This VEGF antibody is useful for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Radioimmunoassay was reported in scientific literature. In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.
Reviews for VEGF Antibody (NB100-648) (5)
Read what people are saying who have used VEGF Antibody (NB100-648).
We have 5 reviews tested in 1 species: Other.
We have 5 reviews tested in 3: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry/Immunofluorescence.
Average Rating: 3.8 (Based on 5 reviews)
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This product has been successfully used in paraffin embedded tissue for fetal sheep brain (for the first time). The staining is faint, but consistent. A strong antigen retrieval (TBS pH 10) is required, but little background appears. Detection Method: DAB, Positive Control: Spiny Mouse Placenta, Negative Control: No Primary, Deparaffinization: Xylene, 100% Etoh, 70% Etoh, Antigen Retrieval: TBS (ph 10), 3 x 10 min in microwave at 100 degrees Celsius
Blocking Buffer: Normal Goat Serum Block Buffer, Blocking Time: 45 minutes, Blocking Temp: room temperature
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FAQs for VEGF (5)
Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?
We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.
Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?
The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
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