VEGF Antibody (VG1) 0.1 mg

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Western Blot: VEGF Antibody (VG1) [NB100-664] - HUVEC and 293T Cells tested at 1:1000. Image provided by verified customer review.
Immunohistochemistry-Paraffin: VEGF Antibody (VG1) [NB100-664] - Analysis of Vascular Endothelial GF formalin-fixed paraffin embedded human angiosarcoma.
Western Blot: VEGF Antibody (VG1) [NB100-664] - Western blot analysis of VEGF in human kidney protein using NB100-664.
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Ordering Information: NB100-664

  • Catalog Number
    NB100-664
  • Displayed Format
    Unconjugated
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VEGF Antibody (VG1) Summary

Species Human, Mouse, Rat, Canine
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Monoclonal
Host
Mouse
Gene
VEGFA
Purity
Protein G purified
Specificity
This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
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VEGF Antibody (VG1) Details

Clone
VG1
Isotype
IgG1 Kappa
Immunogen
Recombinant VEGF 189 protein.

Species Reactivity

Human, Mouse, Rat, and Dog.
Reviewed Species Read 1 rated 5
using NB100-664 in the following species:

Publications Read Publications using NB100-664 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:20-1:100
  • Immunohistochemistry-Frozen 1:20-1:100
  • Immunohistochemistry-Paraffin 1:20-1:100
Application Notes
This VEGF antibody is useful for Immunohistochemistry (frozen and paraffin-embedded sections), Western blot and Immunocytochemistry/Immunofluorescence. ELISA was reported in scientific literature. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining.
Positive Controls
Other Available
Formats
7C Labeled NB100-664V2
Alexa Fluor (R) 405 Labeled NB100-664AF405
Alexa Fluor (R) 488 Labeled NB100-664AF488
Alexa Fluor (R) 647 Labeled NB100-664AF647
Alexa Fluor (R) 700 Labeled NB100-664AF700
Allophycocyanin Labeled NB100-664APC
Biotin Labeled NB100-664B
DyLight 350 Labeled NB100-664UV
DyLight 405 Labeled NB100-664V
DyLight 405LS Labeled NB100-664V3
DyLight 488 Labeled NB100-664G
DyLight 550 Labeled NB100-664R
DyLight 650 Labeled NB100-664C
DyLight 680 Labeled NB100-664FR
DyLight 755 Labeled NB100-664IR
FITC Labeled NB100-664F
HRP Labeled NB100-664H
PE Labeled NB100-664PE
PerCP Labeled NB100-664PCP
Reviewed Applications
Read 1 rated 5
using
NB100-664 in the following applications:

Publications
Read Publications using
NB100-664 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 5
Recent Reviews
WB Tested In Human
Reviewed On: 02/28/2014
Rated: 5

VEGF NB100-664
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Publications

VEGF Antibody (NB100-664) has been mentioned in at least 22 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150mM NaCl pH 7.5
Unit Size
0.1 mg
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol VEGFA
Entrez
Uniprot

Alternate Names for VEGF Antibody (VG1)

  • MVCD1
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • VEGF-A
  • VEGFMGC70609
  • VPFvascular endothelial growth factor
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Related Products by Gene

Background

VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.

Reviews for VEGF Antibody (NB100-664) (1) 51

Read what people are saying who have used VEGF Antibody (NB100-664).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 5
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot VEGF NB100-664
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Excellent
WB Human Anonymous 02/28/2014
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Summary

ApplicationWestern Blot
Sample TestedHUVEC and 293T cells
SpeciesHuman

Blocking

Blocking Details100mL Tris buffered saline, 5g BSA, 0.1mL Tween 20. Incubate overnight at 4C

Primary Anitbody

Dilution RatioDilute 1:1000

Secondary Antibody

Secondary Manufacturer Cat#NBP1-75102
Secondary Concentration1:5000

Details

Detection NotesAmersham ECL, Hyperfilm ECL

Publications for VEGF Antibody (NB100-664) (22)

Read what people are writing who have used VEGF Antibody (NB100-664).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P.

Have you published using VEGF Antibody (NB100-664)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(4)
ELISA
(1)
ICC/IF
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(3)
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Filter By Species
Human
(4)
Mouse
(2)
Rat
(2)
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Publication Applications Species
Couvelard A, O'Toole D, Turley H, Leek R, Sauvanet A, Degott C, Ruszniewski P, Belghiti J, Harris AL, Gatter K, Pezzella F. Microvascular density and hypoxia-inducible factor pathway in pancreatic endocrine tumours: negative correlation of microvascular density and VEGF expression with tumour progression. Br J Cancer;92(1):94-101. 2005 Jan 17. [PMID: 15558070]
Hong DS, Kurzrock R, Oh Y et al. A Phase 1 Dose-Escalation, Pharmacokinetic, and Pharmacodynamic Evaluation of eIF-4E Antisense Oligonucleotide LY2275796 in Patients with Advanced Cancer. Clin Cancer Res. 2011 Aug 10. [PMID: 21831956]
Kakolyris S, Giatromanolaki A, Koukourakis M, Leigh IM, Georgoulias V, Kanavaros P, Sivridis E, Gatter KC, Harris AL. Assessment of vascular maturation in non-small cell lung cancer using a novel basement membrane component, LH39: correlation with p53 and angiogenic factor expression. Cancer Res;59(21):5602-7. 1999 Nov 1. [PMID: 10554041]
Adams J, Carder PJ, Downey S, Forbes MA, MacLennan K, Allgar V, Kaufman S, Hallam S, Bicknell R, Walker JJ, Cairnduff F, Selby PJ, Perren TJ, Lansdown M, Banks RE. Vascular endothelial growth factor (VEGF) in breast cancer: comparison of plasma, serum, and tissue VEGF and microvessel density and effects of tamoxifen. Cancer Res;60(11):2898-905. 2000 Jun 1. [PMID: 10850435]
Koukourakis MI, Giatromanolaki A, Thorpe PE, Brekken RA, Sivridis E, Kakolyris S, Georgoulias V, Gatter KC, Harris AL. Vascular endothelial growth factor/KDR activated microvessel density versus CD31 standard microvessel density in non-small cell lung cancer. Cancer Res;60(11):3088-95. 2000 Jun 1. [PMID: 10850461]
Salgado R, Benoy I, Weytjens R, Van Bockstaele D, Van Marck E, Huget P, Hoylaerts M, Vermeulen P, Dirix LY. Arterio-venous gradients of IL-6, plasma and serum VEGF and D-dimers in human cancer. Br J Cancer;87(12):1437-44. 2002 Dec 2. [PMID: 12454774]
Van der Auwera I, Van Laere SJ, Van den Eynden GG, Benoy I, van Dam P, Colpaert CG, Fox SB, Turley H, Harris AL, Van Marck EA, Vermeulen PB, Dirix LY. Increased angiogenesis and lymphangiogenesis in inflammatory versus noninflammatory breast cancer by real-time reverse transcriptase-PCR gene expression quantification. Clin Cancer Res;10(23):7965-71. 2004 Dec 1. [PMID: 15585631]
Giatromanolaki A, Koukourakis MI, Kakolyris S, Turley H, O'Byrne K, Scott PA, Pezzella F, Georgoulias V, Harris AL, Gatter KC. Vascular endothelial growth factor, wild-type p53, and angiogenesis in early operable non-small cell lung cancer. Clin Cancer Res;4(12):3017-24. 1998 Dec. [PMID: 9865915] (IHC, Human) IHC Human
Turley H, Scott PA, Watts VM, Bicknell R, Harris AL, Gatter KC. Expression of VEGF in routinely fixed material using a new monoclonal antibody VG1. J Pathol;186(3):313-8. 1998 Nov. [PMID: 10211122] (WB, IHC-P, ELISA, Human) ELISA, WB, IHC-P Human
O'Byrne KJ, Koukourakis MI, Giatromanolaki A, Cox G, Turley H, Steward WP, Gatter K, Harris AL. Vascular endothelial growth factor, platelet-derived endothelial cell growth factor and angiogenesis in non-small-cell lung cancer. Br J Cancer;82(8):1427-32. 2000 Apr. [PMID: 10780522] (IHC-P, WB, Human) WB, IHC-P Human
Giatromanolaki A, Koukourakis MI, Sivridis E, O'Byrne K, Cox G, Thorpe PE, Gatter KC, Harris AL. Coexpression of MUC1 glycoprotein with multiple angiogenic factors in non-small cell lung cancer suggests coactivation of angiogenic and migration pathways. Clin Cancer Res;6(5):1917-21. 2000 May. [PMID: 10815916]
Zhang HT, Scott PA, Morbidelli L, Peak S, Moore J, Turley H, Harris AL, Ziche M, Bicknell R. The 121 amino acid isoform of vascular endothelial growth factor is more strongly tumorigenic than other splice variants in vivo. Br J Cancer;83(1):63-8. 2000 Jul. [PMID: 10883669]
Giatromanolaki A, Sivridis E, Maltezos E, Papazoglou D, Simopoulos C, Gatter KC, Harris AL, Koukourakis MI. Hypoxia inducible factor 1alpha and 2alpha overexpression in inflammatory bowel disease. J Clin Pathol;56(3):209-13. 2003 Mar. [PMID: 12610101]
Giatromanolaki A, Koukourakis MI, Sivridis E, Turley H, Wykoff CC, Gatter KC, Harris AL. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer. J Pathol;200(2):222-8. 2003 Jun. [PMID: 12754744]
Kapsoritakis A, Sfiridaki A, Maltezos E, Simopoulos K, Giatromanolaki A, Sivridis E, Koukourakis MI. Vascular endothelial growth factor in inflammatory bowel disease. Int J Colorectal Dis. 18(5):418-22. PubMed PMID: 12761641. 2003 Sep. [PMID: 12761641]
Giatromanolaki A, Koukourakis MI, Simopoulos C, Polychronidis A, Sivridis E. Vascular endothelial growth factor (VEGF) expression in operable gallbladder carcinomas. Eur J Surg Oncol;29(10):879-83. 2003 Dec. [PMID: 14624781]
Ptaszynska MM, Pendrak ML, Bandle RW, Stracke ML, Roberts DD. Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells. Mol Cancer Res;6(3):352-63. 2008 Mar. [PMID: 18337445] (WB, Human) WB Human
Milanesi A, Lee JW, Li Z et al. Beta-Cell Regeneration Mediated by Human Bone Marrow Mesenchymal Stem Cells PLoS One 2012 [PMID: 22879915] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Kunze D, Wuttig D, Kausch I et al. Antisense-mediated inhibition of survivin, hTERT and VEGF in bladder cancer cells in vitro and in vivo. Int J Oncol 2008 May [PMID: 18425331] (IHC-P, Mouse) IHC-P Mouse
Fedorova LV, Sodhi K, Gatto-Weis C et al. Peroxisome Proliferator-Activated Receptor delta Agonist, HPP593, Prevents Renal Necrosis under Chronic Ischemia. PLoS One 2013 May 15 [PMID: 23691217] (WB, Rat) WB Rat
Johansen FF, Hasseldam H, Rasmussen RS et al. Drug-Induced Hypothermia as Beneficial Treatment before and after Cerebral Ischemia. Pathobiology. 2013 Aug 23 [PMID: 23989388]
Tam JCW, Ko CH, Lau KM et al. A Chinese 2-herb formula (NF3) promotes hindlimb ischemia-induced neovascularization and wound healing in diabetic rats. Journal of Diabetes and … 3/21/2014 [PMID: 24731763] (IHC-Fr, Rat) IHC-Fr Rat

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FAQs for VEGF (8)

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
  2. I would be very grateful if you could let me know a recommended dilution for the VEGF antibody antibody NB100-664
    • The recommended dilution for IHC is 1:20-1:50 in an ABC method. If instead you are testing on cell culture, I would recommend a starting optimization point of 1:10 -1:2000.
  3. I would like to know the aproximate dilution of the VEGF Antibody (NB100-664) to use it in a Western blot assay.
    • In regards to your inquiry about our VEGF antibody (NB100-664), I am showing from the lab data that they have tested dilutions in the range of 1:500-1:1000 for this antibody. The optimal dilution should depend on your samples and the level of expression of VEGF, but a good starting point would be 1:1000 and then optimize from there.
  4. What is the minimum amount of protein necessary for the detection of VEGF by Western blot using VEGF Antibody (NB100-664)?
    • I would suggest at minimum 10ug of protein per well but if you have enough sample you might want to run multiple wells with increasing amounts of protein to optimize the conditions in your hands. You could start with 5ug and do a couple of wells with 10ug, 20ug and 40ug. Also try using different dilutions of primary, start with 1:500-1:1000 and work your way up or down depending on what your blot looks like.
  5. I am interested in buying an antibody to VEGF to detect VEGF in human umbilical vein endothelial cells for western blotting and was wondering if you might have any recommendations? I was thinking about NB100-664. Do you have a recommended amount to use for western blotting? Could I ask you what concentration the antibody is at since it says 0.1mg so what total volume do you send? Also is this the most commonly purchase one for western blotting do you know?
    • We do not have a recommended dilution noted for this specific antibody, but our general recommendation for Western blot is 1:1000-1:2000.This is a good starting point, but we always recommend optimization by the end user, as the optimal dilution is to some extent dependent on the particular sample/assay. The concentration for this product is stated as 1 mg/ml, so 0.1 mg would be supplied in 0.1 ml.
  6. I am looking for a VEGF-A antibody for the IHC staining of mouse colon. Do you have any publications or application references for this product?
    • NB100-664 is one of our best sellers with great customer feedback and citations in at least 18 peer reviewed publications from highly esteemed researchers. As far as mouse tissues are concerned, I would recommend that you go through the following paper: Milanesi A, et al. Beta-Cell regeneration mediated by human bone marrow mesenchymal stem cells. PLoS One. 2012;7(8):e42177. doi: 10.1371/journal.pone.0042177. Epub 2012 Aug 7. PMID:22879915.
  7. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  8. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Applications IHC-P, IHC, WB

NBP1-19182
Western Blot: HGF Antibody (7-2) [NBP1-19182] Lane 1 hHGF 1 ug, Lane 2 hHGF 0.5 ug and Lane 3 hHGF 0.25 ug.Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - 2) at 10ug/ml.Immunohistochemistry-Paraffin: HGF Antibody (7-2) [NBP1-19182] - 2) at 10ug/ml.

Mouse Monoclonal
Species Human, Mouse
Applications IHC-P, IHC, WB

Bioinformatics Tool for VEGF

Discover related pathways, diseases and genes to VEGF. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Research Areas for VEGF

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Blogs on VEGF

Check out the latest blog posts on VEGF.
LYVE1 - It's Alive! It's a LYVE
LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is one of the most specific and widely used mammalian lymphatic endothelial markers.It is found in lymph nodes and at the luminal/abluminal surfaces of lymphatic vessels. It is a single-pass t...  Read full blog post.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

VEGF Receptors, Angiogenesis and Cancer
Vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2) are related family members of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. They are key regulators of physiological angiogenesis du...  Read full blog post.

Controlling the HIF-1 Switch
Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio...  Read full blog post.

The Role of the MMP2 Antibody in Colorectal Cancer Research
The enzymes of the Matrix Metalloproteinase (MMP) family assist in the degradation of the extracellular matrix, under both normal and pathological conditions. MMP antibodies have identified roles in a number of physiological processes, such as embryon...  Read full blog post.

HIF-1 Beta Antibody Studies and Developments in Hypoxia Research
We at Novus Biologicals have a large HIF-1 (Hypoxia Inducible Factor-1) antibody catalog, with twenty HIF-1 beta antibody products specifically targeted to the beta subunit of HIF-1, a heterodimer which is known to contribute to many human diseases ar...  Read full blog post.