USP22 Antibody 0.025 ml

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Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.
Western Blot: USP22 Antibody [NBP1-49644] - Western  blot analysis of Usp22 in HeLa whole cell extracts.
Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NBP1-49644

  • Catalog Number
    NBP1-49644
  • Displayed Format
    Unconjugated
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USP22 Antibody Summary

Species Human, Mouse
Tested Applications WB, Simple Western, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
USP22
Purity
Immunogen affinity purified
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USP22 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human USP22 protein (within residues 100-205). [Swiss-Prot Q9H3R0]
Localization
Nucleus.

Species Reactivity

Human and mouse.
Reviewed Species Read 1 rated 5
using NBP1-49644 in the following species:

Publications Read Publications using NBP1-49644 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunohistochemistry-Paraffin
Application Notes
This Usp22 antibody is useful for Western blot, where a band is seen ~59 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24197134)In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-49644AF405
Alexa Fluor (R) 488 Labeled NBP1-49644AF488
Alexa Fluor (R) 647 Labeled NBP1-49644AF647
Alexa Fluor (R) 700 Labeled NBP1-49644AF700
Biotin Labeled NBP1-49644B
DyLight 405 Labeled NBP1-49644V
DyLight 488 Labeled NBP1-49644G
DyLight 550 Labeled NBP1-49644R
DyLight 650 Labeled NBP1-49644C
FITC Labeled NBP1-49644F
HRP Labeled NBP1-49644H
Reviewed Applications
Read 1 rated 5
using
NBP1-49644 in the following applications:

Publications
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NBP1-49644 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 5
Recent Reviews
WB Tested In Human
Reviewed On: 11/25/2014
Rated: 5

USP22 NBP1-49644
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Publications

USP22 Antibody (NBP1-49644) has been mentioned in at least 1 publication.
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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol USP22
Entrez
Uniprot

Alternate Names for USP22 Antibody

  • Deubiquitinating enzyme 22
  • EC 3.1.2.15
  • EC 3.4.19.12
  • KIAA1063ubiquitin carboxyl-terminal hydrolase 22
  • KIAA1064
  • ubiquitin specific peptidase 22
  • ubiquitin specific peptidase 3-like
  • ubiquitin specific protease 22
  • ubiquitin thioesterase 22
  • Ubiquitin thiolesterase 22
  • ubiquitin-specific processing protease 22
  • Ubiquitin-specific-processing protease 22
  • USP3L
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Background

USP22 is the component of the histone acetylation complex, SAGA, that acts as a deubiquinating enzyme. It is a cofactor for gene promoters like MYC. Depleting USP22 prevents MYC from controlling the transcription and expression of thousands of other genes. Therefore, it can be postulated that USP22 is the critical mediator of MYC induced oncogenesis.

Reviews for USP22 Antibody (NBP1-49644) (1) 51

Read what people are saying who have used USP22 Antibody (NBP1-49644).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 5
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot USP22 NBP1-49644
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Excellent
WB Human Vijayalingam Selvamani 11/25/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
LotA-1
Special ApplicationsHeLa Whole cell lysate, Antibody dilution used is 1:500 overnight 4 degree
FileView PDF

Publications for USP22 Antibody (NBP1-49644) (1)

Read what people are writing who have used USP22 Antibody (NBP1-49644).

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: WB, IHC-P.

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Product General Protocols

View specific protocols for USP22 Antibody (NBP1-49644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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FAQs for USP22 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)