VMAT2 Antibody Summary
Human, Mouse, Rat
WB, ICC/IF, IHC, IHC-P
Immunogen affinity purified
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VMAT2 Antibody Details
A genomic peptide made to an internal region of the human VMAT2 protein (within residues 30-200). [Swiss-Prot Q05940]
Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Human, rat and mouse.
1 rated 4
using NBP1-69750 in the following species:
Publications using NBP1-69750 in the following species:
Western Blot 1:10000 Immunocytochemistry/Immunofluorescence 1:10-1:500 Immunohistochemistry 1:100 Immunohistochemistry-Paraffin 1:100
This VMAT2 antibody is useful for IHC and Western blot where a doublet is seen ~60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Other Available Formats
1 rated 4
NBP1-69750 in the following applications:
Frequently Purchased Together
WB Tested In Rat
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Manufactured by Genomic Antibody Technology™. GAT
FAQs Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
Alternate Names for VMAT2 Antibody
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VMAT2 Antibody (NBP1-69750) (1)
Read what people are saying who have used VMAT2 Antibody (NBP1-69750).
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1: Western Blot.
Average Rating: 4
(Based on 1 reviews)
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Application Western Blot Sample Tested E13 rat RN46A-B14 cells from medullary raphe, Sample Amount: 10ug rat striatal vesicular tissue, 25 ug cell vesicular fraction Sample Pretreatment subcellular fractions used with specific protocol Species Rat Lot A Comments We give the VMAT2 antibody 4 stars. It worked very well for us in rat brain tissue. Blocking
Blocking Details Blocking Buffer: 0.2% TBS-T with 5% milk Primary Anitbody
Dilution Ratio Dilution Ratio: 1:5,000, Incubation Dilution Buffer: 0.2% TBS-T w/ 5% milk, Incubation Time: overnight, Incubation Temp: 4C Secondary Antibody
Secondary Manufacturer Cat# Chemicon Details
Detection Notes HyGlow HRP Detection, Pos Ctrl: Saline treated rat striatum, vesicular fraction preperation, Specific Bands: 60 kDa
Publications for VMAT2 Antibody (NBP1-69750) (1)
Read what people are writing who have used VMAT2 Antibody (NBP1-69750).
We have publications tested in 1 confirmed species: Mouse. We have publications tested in 1 applications: IHC-Fr.
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Product General Protocols
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FAQs for VMAT2 (2)
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit:
IHC-P Protocol and IHC-P Troubleshooting. I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for VMAT2
Discover related pathways, diseases and genes to VMAT2. Need help? Read the
Bioinformatics Tool Guide
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