UCHL1 Antibody (NB300-675)

UCHL1 Antibody

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UCHL1 Antibody Summary:
Species:Bv, Eq, GP, Hu, Mk, Mu, Po, Rt
Applications:IHC-P, WB
Clonality:Polyclonal
Gene:UCHL1
Purity:Affinity purified
Host:Rabbit
Specificity:Rabbit Polyclonal to PGP9.5 - Peripheral Nerve Fiber Marker
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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UCHL1 Antibody Details:
Immunogen:A synthetic peptide made to an internal portion of the human UCHL1 protein sequence (between residues 100-200). UniProt P09936
Localization:Cytoplasmic
Marker:Neuronal Marker
Species Reactivity:

This antibody has been successfully tested on human and mouse samples. The epitope has 100% homology with primate and guinea pig, 99% with rat, pig, and horse, and 92% with bovine protein.

Applications:
Uses:This UCHL1 antibody is useful for Western blot and immunohistochemistry-Paraffin. By Western blot a band at ~25 kDa is seen.
Dilutions:Immunohistochemistry-Paraffin 2.5-5.0 ug/ml, Western Blot 0.5-2.0 ug/ml
Unit Size:0.1 ml (also available: 0.025 ml (sample size))
Concentration:1.0 mg/ml
Positive Controls:3 Positive Controls
Conjugated Antibodies:5 Conjugated Antibodies
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Tris-glycine and 150mM NaCl
Preservative:0.05% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: UCHL1
Entrez7345 (Human)
22223 (Mouse)
Swiss ProtP09936 (Human)
 
Background:

UCHL1 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. It is present in neurons and in cells of the diffuse neuroendocrine system. UCHL1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. This enzyme is also known as PGP9.5. Because of its abundance in nerves, it has been widely used as a marker for peripheral nerve fibers.

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Working with UCHL1

UCHL1 Antibody Images (2)
Immunohistochemistry-Paraffin: UCHL1 Antibody [NB300-675] - Staining of neurons and their cell processes using UCHL1 antibody NB300-675 at 2.5ug/ml. Human brain, Substantia Nigra, Pigmented Neuron, 40X.Western Blot: UCHL1 Antibody [NB300-675] - Detection of UCHL1 in mouse brain lysate using UCHL1 antibody NB 300-675. ECL detection, 60 seconds.
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Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Conjugated Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
UCHL1 Antibody [Biotin]NB300-675BBv, Eq, GP, Hu, Mk, Mu, Po, RtIHC, WB

UCHL1 Antibody [HRP]NB300-675HBv, Eq, GP, Hu, Mk, Mu, Po, RtIHC, WB

UCHL1 Antibody [DyLight 488]NB300-675GBv, Eq, GP, Hu, Mk, Mu, Po, RtIHC, WB

UCHL1 Antibody [DyLight 550]NB300-675RHu, MuIHC, WB

UCHL1 Antibody [DyLight 650]NB300-675CHu, MuIHC, WB

Positive Controls
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Whole Normal Brain LysateNB820-59657MuIP, WB(2)

UCHL1 LysateNBL1-17575WB(1)

Other Positive Controls
  • Mouse brain lysate
Lysates (2)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
UCHL1 LysateNBL1-17575WB(1)

UCHL1 LysateH00007345-T02WB

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
UCHL1 Partial Recombinant ProteinH00007345-Q02HuELISA, WB(1)

UCHL1 Partial Recombinant ProteinH00007345-Q01HuELISA, WB(1)

UCHL1 Recombinant ProteinH00007345-P01HuELISA, WB(1)

Primary Antibodies (38)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
UCHL1 AntibodyNB300-676Eq, Hu, Mk, Mu, Po, RtIHC, WB(2)

UCHL1 AntibodyNB100-65827Bv, Ca, Fe, GP, Hu, Mu, Po, Rb, Rt, ShIF, IHC-P, WB(1)(6)

UCHL1 AntibodyNBP1-96612Hu, Mu, RtFACS, IHC-P, IP, WB(2)

UCHL1 Antibody (EPR4117)NBP1-95538Hu, Mu, RtICC, IHC-P, IP, WB(2)

UCHL1 AntibodyNBP1-87334HuIF, IHC-P, WB(3)

... and 33 more. See All »
RNAi (1)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
UCHL1 RNAiH00007345-R01Hu(1)(4)

Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

Rabbit IgG Antibody [FITC]NB730-FRbICC, IHC-P(1)

Rabbit IgG Antibody [Biotin]NB730-BRbELISA, ICC, IHC-P, WB

Rabbit IgG, H/L Chains Antibody [DyLight 488]NBP1-72944RbFLISA, IF, WB(3)

Rabbit IgG Antibody [Alkaline Phosphatase]NB730-APRbELISA, ICC, IHC-P, WB(1)

See All »

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UCHL1 in Context


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