VEGF Antibody (VG76e) 0.1 ml

VEGF NB100-648

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Ordering Information: NB100-648

Catalog NumberNB100-648
  • FormUnconjugated
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VEGF Antibody (VG76e) Summary

 
Species Human, Bovine, Porcine, Sheep
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, RIA
Clonality Monoclonal
Host Mouse
Gene VEGFA
Purity Protein G purified
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VEGF Antibody (VG76e) Details

 
Clone VG76e
Isotype IgG1
Immunogen Recombinant human VEGF189 expressed in E.coli [UniProt# P15692]
Localization This antibody is highly specific, but only weakly stains transfected cells (5-10%).

Species Reactivity

 
Human. Mixed results in Cow and Pig (IHC only). Customers have reported success staining sheep tissue (see review).
Reviewed Species Read 3 rated 4  using NB100-648 in the following species:
 
Publications Read Publications using NB100-648 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 0.5-2ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
Application Notes

This VEGF antibody is useful for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Radioimmunoassay was reported in scientific literature. In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.

Molecular Weight

40 kDa

Positive Controls

2 Positive Controls

Other Available
Formats
7C Labeled NB100-648V2
Alexa Fluor (R) 405 Labeled NB100-648AF405
Alexa Fluor (R) 488 Labeled NB100-648AF488
Alexa Fluor (R) 647 Labeled NB100-648AF647
Alexa Fluor (R) 700 Labeled NB100-648AF700
Allophycocyanin Labeled NB100-648APC
Biotin Labeled NB100-648B
DyLight 350 Labeled NB100-648UV
DyLight 405 Labeled NB100-648V
DyLight 405LS Labeled NB100-648V3
DyLight 488 Labeled NB100-648G
DyLight 550 Labeled NB100-648R
DyLight 650 Labeled NB100-648C
DyLight 680 Labeled NB100-648FR
DyLight 755 Labeled NB100-648IR
FITC Labeled NB100-648F
HRP Labeled NB100-648H
PE Labeled NB100-648PE
PerCP Labeled NB100-648PCP
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 4  using
NB100-648 in the following application:
Publications
Read publications using
NB100-648 in the following applications:
  • WB2publications
  • IHC1publications
  • RIA1publications

Product PDFs

Reviews

Average Rating: 4 
Recent Reviews

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Publications

VEGF Antibody (NB100-648) has been mentioned in at least 7 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all VEGF Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer PBS
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1 mg/ml
Preservative No Preservative
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol VEGFA
Entrez
Uniprot

Alternate Names for VEGF

 
  • Vascular permeability factor
  • VEGF-A
  • VEGFMGC70609
  • VPFvascular endothelial growth factor
  • MVCD1
  • vascular endothelial growth factor A

Related Products by Gene

Background

 
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.