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VEGF Antibody (VG76e) (NB100-648) (0.1 ml)

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Catalog NumberNB100-648 ( Sample Size Available )
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View All VEGF Primary Antibodies
(see alternate names)
anti-Vascular permeability factor antibody, anti-VEGF-A antibody, anti-VEGFMGC70609 antibody, anti-VPFvascular endothelial growth factor antibody, anti-MVCD1 antibody, anti-vascular endothelial growth factor A antibody
VEGF Antibody (VG76e) Summary:
Species: Hu, Bv, Po, Sh Gene: VEGFA
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP Purity: Protein G purified
Clonality: Monoclonal Host: Mouse
Publications: NB100-648 has been mentioned in at least 6 Publications
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VEGF Antibody (VG76e) Details:
Clone: VG76e
Isotype: IgG1
Immunogen: Human VEGF189 expressed in E.coli [UniProt# P15692]
Localization: This antibody is highly specific, but only weakly stains transfected cells (5-10%).
  Species Reactivity:
Human. Mixed results in Cow and Pig (IHC only). Customers have reported success staining sheep tissue (see review).
  Applications:
Uses: This VEGF antibody is useful for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry (Formalin-fixed paraffin-embedded sections). In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.
Dilutions: ELISA 1:100-1:2000
Immunocytochemistry/Immunofluorescence 1:50-1:200
Immunohistochemistry 1:200
Immunohistochemistry-Paraffin 1:200
Immunoprecipitation 1:10-1:500
Western Blot 0.5-2ug/ml
MW : 40 kDa
Unit Size: 0.1 ml (also available: 0.025 ml (sample size) )
Concentration: 1 mg/ml
Positive Controls: 2 Positive Controls
Conjugated Antibodies: 5 Conjugated Antibodies
Packaging:
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer: PBS
Preservative: No Preservative
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
 
Gene Symbol: VEGFA
Entrez 7422 (Human)
Uniprot P15692 (Human)
Related Genes
Background:

VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.

Images (2)
Western Blot: VEGF Antibody (VG76e) [NB100-648] - Analysis of VEGF in human kidney protein (30ug). Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.
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Reviews (1)
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Anonymous on 5/4/2011
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Related Products (651)
2 Positive Controls
5 Conjugated Antibodies
2 ELISA Kits
6 Peptides and Proteins
19 Primary Antibodies
Secondary Antibodies
 
Exploring VEGF
5   Related Research Areas
 
Working with VEGF
6   Publications
1   Reviews from researchers like you
Jump to: Publications, Images, Reviews, Ask a Question
Working with VEGF  

VEGF Antibody (VG76e) Images (2)

Western Blot: VEGF Antibody (VG76e) [NB100-648] - Analysis of VEGF in human kidney protein (30ug).
 

(view larger) 		Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.
 
 

Reviews (1)  Have you used this product? Review this product to earn Novus Rewards.

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Reviewed by:
Application: IHC-P
Sample Tested: Fetal Sheep Brain Tissue
Sample Pretreatment: Hypoxia
Species: Sheep
Other Species: Spiny Mouse
Lot: Novus anti-VEGF[VG76e] lot 467/04
Blocking Details: Normal Goat Serum Block Buffer
Dilution Ratio: 1:200
Secondary Description: Rabbit anti-Mouse
Secondary Manufacturer Cat#: Vector
 

Publications (6)

 
VEGF Antibody (VG76e) Product Specific:
  1. Yu R, Dhall D, Nissen NN, et al. Pancreatic neuroendocrine tumors in glucagon receptor-deficient mice. PLoS ONE 20116(8). [PMID: 21853126]
  2. Mannheim D, et al. Impaired myocardial perfusion reserve in experimental hypercholesterolemia is independent of myocardial neovascularization. Am J Physiol Heart Circ Physiol;2925:H2449-58. 2007 May. [PMID: 17208989]

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  1. Fraser HM, Dickson SE, Lunn SF, Wulff C, Morris KD, Carroll VA, Bicknell R. Suppression of luteal angiogenesis in the primate after neutralization of vascular endothelial growth factor. Endocrinology;1413:995-1000. 2000 Mar. [PMID: 10698175]
  2. Zhang L, et al. Validation of anti-vascular endothelial growth factor anti-VEGF antibodies for immunohistochemical localization of VEGF in tissue sections: expression of VEGF in the human endometrium. J Pathol;1854:402-8. 1998 Aug. [PMID: 9828839]
  3. Brown NS, Streeter EH, Jones A, Harris AL, Bicknell R. Cooperative stimulation of vascular endothelial growth factor expression by hypoxia and reactive oxygen species: the effect of targeting vascular endothelial growth factor and oxidative stress in an orthotopic xenograft model of bladder carcinoma. Br J Cancer;929:1696-701. 2005 May 9. [PMID: 15841086]
  4. Collingridge DR, Carroll VA, Glaser M, Aboagye EO, Osman S, Hutchinson OC, Barthel H, Luthra SK, Brady F, Bicknell R, Price P, Harris AL. The development of [124I]iodinated-VG76e: a novel tracer for imaging vascular endothelial growth factor in vivo using positron emission tomography. . Cancer Res. ;6220:5912-9. . 2002 Oct 15. [PMID: 12384557]

Protocols

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Jump to: ELISA Kits, Conjugated Antibodies, Positive Controls, Peptides and Proteins, Primary Antibodies, Secondary Antibodies
  ELISA Kits (2)
Name Catalog# Reactivity Applications Images Publications Reviews
VEGF ELISA Kit [HRP] KA0650 Hu 0 0 0

VEGF ELISA Kit [Biotin] KA2201 Mu 0 0 0

  Conjugated Antibodies (5)
Name Catalog# Reactivity Applications Images Publications Reviews
VEGF Antibody (VG76e) [DyLight 550] NB100-648R Hu, Bv, Po, Sh WB, ELISA, ICC/IF, IHC, IHC-P, IP 0 0 0

VEGF Antibody (VG76e) [HRP] NB100-648H Hu, Bv, Po, Sh WB, ELISA, ICC/IF, IHC, IHC-P, IP 0 0 0

VEGF Antibody (VG76e) [DyLight 488] NB100-648G Hu, Bv, Po, Sh WB, ELISA, ICC/IF, IHC, IHC-P, IP 0 0 0

VEGF Antibody (VG76e) [DyLight 650] NB100-648C Hu, Bv, Po, Sh WB, ELISA, ICC/IF, IHC, IHC-P, IP 0 0 0

VEGF Antibody (VG76e) [Biotin] NB100-648B Hu, Bv, Po, Sh WB, ELISA, ICC/IF, IHC, IHC-P, IP 0 0 0

  Positive Controls (2)
Name Catalog# Reactivity Applications Images Publications Reviews
VEGFA Overexpression Lysate NBL1-17712 WB 1 0 0

Other Positive Controls
  • Recombinant human VEGF protein
  Peptides and Proteins (6)
Name Catalog# Reactivity Applications Images Publications Reviews
VEGF Protein NBP1-30236 Mu ELISA, PAGE 1 0 0

VEGF Protein NBP1-42890 IA 0 0 0

VEGF Protein NBP1-42891 IA 0 0 0

VEGF Protein NBP1-43188 IA 0 0 0

VEGF Protein NBP1-43189 IA 0 0 0

... and 1 more. See All »
  Primary Antibodies (19)
Name Catalog# Reactivity Applications Images Publications Reviews
VEGF Antibody (VG1) NB100-664 Hu, Mu, Rt, Ca WB, ICC/IF, IHC, IHC-Fr, IHC-P 1 18 0

VEGF Antibody (EP1176Y) NB110-57642 Hu, Mu(-), Rt(-) WB, FLOW, ICC/IF, IHC, IHC-P 4 1 0

VEGF Antibody (5C3.F8) NB600-548 Hu, Mu WB, IHC, IHC-P 1 3 0

VEGF Antibody NB100-698 Hu, Mu, Rt IHC, IHC-P 0 0 0

VEGF Antibody (6B7) NBP1-19181 Hu, Mu WB, IP 2 0 0

... and 14 more. See All »
  Secondary Antibodies (617)
Name Catalog# Reactivity Applications Images Publications Reviews
Rabbit anti-Mouse IgG Antibody [Alkaline Phosphatase] NB720-AP Mu WB, ELISA, ICC/IF, IHC, IHC-P 0 1 0

Rabbit anti-Mouse IgG Antibody [Biotin] NB720-B Mu WB, ELISA, IHC, IHC-P 0 0 0

Rabbit anti-Mouse IgG Antibody [HRP] NB720-H Mu WB, ELISA, ICC/IF, IHC, IHC-P 1 1 1

Goat anti-Mouse IgG Antibody [FITC] NB720-F Mu ICC/IF, IHC, IHC-P 0 3 0

Goat anti-Mouse IgG Antibody [HRP] NB7570 Mu WB, ELISA, ICC/IF, IHC, IHC-P 0 1 0

See All »
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VEGF in Context

 
Research Areas related to VEGF (5):
 
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