VMAT2 Antibody 0.025 ml

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Western Blot: VMAT2 Antibody [NBP1-69750] - WB analysis of VMAT2 in rat striatal tissue lysate. Image courtesy of anonymous customer product review.
Immunohistochemistry: VMAT2 Antibody [NBP1-69750] - IHC staining of VMAT2 in mouse brain cortex.
Western Blot: VMAT2 Antibody [NBP1-69750] - Analysis of VMAT2 in human brain lysate.
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Ordering Information: NBP1-69750

  • Catalog Number
    NBP1-69750
  • Displayed Format
    Unconjugated
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VMAT2 Antibody Summary

Species Human, Mouse, Rat
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
SLC18A2
Purity
Immunogen affinity purified
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VMAT2 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human VMAT2 protein (within residues 30-200). [Swiss-Prot Q05940]
Localization
Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Species Reactivity

Human, rat and mouse.
Reviewed Species Read 1 rated 4
using NBP1-69750 in the following species:

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This VMAT2 antibody is useful for IHC and Western blot where a doublet is seen ~60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-69750AF405
Alexa Fluor (R) 488 Labeled NBP1-69750AF488
Alexa Fluor (R) 647 Labeled NBP1-69750AF647
Alexa Fluor (R) 700 Labeled NBP1-69750AF700
Biotin Labeled NBP1-69750B
DyLight 405 Labeled NBP1-69750V
DyLight 488 Labeled NBP1-69750G
DyLight 550 Labeled NBP1-69750R
DyLight 650 Labeled NBP1-69750C
FITC Labeled NBP1-69750F
HRP Labeled NBP1-69750H
Reviewed Applications
Read 1 rated 4
using
NBP1-69750 in the following applications:

Publications
Read a Publication using
NBP1-69750 in the following applications:

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    Product PDFs

    Frequently Purchased Together

    Reviews

    Average Rating: 4
    Recent Reviews
    WB Tested In Rat
    Reviewed On: 09/15/2011
    Rated: 4

    VMAT2 NBP1-69750
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    Publications

    VMAT2 Antibody (NBP1-69750) has been mentioned in at least 1 publication.
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    Notes

    Manufactured by Genomic Antibody Technology™. GAT FAQs

    Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS, 30% glycerol
    Unit Size
    0.025 ml
    Concentration
    1.36 mg/ml
    Preservative
    0.05% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol SLC18A2
    Entrez
    Uniprot

    Alternate Names for VMAT2 Antibody

    • MGC120477
    • monoamine neurotransmitter transporter
    • Monoamine transporter
    • solute carrier family 18 (vesicular monoamine), member 2
    • Solute carrier family 18 member 2
    • SVAT
    • SVMTMGC120478
    • synaptic vesicular amine transporter
    • VAT2MGC26538
    • vesicle monoamine transporter type 2
    • vesicle monoamine/H+ antiporter
    • Vesicular amine transporter 2
    • VMAT2
    Show More

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    Reviews for VMAT2 Antibody (NBP1-69750) (1) 41

    Read what people are saying who have used VMAT2 Antibody (NBP1-69750).

    We have 1 review tested in 1 species: Rat.
    We have 1 review tested in 1: Western Blot.
    Average Rating: 4
    (Based on 1 reviews)

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    Sort 1 reviews for VMAT2 Antibody (NBP1-69750) by:
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    Images Ratings Applications Species Reviewed By Date Details
    Western Blot VMAT2 NBP1-69750
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    Very Good
    WB Rat Anonymous 09/15/2011
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    Summary

    ApplicationWestern Blot
    Sample TestedE13 rat RN46A-B14 cells from medullary raphe, Sample Amount: 10ug rat striatal vesicular tissue, 25 ug cell vesicular fraction
    Sample Pretreatmentsubcellular fractions used with specific protocol
    SpeciesRat
    LotA
    CommentsWe give the VMAT2 antibody 4 stars. It worked very well for us in rat brain tissue.

    Blocking

    Blocking DetailsBlocking Buffer: 0.2% TBS-T with 5% milk

    Primary Anitbody

    Dilution RatioDilution Ratio: 1:5,000, Incubation Dilution Buffer: 0.2% TBS-T w/ 5% milk, Incubation Time: overnight, Incubation Temp: 4C

    Secondary Antibody

    Secondary Manufacturer Cat#Chemicon

    Details

    Detection NotesHyGlow HRP Detection, Pos Ctrl: Saline treated rat striatum, vesicular fraction preperation, Specific Bands: 60 kDa

    Publications for VMAT2 Antibody (NBP1-69750) (1)

    Read what people are writing who have used VMAT2 Antibody (NBP1-69750).

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    Publication Applications Species

    Product General Protocols

    View specific protocols for VMAT2 Antibody (NBP1-69750): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol
    ICC/IF Video Protocol

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    FAQs for VMAT2 (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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