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Wilms Tumor 1 RNAiH00007490-R02Hu(1)(4)

Wilms Tumor 1 RNAiH00007490-R04Hu(1)(4)

Wilms Tumor 1 RNAiH00007490-R01Hu(1)(4)

Wilms Tumor 1 RNAiH00007490-R03Hu(1)(4)

Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Mouse IgG Antibody [Biotin]NB720-BMuELISA, IHC-P, WB

Mouse IgG Antibody [HRP]NB720-HMuELISA, ICC, IHC-P, WB

Mouse IgG Antibody [Alkaline Phosphatase]NB720-APMuELISA, ICC, IHC-P, WB

Mouse IgG Antibody [FITC]NB720-FMuICC, IHC-P(2)

Mouse IgG AntibodyNB120-7056Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)ELISA, IHC-P, IP, WB

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Wilms Tumor 1 in Context


Research Areas related to Wilms Tumor 1 (6):
Novus Explorer for Wilms Tumor 1 gene

Try our bioinformatic tool that shows the relationships between WT1 and other genes, diseases, pathways and post-translational modifications.