USP22 Antibody 0.025 ml

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Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.
Western Blot: USP22 Antibody [NBP1-49644] - Western  blot analysis of Usp22 in HeLa whole cell extracts.
Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NBP1-49644

  • Catalog Number
    NBP1-49644
  • Displayed Format
    Unconjugated
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USP22 Antibody Summary

Species Human, Mouse
Tested Applications WB, Simple Western, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
USP22
Purity
Immunogen affinity purified
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USP22 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human USP22 protein (within residues 100-205). [Swiss-Prot Q9H3R0]
Localization
Nucleus.

Species Reactivity

Human and mouse.
Reviewed Species Read 1 rated 5
using NBP1-49644 in the following species:

Publications Read Publications using NBP1-49644 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunohistochemistry-Paraffin
Application Notes
This Usp22 antibody is useful for Western blot, where a band is seen ~59 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24197134)In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-49644AF405
Alexa Fluor (R) 488 Labeled NBP1-49644AF488
Alexa Fluor (R) 647 Labeled NBP1-49644AF647
Alexa Fluor (R) 700 Labeled NBP1-49644AF700
Biotin Labeled NBP1-49644B
DyLight 405 Labeled NBP1-49644V
DyLight 488 Labeled NBP1-49644G
DyLight 550 Labeled NBP1-49644R
DyLight 650 Labeled NBP1-49644C
FITC Labeled NBP1-49644F
HRP Labeled NBP1-49644H
Reviewed Applications
Read 1 rated 5
using
NBP1-49644 in the following applications:

Publications
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NBP1-49644 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 5
Recent Reviews
WB Tested In Human
Reviewed On: 11/25/2014
Rated: 5

USP22 NBP1-49644
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Publications

USP22 Antibody (NBP1-49644) has been mentioned in at least 1 publication.
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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol USP22
Entrez
Uniprot

Alternate Names for USP22 Antibody

  • Deubiquitinating enzyme 22
  • EC 3.1.2.15
  • EC 3.4.19.12
  • KIAA1063ubiquitin carboxyl-terminal hydrolase 22
  • KIAA1064
  • ubiquitin specific peptidase 22
  • ubiquitin specific peptidase 3-like
  • ubiquitin specific protease 22
  • ubiquitin thioesterase 22
  • Ubiquitin thiolesterase 22
  • ubiquitin-specific processing protease 22
  • Ubiquitin-specific-processing protease 22
  • USP3L
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Related Products by Gene

Background

USP22 is the component of the histone acetylation complex, SAGA, that acts as a deubiquinating enzyme. It is a cofactor for gene promoters like MYC. Depleting USP22 prevents MYC from controlling the transcription and expression of thousands of other genes. Therefore, it can be postulated that USP22 is the critical mediator of MYC induced oncogenesis.

Reviews for USP22 Antibody (NBP1-49644) (1) 51

Read what people are saying who have used USP22 Antibody (NBP1-49644).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 5
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot USP22 NBP1-49644
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Excellent
WB Human 11/25/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
LotA-1
Special ApplicationsHeLa Whole cell lysate, Antibody dilution used is 1:500 overnight 4 degree
FileView PDF

Publications for USP22 Antibody (NBP1-49644) (1)

Read what people are writing who have used USP22 Antibody (NBP1-49644).

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: WB, IHC-P.

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Product General Protocols

View specific protocols for USP22 Antibody (NBP1-49644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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FAQs for USP22 (31)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
  3. What epitope does this monoclonal antibody recognize?
    • R&D Systems does not epitope map its antibodies. The antibody is generated using the entire immunogen listed on the datasheet. If a peptide immunogen is used, this will be indicated on the datasheet.
  4. What epitope does this polyclonal antibody recognize?
    • Polyclonal antibodies generally recognize multiple epitopes because they are generated using the entire immunogen stated on the product datasheet.
  5. What are R&D Systems matched antibody pairs?
    • R&D Systems matched antibody pairs assist in the development of an ELISA. R&D Systems has demonstrated that the paired antibodies can be used in a sandwich immunoassay to recognize the recombinant protein. We recommend that the investigator have expertise in immunoassay development before attempting to use these products. Each investigator must empirically determine the optimal concentrations for the capture and detection antibodies. The amount of antibodies supplied are not normalized to develop the same number of plates. R&D Systems recombinant cytokines are not calibrated by ELISA and therefore must be mass calibrated before use. The DuoSet ELISA Development Kit product line includes the antibodies, standard, enzyme, and protocol necessary for running an immunoassay, and can often be a better value than purchasing antibody pairs and protein standard.
  6. Can I use your antibodies for a matched pair ELISA with a standard from another company?
    • Yes, but there are potential issues to consider. There can be differences in immunological recognition or the binding between antibody and recombinant protein. This can happen if there is a difference in the folding or sequence of the recombinant protein used as the standard versus the recombinant protein used as the immunogen for the antibody. Protein folding or sequence differences in the antibody-binding region can lead to poor (or no) recognition of the standard.
  7. Can I use your antibodies for a matched pair ELISA with a standard from another company?
    • Yes, but there are potential issues to consider. There can be differences in immunological recognition or the binding between antibody and recombinant protein. This can happen if there is a difference in the folding or sequence of the recombinant protein used as the standard versus the recombinant protein used as the immunogen for the antibody. Protein folding or sequence differences in the antibody-binding region can lead to poor (or no) recognition of the standard.
  8. What grade BSA is recommend for use in ELISA and ELISpot Development Systems?
    • The grade of the BSA used has been found to be a critical component for running a successful ELISA. BSA with a minimum purity of 98% is recommended. R&D Systems recommends using the same BSAs used in development of the DuoSet; Catalog # DY995 Reagent Diluent Concentrate 2, Catalog # DY997 Reagent Diluent Concentrate 1. Millipore Probumin® Diagnostic Grade BSA (Millipore, Catalog # 82-045) is another example.
  9. What grade BSA is recommend for use in ELISA and ELISpot Development Systems?
    • The grade of the BSA used has been found to be a critical component for running a successful ELISA. BSA with a minimum purity of 98% is recommended. R&D Systems recommends using the same BSAs used in development of the DuoSet; Catalog # DY995 Reagent Diluent Concentrate 2, Catalog # DY997 Reagent Diluent Concentrate 1. Millipore Probumin® Diagnostic Grade BSA (Millipore, Catalog # 82-045) is another example.
  10. What is a direct ELISA?
    • In a direct ELISA, a plate is coated with the analyte of interest and a labeled detection antibody is used to verify the presence of the analyte.The direct ELISA may use a colorimetric, chemiluminescent, or fluorescent reporter.
  11. What is a direct ELISA?
    • In a direct ELISA, a plate is coated with the analyte of interest and a labeled detection antibody is used to verify the presence of the analyte.The direct ELISA may use a colorimetric, chemiluminescent, or fluorescent reporter.
  12. What is a sandwich ELISA?
    • A sandwich ELISA uses an immobilized capture antibody specific for the analyte of interest in a sample. After the analyte is bound to the immobilized antibody, a labeled secondary antibody specific for the analyte is used for detection. The analyte is "sandwiched" between the two antibodies. The sandwich ELISA is extremely sensitive, and the values obtained are quantitative when compared with a standard curve.
  13. What is a sandwich ELISA?
    • A sandwich ELISA uses an immobilized capture antibody specific for the analyte of interest in a sample. After the analyte is bound to the immobilized antibody, a labeled secondary antibody specific for the analyte is used for detection. The analyte is "sandwiched" between the two antibodies. The sandwich ELISA is extremely sensitive, and the values obtained are quantitative when compared with a standard curve.
  14. What is the difference between AB###, AF###, BAF###, MAB###, and BAM### antibody catalog number designations?
    • AB### designated antibodies are polyclonal antibodies that are purified using Protein A, Protein G or Ion Exchange chromatography. Consequently, the resulting purified antibody is a total IgG fraction and may contain IgG not specific for the analyte of interest. AF### designated antibodies, are antigen affinity-purified and then further purified by ion exchange or size exclusion. Therefore, AF### antibodies are IgG specific only to the antigen. Antibodies that have the designation MAB### are monoclonal antibodies. BAF### and BAM### designated antibodies are the biotinylated versions of the AF### and MAB### designated antibodies, respectively.
  15. What is the difference between AB###, AF###, BAF###, MAB###, and BAM### antibody catalog number designations?
    • AB### designated antibodies are polyclonal antibodies that are purified using Protein A, Protein G or Ion Exchange chromatography. Consequently, the resulting purified antibody is a total IgG fraction and may contain IgG not specific for the analyte of interest. AF### designated antibodies, are antigen affinity-purified and then further purified by ion exchange or size exclusion. Therefore, AF### antibodies are IgG specific only to the antigen. Antibodies that have the designation MAB### are monoclonal antibodies. BAF### and BAM### designated antibodies are the biotinylated versions of the AF### and MAB### designated antibodies, respectively.
  16. What is the rationale for using trehalose to stabilize proteins?
    • Trehalose is an effective sugar for stabilizing proteins against damage caused by freezing. It can also make the protein more resistant to moisture gain when lyophilized, resulting in a product that is less likely to precipitate when reconstituted. In addition, it has been used in approved parenteral therapeutics
  17. What is the rationale for using trehalose to stabilize proteins?
    • Trehalose is an effective sugar for stabilizing proteins against damage caused by freezing. It can also make the protein more resistant to moisture gain when lyophilized, resulting in a product that is less likely to precipitate when reconstituted. In addition, it has been used in approved parenteral therapeutics
  18. Will trehalose affect my conjugation reaction?
    • It is possible that the presence of trehalose will interfere in the successful conjugation of a protein. This will depend on the method used, and the customer should investigate this potential prior to purchasing the product.
  19. Will trehalose affect my conjugation reaction?
    • It is possible that the presence of trehalose will interfere in the successful conjugation of a protein. This will depend on the method used, and the customer should investigate this potential prior to purchasing the product.
  20. Will trehalose affect the performance of the protein or antibody in my specific application?
    • We have seen no adverse effect in our bioassays or other approved applications. However, customers are advised to run a control in their assay to determine if the concentration of trelahose in the protein or antibody formulation has any adverse effects.
  21. Will trehalose affect the performance of the protein or antibody in my specific application?
    • We have seen no adverse effect in our bioassays or other approved applications. However, customers are advised to run a control in their assay to determine if the concentration of trelahose in the protein or antibody formulation has any adverse effects.
  22. Will trehalose included in the formulation affect the animal if it is injected?
    • Trehalose is unlikely to have an effect in vivo. It has been approved as an excipient for use in human injectable drugs.The trehalose used by R&D Systems is derived from Saccharomyces cerevisiae and is determined to be at minimum 98.5% pure by HPAE.
  23. Will trehalose included in the formulation affect the animal if it is injected?
    • Trehalose is unlikely to have an effect in vivo. It has been approved as an excipient for use in human injectable drugs.The trehalose used by R&D Systems is derived from Saccharomyces cerevisiae and is determined to be at minimum 98.5% pure by HPAE.
  24. What are R&D Systems matched antibody pairs?
    • R&D Systems matched antibody pairs assist in the development of an ELISA. R&D Systems has demonstrated that the paired antibodies can be used in a sandwich immunoassay to recognize the recombinant protein. We recommend that the investigator have expertise in immunoassay development before attempting to use these products. Each investigator must empirically determine the optimal concentrations for the capture and detection antibodies. The amount of antibodies supplied are not normalized to develop the same number of plates. R&D Systems recombinant cytokines are not calibrated by ELISA and therefore must be mass calibrated before use. The DuoSet ELISA Development Kit product line includes the antibodies, standard, enzyme, and protocol necessary for running an immunoassay, and can often be a better value than purchasing antibody pairs and protein standard.
  25. What is the recommended protocol for reconstituting and aliquotting lyophilized proteins and antibodies?<br>
    • Following these guidelines for reconstituting lyophilized material will help ensure complete recovery of the protein or antibody.

      1) Tap or briefly centrifuge the vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.

      2) Use the buffer and stock concentration recommended in the product datasheet. If you want a stock concentration that is higher than the one recommended, contact Technical Service for specific recommendations.

      3) For optimal recovery, reconstitute using room temperature buffer.

      4) After adding the buffer, re-cap the vial and invert gently by hand or place on a slow rocking platform. This will allow the reconstitution buffer to coat all the surfaces inside the vial. Do not mix by vortexing or by pipetting the material up and down.

      5) Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.

      6) Store the reconstituted protein in polypropylene or siliconized tubes. If aliquotting, it is recommended that aliquots be no smaller than 10 μL. In addition, avoid repeated freeze/thaw cycles.

  26. What is the recommended protocol for reconstituting and aliquotting lyophilized proteins and antibodies?<br>
    • Following these guidelines for reconstituting lyophilized material will help ensure complete recovery of the protein or antibody.

      1) Tap or briefly centrifuge the vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.

      2) Use the buffer and stock concentration recommended in the product datasheet. If you want a stock concentration that is higher than the one recommended, contact Technical Service for specific recommendations.

      3) For optimal recovery, reconstitute using room temperature buffer.

      4) After adding the buffer, re-cap the vial and invert gently by hand or place on a slow rocking platform. This will allow the reconstitution buffer to coat all the surfaces inside the vial. Do not mix by vortexing or by pipetting the material up and down.

      5) Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.

      6) Store the reconstituted protein in polypropylene or siliconized tubes. If aliquotting, it is recommended that aliquots be no smaller than 10 μL. In addition, avoid repeated freeze/thaw cycles.

  27. Can this antibody be used on frozen or paraffin-embedded&nbsp; sections?
    • R&D Systems will provide support for an antibody that is validated for the IHC/ICC application in both frozen and paraffin-embedded sections unless otherwise specified on the datasheet. Should the product fail to work after contacting us for assistance, R&D Systems will offer a product credit toward a future purchase.

      If the datasheet does not have an IHC/ICC claim, we do not have any in-house or collaborative data available to support this application and we cannot guarantee results. It is up to the user to decide if they wish to use the antibody in their application.

      Customers may use the Citations tab on the product-specific web page or contact Technical Service to check for references using a product in an application or with a cell type R&D Systems has not tested in-house.
  28. Can this antibody be used on frozen or paraffin-embedded&nbsp; sections?
    • R&D Systems will provide support for an antibody that is validated for the IHC/ICC application in both frozen and paraffin-embedded sections unless otherwise specified on the datasheet. Should the product fail to work after contacting us for assistance, R&D Systems will offer a product credit toward a future purchase.

      If the datasheet does not have an IHC/ICC claim, we do not have any in-house or collaborative data available to support this application and we cannot guarantee results. It is up to the user to decide if they wish to use the antibody in their application.

      Customers may use the Citations tab on the product-specific web page or contact Technical Service to check for references using a product in an application or with a cell type R&D Systems has not tested in-house.
  29. Can this antibody be used on frozen or paraffin-embedded&nbsp; sections?
    • R&D Systems will provide support for an antibody that is validated for the IHC/ICC application in both frozen and paraffin-embedded sections unless otherwise specified on the datasheet. Should the product fail to work after contacting us for assistance, R&D Systems will offer a product credit toward a future purchase.

      If the datasheet does not have an IHC/ICC claim, we do not have any in-house or collaborative data available to support this application and we cannot guarantee results. It is up to the user to decide if they wish to use the antibody in their application.

      Customers may use the Citations tab on the product-specific web page or contact Technical Service to check for references using a product in an application or with a cell type R&D Systems has not tested in-house.
  30. Has a product ever been used for an application that is not listed on the datasheet?
    • If a specific application is not listed on the datasheet, it may mean that this product has not been tested in this application, or it may mean that in-house testing in this application did not meet R&D Systems’specifications. Please check our Citations tab to see if other researchers have published using your application, sample type and/or species. If you would like more information on whether or not an application has been tested, please contact Technical Service at (800) 343-7475.

  31. Has a product ever been used for an application that is not listed on the datasheet?
    • If a specific application is not listed on the datasheet, it may mean that this product has not been tested in this application, or it may mean that in-house testing in this application did not meet R&D Systems’specifications. Please check our Citations tab to see if other researchers have published using your application, sample type and/or species. If you would like more information on whether or not an application has been tested, please contact Technical Service at (800) 343-7475.