VDR/NR1I1/Vitamin D Receptor Antibody 0.05 mg

Click image to view larger
Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody [NBP1-61794] - Analysis of extracts from MCF-7 cells, using Vitamin D Receptor  Antibody. The lane on the right is treated with the synthesized peptide.
Immunocytochemistry/Immunofluorescence: VDR/NR1I1/Vitamin D Receptor Antibody [NBP1-61794] - Analysis of HeLa cells, using Vitamin D Receptor  Antibody. The picture on the right is treated with the synthesized peptide.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody [NBP1-61794] - Analysis of paraffin-embedded human thyroid gland tissue, using Vitamin D Receptor  Antibody. The picture on the right is treated with the synthesized peptide.
Download Datasheet
See All Related

Ordering Information: NBP1-61794

  • Catalog Number
    NBP1-61794
  • Displayed Format
    Unconjugated
  • Size(s)
  • Availability
  • Price
    Shipping
  

VDR/NR1I1/Vitamin D Receptor Antibody Summary

Species Human
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
VDR
Purity
Immunogen affinity purified
Specificity
Vitamin D Receptor (Ab-208) Antibody detects endogenous levels of total Vitamin D Receptor protein.
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

VDR/NR1I1/Vitamin D Receptor Antibody Details

Immunogen
The antiserum was produced against synthesized peptide derived from human Vitamin D Receptor. The immunogen region is: Internal.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Molecular Weight
48 kDa

Contact Information

Product PDFs

Reviews

Earn rewards points by submitting your review for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794). Be the first to review this product and earn double rewards points!

Publications

Earn rewards if you have published using VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Unit Size
0.05 mg
Concentration
1 mg/ml
Preservative
0.02% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol VDR
Entrez
Uniprot

Alternate Names for VDR/NR1I1/Vitamin D Receptor Antibody

  • NR1I1
  • NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor
  • vitamin D1,25- dihydroxyvitamin D3 receptor
  • vitamin D3 receptor
Show More

Related Products by Gene

Background

The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally-specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9- cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.

Reviews for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794) (0)

There are no reviews for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794) (0)

There are no publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794).
By submitting a publication earn 200 points for your submission points towards our Rewards Program.
Have you published using VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794)? Earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for VDR/NR1I1/Vitamin D Receptor (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NBP2-35212
SDS-Page: Human PTH Protein [NBP2-35212]


Species Human
Applications Func, PAGE

H00001591-M02
Sandwich ELISA: CYP24A1 Antibody (1E1) [H00001591-M02] - Detection limit for recombinant GST tagged CYP24A1 is approximately 1ng/ml as a capture antibody.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, S-ELISA

     1 Review

1 Publication
MAB1419
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)Immunocytochemistry Osteocalcin Antibody (190125)

Mouse Monoclonal
Species Human, Rat
Applications IHC, ICC, ICFlow

     2 Reviews

6 Publications
NB120-7164

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications ELISA, ICC/IF, MiAr

2 Publications
NB300-164
Western Blot: Bestrophin 1 Antibody (E6-6) [NB300-164] - Detection of Bestrophin (68 kDa) from human RPE cell lysate.

Mouse Monoclonal
Species Human, Rat (Negative)
Applications WB, ICC/IF, IHC-Fr...MORE

19 Publications
NBP1-86713
Immunohistochemistry: PTRH1 Antibody [NBP1-86713] - Staining of human kidney shows strong cytoplasmic positivity in tubular cells.

Rabbit Polyclonal
Species Human
Applications IHC




NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human urinary transitional cell carcinoma stained with p21 antibody at 1:50 using peroxidase-conjugate and DAB chromogen.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ELISA, Flow...MORE

8 Publications
NBP2-29942
Western Blot: CYP27B1 Antibody [NBP2-29942] - Western blot analysis of (C-term) (NBP2-29942) in mouse kidney tissue lysates (35ug/lane). CYP27B1 (arrow) was detected using the purified Pab.Immunohistochemistry-Paraffin: CYP27B1 Antibody [NBP2-29942] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of  (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Flow Cytometry: CYP27B1 Antibody [NBP2-29942] - Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Rabbit Polyclonal
Species Human
Applications WB, Flow, IHC-P

NB300-560
Western Blot: Estrogen Receptor alpha Antibody (33) [NB300-560] - Recombinant ER alpha.Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).Immunohistochemistry: Estrogen Receptor alpha Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, B/N, Flow...MORE

1 Publication
H00008856-T01
NR1I2 transfected lysate.Lane 1: NR1I2 transfected lysate (47.85 KDa) Lane 2: Non-transfected lysate.


Species Human
Applications WB

NB120-19347
Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - In HEK7-2 cell extract using NB120-19347. Figure 2 shows Immunofluorescence of Bovine CE (Corneal Epithelium) Limbus tissue extracts.Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Bovine corneal epithelium (CE) limbus tissue.Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] -  Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ELISA, Flow...MORE

10 Publications



NB200-322
Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC-Fr

     1 Review

1 Publication
NB100-56603
Western Blot: Androgen Receptor [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - LNCaP cells (passage number 38) were serum-starved for 2 days.  After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h. (See Lin, H-K., Y-C Hu, L. Yang, S. Altuwaijri. 2003 Fig. 2)

Mouse Monoclonal
Species Human, Primate
Applications WB, IHC, IHC-P

12 Publications



PP-N4111-00

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

4 Publications
AF5520
Western Blot TRAP220/MED1 Antibody

Goat Polyclonal
Species Human, Mouse
Applications WB

     1 Review

NBP1-86664
Western Blot: Calbindin D-28K Antibody [NBP1-86664] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissueImmunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Immunofluorescent staining of human cell line U-251MG shows positivity in vesicles.Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

Bioinformatics Tool for VDR/NR1I1/Vitamin D Receptor

Discover related pathways, diseases and genes to VDR/NR1I1/Vitamin D Receptor. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Research Areas for VDR/NR1I1/Vitamin D Receptor

Find related products by research area.

Blogs on VDR/NR1I1/Vitamin D Receptor

There are no specific blogs for VDR/NR1I1/Vitamin D Receptor, but you can read our latest blog posts.