VMAT2 Antibody 0.025 ml

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Western Blot: VMAT2 Antibody [NBP1-69750] - WB analysis of VMAT2 in rat striatal tissue lysate. Image courtesy of anonymous customer product review.
Immunohistochemistry: VMAT2 Antibody [NBP1-69750] - IHC staining of VMAT2 in mouse brain cortex.
Western Blot: VMAT2 Antibody [NBP1-69750] - Analysis of VMAT2 in human brain lysate.
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Ordering Information: NBP1-69750

  • Catalog Number
    NBP1-69750
  • Displayed Format
    Unconjugated
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VMAT2 Antibody Summary

Species Human, Mouse, Rat
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
SLC18A2
Purity
Immunogen affinity purified
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VMAT2 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human VMAT2 protein (within residues 30-200). [Swiss-Prot Q05940]
Localization
Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Species Reactivity

Human, rat and mouse.
Reviewed Species Read 1 rated 4
using NBP1-69750 in the following species:

Publications Read Publications using NBP1-69750 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This VMAT2 antibody is useful for IHC and Western blot where a doublet is seen ~60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-69750AF405
Alexa Fluor (R) 488 Labeled NBP1-69750AF488
Alexa Fluor (R) 647 Labeled NBP1-69750AF647
Alexa Fluor (R) 700 Labeled NBP1-69750AF700
Biotin Labeled NBP1-69750B
DyLight 405 Labeled NBP1-69750V
DyLight 488 Labeled NBP1-69750G
DyLight 550 Labeled NBP1-69750R
DyLight 650 Labeled NBP1-69750C
FITC Labeled NBP1-69750F
HRP Labeled NBP1-69750H
Reviewed Applications
Read 1 rated 4
using
NBP1-69750 in the following applications:

Publications
Read a Publication using
NBP1-69750 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 4
Recent Reviews
WB Tested In Rat
Reviewed On: 09/15/2011
Rated: 4

VMAT2 NBP1-69750
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Publications

VMAT2 Antibody (NBP1-69750) has been mentioned in at least 1 publication.
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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
1.36 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol SLC18A2
Entrez
Uniprot

Alternate Names for VMAT2 Antibody

  • MNAT
  • SLC18A2
  • SVAT
  • SVMT
  • VAT2
  • MGC120477
  • monoamine neurotransmitter transporter
  • Monoamine transporter
  • solute carrier family 18 (vesicular monoamine), member 2
  • Solute carrier family 18 member 2
  • SVAT
  • SVMTMGC120478
  • synaptic vesicular amine transporter
  • VAT2MGC26538
  • vesicle monoamine transporter type 2
  • vesicle monoamine/H+ antiporter
  • Vesicular amine transporter 2
  • VMAT2
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Related Products by Gene


Reviews for VMAT2 Antibody (NBP1-69750) (1) 41

Read what people are saying who have used VMAT2 Antibody (NBP1-69750).

We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1: Western Blot.
Average Rating: 4
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot VMAT2 NBP1-69750
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Very Good
WB Rat Anonymous 09/15/2011
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Summary

ApplicationWestern Blot
Sample TestedE13 rat RN46A-B14 cells from medullary raphe, Sample Amount: 10ug rat striatal vesicular tissue, 25 ug cell vesicular fraction
Sample Pretreatmentsubcellular fractions used with specific protocol
SpeciesRat
LotA
CommentsWe give the VMAT2 antibody 4 stars. It worked very well for us in rat brain tissue.

Blocking

Blocking DetailsBlocking Buffer: 0.2% TBS-T with 5% milk

Primary Anitbody

Dilution RatioDilution Ratio: 1:5,000, Incubation Dilution Buffer: 0.2% TBS-T w/ 5% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary Manufacturer Cat#Chemicon

Details

Detection NotesHyGlow HRP Detection, Pos Ctrl: Saline treated rat striatum, vesicular fraction preperation, Specific Bands: 60 kDa

Publications for VMAT2 Antibody (NBP1-69750) (1)

Read what people are writing who have used VMAT2 Antibody (NBP1-69750).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 applications: IHC-Fr.

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IHC-Fr
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Mouse
(1)
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Product General Protocols

View specific protocols for VMAT2 Antibody (NBP1-69750): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for VMAT2 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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