VDR/NR1I1/Vitamin D Receptor Antibody 0.05 mg

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Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody [NBP1-61794] - Analysis of extracts from MCF-7 cells, using Vitamin D Receptor  Antibody. The lane on the right is treated with the synthesized peptide.
Immunocytochemistry/Immunofluorescence: VDR/NR1I1/Vitamin D Receptor Antibody [NBP1-61794] - Analysis of HeLa cells, using Vitamin D Receptor  Antibody. The picture on the right is treated with the synthesized peptide.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody [NBP1-61794] - Analysis of paraffin-embedded human thyroid gland tissue, using Vitamin D Receptor  Antibody. The picture on the right is treated with the synthesized peptide.
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Ordering Information: NBP1-61794

  • Catalog Number
    NBP1-61794
  • Displayed Format
    Unconjugated
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VDR/NR1I1/Vitamin D Receptor Antibody Summary

Species Human
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
VDR
Purity
Immunogen affinity purified
Specificity
Vitamin D Receptor (Ab-208) Antibody detects endogenous levels of total Vitamin D Receptor protein.
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VDR/NR1I1/Vitamin D Receptor Antibody Details

Immunogen
The antiserum was produced against synthesized peptide derived from human Vitamin D Receptor. The immunogen region is: Internal.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Molecular Weight
48 kDa

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Product PDFs

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Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Unit Size
0.05 mg
Concentration
1 mg/ml
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol VDR
Entrez
Uniprot

Alternate Names for VDR/NR1I1/Vitamin D Receptor Antibody

  • NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor
  • vitamin D1,25- dihydroxyvitamin D3 receptor
  • vitamin D3 receptor

Related Products by Gene

Background

The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally-specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9- cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.

Reviews for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794) (0)

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Publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794) (0)

There are no publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794).
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for VDR/NR1I1/Vitamin D Receptor (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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