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VEGF Antibody (VG1) 0.1mg

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Catalog Number  NB100-664
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Jump to Information on VEGF Antibody (NB100-664)

VEGF Antibody (VG1) Summary
 
Species Human, Mouse, Rat, Canine
Tested Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality Monoclonal
Host Mouse
Gene VEGFA
Purity Protein G purified
Specificity This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
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VEGF Antibody (VG1) Details
 
Clone VG1
Isotype IgG1 Kappa
Immunogen Recombinant VEGF 189 protein.
Species Reactivity
 
Human, Mouse, Rat, and Dog.
Publications Read 1 publication using NB100-664 in the following species:
 
Applications/Dilutions
 
Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:20-1:100
  • Immunohistochemistry-Frozen 1:20-1:100
  • Immunohistochemistry-Paraffin 1:20-1:100
Application Notes

This VEGF antibody is useful for Immunohistochemistry (frozen and paraffin-embedded sections), Western blot and Immunocytochemistry/Immunofluorescence. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining.
Positive Controls

1 Positive Control
Directly Conjugated
Formats
DyLight 550 Labeled NB100-664R
HRP Labeled NB100-664H
DyLight 488 Labeled NB100-664G
DyLight 650 Labeled NB100-664C
Biotin Labeled NB100-664B
Learn about Custom Antibody Labeling
Publications
Read 1 publication using
NB100-664 in the following applications:
Product PDFs
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Reviews
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Publications
VEGF Antibody (NB100-664) has been mentioned in at least 18 publications.
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Protocols and FAQs
Controls & Support Products
View all related VEGF Controls and Support Products.
Research Tools
View all VEGF Research Tools.
Packaging, Storage & Formulations
 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150mM NaCl pH 7.5
Unit Size 0.1mg (also available in 0.025mg (sample size))
Concentration 1 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt.
Bioinformatics
 
Gene Symbol VEGFA
Entrez
Uniprot
Alternate Names for VEGF
 
  • MVCD1
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • VEGF-A
  • VEGFMGC70609
  • VPFvascular endothelial growth factor
Related Products by Gene
 
FGF2, FLT1, KDR, HIF1A, IL6, VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2, TNF, VEGF-A, VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-1, ANGPT1, PECAM1, BASIC FIBROBLAST GROWTH FACTOR, EGFR, AKT1, HBA1, CD34, PGF, MAPK3, MAPK1, HGF
Background
 
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.