VDR/NR1I1/Vitamin D Receptor Antibody 0.05 mg

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Ordering Information: NBP1-61794

  • Catalog Number
    NBP1-61794
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 mg
  • Availability


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    VDR/NR1I1/Vitamin D Receptor Antibody Summary

    Species Human
    Tested Applications WB, ELISA, ICC/IF, IHC, IHC-P
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    VDR
    Purity
    Immunogen affinity purified
    Specificity
    Vitamin D Receptor (Ab-208) Antibody detects endogenous levels of total Vitamin D Receptor protein.
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    VDR/NR1I1/Vitamin D Receptor Antibody Details

    Immunogen
    The antiserum was produced against synthesized peptide derived from human Vitamin D Receptor. The immunogen region is: Internal.

    Applications/Dilutions

    Dilutions
    • Western Blot 1:500-1:1000
    • ELISA 1:10000
    • Immunocytochemistry/Immunofluorescence 1:100-1:500
    • Immunohistochemistry 1:50-1:100
    • Immunohistochemistry-Paraffin 1:50-1:100
    Application Notes
    The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    Molecular Weight
    48 kDa

    Contact Information

    Product PDFs

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    Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
    Unit Size
    0.05 mg
    Concentration
    1 mg/ml
    Preservative
    0.02% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol VDR
    Entrez
    Uniprot

    Alternate Names for VDR/NR1I1/Vitamin D Receptor Antibody

    • NR1I1
    • NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor
    • vitamin D1,25- dihydroxyvitamin D3 receptor
    • vitamin D3 receptor
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    Related Products by Gene

    Background

    The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally-specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9- cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.

    Reviews for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794) (0)

    There are no reviews for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794).
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    Publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794) (0)

    There are no publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794).
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    Have you published using VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-61794)? Earn rewards points which can be used for merchandise & discounts.
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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol
    ICC/IF Video Protocol

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    FAQs for VDR/NR1I1/Vitamin D Receptor (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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