VEGF Antibody (VG76e) 0.1 ml

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Western Blot: VEGF Antibody (VG76e) [NB100-648] - Analysis of VEGF in human kidney protein (30ug).
Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.
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Ordering Information: NB100-648

  • Catalog Number
    NB100-648
  • Displayed Format
    Unconjugated
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VEGF Antibody (VG76e) Summary

Species Human, Porcine, Bovine, Sheep
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, RIA
Clonality
Monoclonal
Host
Mouse
Gene
VEGFA
Purity
Protein G purified
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VEGF Antibody (VG76e) Details

Clone
VG76e
Isotype
IgG1
Immunogen
Recombinant human VEGF189 expressed in E.coli [UniProt# P15692]
Localization
This antibody is highly specific, but only weakly stains transfected cells (5-10%).

Species Reactivity

Human. Mixed results in Cow and Pig (IHC only). Customers have reported success staining sheep tissue (see review).
Reviewed Species Read 5 rated 3.8
using NB100-648 in the following species:

Publications Read Publications using NB100-648 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 0.5-2ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
Application Notes
This VEGF antibody is useful for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Radioimmunoassay was reported in scientific literature. In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Molecular Weight
40 kDa
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-648AF405
Alexa Fluor (R) 488 Labeled NB100-648AF488
Alexa Fluor (R) 647 Labeled NB100-648AF647
Alexa Fluor (R) 700 Labeled NB100-648AF700
Allophycocyanin Labeled NB100-648APC
Biotin Labeled NB100-648B
DyLight 350 Labeled NB100-648UV
DyLight 405 Labeled NB100-648V
DyLight 405LS Labeled NB100-648V3
DyLight 488 Labeled NB100-648G
DyLight 550 Labeled NB100-648R
DyLight 650 Labeled NB100-648C
DyLight 680 Labeled NB100-648FR
DyLight 755 Labeled NB100-648IR
FITC Labeled NB100-648F
HRP Labeled NB100-648H
PE Labeled NB100-648PE
PerCP Labeled NB100-648PCP
Reviewed Applications
Read 5 Review rated 3.8
using
NB100-648 in the following applications:

Publications
Read Publications using
NB100-648 in the following applications:

  • 4 publications
  • 2 publications
  • 1 publication
  • 1 publication

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 3.8
Recent Reviews
IHC-P Tested In Other
Reviewed On: 07/29/2014
Rated: 4

VEGF NB100-648
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Publications

VEGF Antibody (NB100-648) has been mentioned in at least 11 publications.
Earn rewards if you have published using VEGF Antibody (NB100-648).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Unit Size
0.1 ml
Concentration
1 mg/ml
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol VEGFA
Entrez
Uniprot

Alternate Names for VEGF Antibody (VG76e)

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor
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Related Products by Gene

Background

VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.

Reviews for VEGF Antibody (NB100-648) (5) 3.85

Read what people are saying who have used VEGF Antibody (NB100-648).

We have 5 reviews tested in 1 species: Other.
We have 5 reviews tested in 4: Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry.
Average Rating: 3.8
(Based on 5 reviews)

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Sort 5 reviews for VEGF Antibody (NB100-648) by:
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Excellent
(1)
Very Good
(2)
Average
(2)
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Filter by Applications
WB
(1)
IHC-P
(2)
IF
(1)
ICC
(1)
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Filter by Species
Other
(5)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Average
WB Other Hima S 09/14/2014
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Summary

ApplicationWestern Blot
Sample TestedBovine Lysate
SpeciesOther
Other SpeciesBovine
Immunohistochemistry-Paraffin VEGF NB100-648
Click to Enlarge
Very Good
IHC-P Other Anonymous 07/29/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedsheep tonsil
SpeciesOther
Other Speciessheep
CommentsDid not work with low pH antigen retrieval, required overnight incubation of primary antibody.

Primary Anitbody

Dilution Ratio1:200

Details

Fixation DetailsFFPE pH 9 antigen retrieval overnight at 60C
Immunofluorescence VEGF NB100-648
Click to Enlarge
Average
IF Other Ewa Kaniewska 05/13/2014
Show

Summary

ApplicationImmunofluorescence
SpeciesOther
Other Speciesporicine
FileView PDF
  Excellent
ICC Other Ewa Kaniewska 05/13/2014
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Summary

ApplicationImmunocytochemistry
Sample Testedpig endothelial cells
SpeciesOther
Other Speciespig
Lot747/07
Immunohistochemistry-Paraffin VEGF NB100-648
Click to Enlarge
Very Good
IHC-P Other Ana Baburamani 05/04/2011
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFetal Sheep Brain Tissue
Sample PretreatmentHypoxia
SpeciesOther
Other SpeciesSheep, Spiny Mouse
Lot467/04
CommentsThis product has been successfully used in paraffin embedded tissue for fetal sheep brain (for the first time). The staining is faint, but consistent. A strong antigen retrieval (TBS pH 10) is required, but little background appears. Detection Method: DAB, Positive Control: Spiny Mouse Placenta, Negative Control: No Primary, Deparaffinization: Xylene, 100% Etoh, 70% Etoh, Antigen Retrieval: TBS (ph 10), 3 x 10 min in microwave at 100 degrees Celsius

Blocking

Blocking DetailsBlocking Buffer: Normal Goat Serum Block Buffer, Blocking Time: 45 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:200, Incubation Dilution Buffer: True Vision (Sapphire Bioscience), Incubation Time/Temp: overnight at 4C

Secondary Antibody

Secondary Manufacturer Cat#Vector

Details

Detection NotesPlease see Comments
Fixation DetailsSample Preparation: PFA fixed, parafin embedded, Deparaffinization: Xylene, 100% Etoh, 70% Etoh

Publications for VEGF Antibody (NB100-648) (11)

Read what people are writing who have used VEGF Antibody (NB100-648).

We have publications tested in 4 confirmed species: Human, Rat, Human , Porcine.

We have publications tested in 4 applications: WB, IHC, IHC-P, RIA.

Have you published using VEGF Antibody (NB100-648)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(4)
IHC
(2)
IHC-P
(1)
RIA
(1)
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Filter By Species
Human
(3)
Rat
(1)
Human
(1)
Porcine
(2)
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Publication Applications Species
Collingridge DR, Carroll VA, Glaser M, Aboagye EO, Osman S, Hutchinson OC, Barthel H, Luthra SK, Brady F, Bicknell R, Price P, Harris AL. The development of [124I]iodinated-VG76e: a novel tracer for imaging vascular endothelial growth factor in vivo using positron emission tomography. Cancer Res. 6220:5912-9. 2002 Oct 15. [PMID: 12384557] (RIA, Human) RIA Human
Brown NS, Streeter EH, Jones A, Harris AL, Bicknell R. Cooperative stimulation of vascular endothelial growth factor expression by hypoxia and reactive oxygen species: the effect of targeting vascular endothelial growth factor and oxidative stress in an orthotopic xenograft model of bladder carcinoma. Br J Cancer;929:1696-701. 2005 May 9. [PMID: 15841086]
Zhang L et al. Validation of anti-vascular endothelial growth factor anti-VEGF antibodies for immunohistochemical localization of VEGF in tissue sections: expression of VEGF in the human endometrium. J Pathol;1854:402-8. 1998 Aug. [PMID: 9828839] (IHC, Human ) IHC Human
Fraser HM, Dickson SE, Lunn SF, Wulff C, Morris KD, Carroll VA, Bicknell R. Suppression of luteal angiogenesis in the primate after neutralization of vascular endothelial growth factor. Endocrinology;1413:995-1000. 2000 Mar. [PMID: 10698175]
Mannheim D et al. Impaired myocardial perfusion reserve in experimental hypercholesterolemia is independent of myocardial neovascularization. Am J Physiol Heart Circ Physiol;2925:H2449-58. 2007 May. [PMID: 17208989] (WB, Porcine) WB Porcine
Yu R, Dhall D, Nissen NN et al. Pancreatic neuroendocrine tumors in glucagon receptor-deficient mice. PLoS ONE 20116(8). [PMID: 21853126]
Yoshizawa S, Brown A, Barchowsky A. Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation. Acta Biomater 2014 Feb 24 [PMID: 24512978] (WB, Human) WB Human
Yoshizawa S, Brown A, Barchowsky A, Sfeir C. Role of magnesium ions on osteogenic response in bone marrow stromal cells. Connect Tissue Res. 2014 Aug 01 [PMID: 25158202] (WB, Human) WB Human
Xu X, Mao W, Chai Y et al. Angiogenesis Inhibitor, Endostar, Prevents Vasa Vasorum Neovascularization in a Swine Atherosclerosis Model. J Atheroscler Thromb 2015 May 26 [PMID: 26016418] (WB) WB
Nishimiya K, Matsumoto Y, Shindo T et al. Association of Adventitial Vasa Vasorum and Inflammation With Coronary Hyperconstriction After Drug-Eluting Stent Implantation in Pigs In Vivo Circ. J. 2015 Jun 01 [PMID: 26027445] (IHC-P, Porcine) IHC-P Porcine
Iwai R, Nemoto Y, Nakayama Y. Preparation and characterization of directed, one-day-self-assembled millimeter-size spheroids of adipose-derived mesenchymal stem cells. J Biomed Mater Res A 2015 Sep 19 [PMID: 26386244] (IHC, Rat) IHC Rat

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for VEGF (3)

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
  2. I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?
    • We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.
  3. Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?
    • The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.

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Bioinformatics Tool for VEGF

Discover related pathways, diseases and genes to VEGF. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for VEGF

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The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

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Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
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HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

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