VEGF Antibody (VG1) 0.1 mg

VEGF NB100-664

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Ordering Information: NB100-664

Catalog NumberNB100-664
  • FormUnconjugated
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VEGF Antibody (VG1) Summary

 
Species Human, Mouse, Rat, Canine
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality Monoclonal
Host Mouse
Gene VEGFA
Purity Protein G purified
Specificity This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
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VEGF Antibody (VG1) Details

 
Clone VG1
Isotype IgG1 Kappa
Immunogen Recombinant VEGF 189 protein.

Species Reactivity

 
Human, Mouse, Rat, and Dog.
Reviewed Species Read 1 rated 5  using NB100-664 in the following species:
 
Publications Read 7 using NB100-664 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:20-1:100
  • Immunohistochemistry-Frozen 1:20-1:100
  • Immunohistochemistry-Paraffin 1:20-1:100
Application Notes

This VEGF antibody is useful for Immunohistochemistry (frozen and paraffin-embedded sections), Western blot and Immunocytochemistry/Immunofluorescence. ELISA was reported in scientific literature. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining.

Positive Controls

1 Positive Control

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-664AF405
Alexa Fluor (R) 488 Labeled NB100-664AF488
Alexa Fluor (R) 647 Labeled NB100-664AF647
Alexa Fluor (R) 700 Labeled NB100-664AF700
Biotin Labeled NB100-664B
DyLight 405 Labeled NB100-664V
DyLight 488 Labeled NB100-664G
DyLight 550 Labeled NB100-664R
DyLight 650 Labeled NB100-664C
FITC Labeled NB100-664F
HRP Labeled NB100-664H
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 5  using
NB100-664 in the following application:
Publications
Read 7 publications using
NB100-664 in the following applications:
  • WB5publications

Product PDFs

Reviews

Average Rating: 5 
Recent Reviews

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Publications

VEGF Antibody (NB100-664) has been mentioned in at least 21 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all VEGF Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150mM NaCl pH 7.5
Unit Size 0.1 mg (also available in 0.025 mg (sample size))
Concentration 1 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol VEGFA
Entrez
Uniprot

Alternate Names for VEGF

 
  • Vascular permeability factor
  • VEGF-A
  • VEGFMGC70609
  • VPFvascular endothelial growth factor
  • MVCD1
  • vascular endothelial growth factor A

Related Products by Gene

Background

 
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.