Wilms Tumor 1 Antibody (6F-H2) (NB110-60011)

Wilms Tumor 1 Antibody (6F-H2) (NB110-60011) (0.1 ml)

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	Catalog Number	NB110-60011 (Sample Size Available)
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anti-Wilms tumor protein antibody, anti-WIT-2 antibody, anti-WAGR antibody, anti-AWT1 antibody, anti-NPHS4GUD antibody, anti-WT33 antibody, anti-Wilms tumor 1 antibody

Wilms Tumor 1 Antibody (6F-H2) Summary:

Species:	Hu	Gene:	WT1
Applications:	WB, IHC-P, IP	Purity:	IgG purified
Clonality:	Monoclonal	Host:	Mouse
Specificity:	Wilms Tumor 1 (6F-H2)
Publications:	NB110-60011 has been mentioned in at least 2 Publications

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Wilms Tumor 1 Antibody (6F-H2) Details:

Clone:	6F-H2
Isotype:	IgG1 Kappa
Immunogen:	Human recombinant WT1 protein (residues 1-181). [Swiss-Prot# P19544]
Localization:	Nuclear
Epitope:	Residues 1-84.

Species Reactivity:

This antibody reacts with human. It is expected to react with mouse protein, as well. Other species have not been tested.

Applications:

Uses:	This antibody is useful for Western blot analysis where a doublet is seen ~55 kDa and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Previous productions have been useful for immunoprecipitation, as well.
Dilutions:	Immunohistochemistry-Paraffin 1:400, Immunoprecipitation, Western Blot 2 ug/ml
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	0.8 mg/ml
Positive Controls:	5 Positive Controls
Conjugated Antibodies:	5 Conjugated Antibodies

Packaging:

Storage:	Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer:	Tris-glycine, 150mM NaCl
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: WT1

Entrez:	7490 (Human)
Uniprot:	P19544 (Human)

Related Genes:

Background:

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms' tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.

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