Tubulin Antibody (YL1/2) 0.1 ml Microtubule Marker

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Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - WB analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2).
Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of Tubulin expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.
Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.
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Ordering Information: NB600-506

  • Catalog Number
    NB600-506
  • Displayed Format
    Unconjugated
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Tubulin Antibody (YL1/2) Summary

Species Human, Mouse, Rat, Avian, Drosophila, Mammal, Primate, Yeast
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Clonality
Monoclonal
Host
Rat
Gene
TUBA1A
Purity
Protein G purified
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Tubulin Antibody (YL1/2) Details

Clone
YL1/2
Isotype
IgG2a
Marker
Microtubule Marker
Epitope
A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Immunogen
Full length native protein (purified) (S. cerevisiae).

Species Reactivity

Human, mouse, rat, monkey, S. cerevisiae, S. pombe, Slime moulds, Allium and birds. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759) Other species have not been tested.
Reviewed Species Read 3 rated 5
using NB600-506 in the following species:

Publications Read Publications using NB600-506 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:10000
  • ELISA 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
Application Notes
This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB600-506AF405
Alexa Fluor (R) 488 Labeled NB600-506AF488
Alexa Fluor (R) 647 Labeled NB600-506AF647
Alexa Fluor (R) 700 Labeled NB600-506AF700
Allophycocyanin Labeled NB600-506APC
DyLight 350 Labeled NB600-506UV
DyLight 405 Labeled NB600-506V
DyLight 405LS Labeled NB600-506V3
DyLight 488 Labeled NB600-506G
DyLight 680 Labeled NB600-506FR
DyLight 755 Labeled NB600-506IR
FITC Labeled NB600-506F
PE Labeled NB600-506PE
PerCP Labeled NB600-506PCP
Reviewed Applications
Read 3 Review rated 5
using
NB600-506 in the following applications:

Publications
Read Publications using
NB600-506 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 5
Recent Reviews
ICC Tested In Human
Reviewed On: 06/11/2014
Rated: 5

Tubulin NB600-506
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Publications

Tubulin Antibody (NB600-506) has been mentioned in at least 14 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150 mM NaCl
Unit Size
0.1 ml
Concentration
1.0 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol TUBA1A

Alternate Names for Tubulin Antibody (YL1/2)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific
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Related Products by Gene

Background

Tubulin alpha-1A chain (TUBA1A), also known as alpha-tubulin 3, tubulin B-alpha-1, B-ALPHA-1, tubulin alpha-3 chain, TUBA3, and LIS3, is a member of the tubulin family. Tubulin is the primary constituent of microtubules. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. The TUBA1A gene is one of three alpha tubulin genes located on chromosome 12q. TUBA1A is found on the cytoskeleton and is expressed predominantly in morphologically differentiated neurologic cells. Mutations to the TUBA1A gene cause a neurological condition called lissencephaly type 3 (LIS3). As a result of defective neuronal migration, LIS3 is characterized by microcephaly, mental retardation, and early-onset epilepsy (PMID: 22264709). The tubulin clone (YL1/2) specifically binds tyrosylated Tubulin (Tyr-Tubulin) (PMID: 6415068).

Reviews for Tubulin Antibody (NB600-506) (3) 53

Read what people are saying who have used Tubulin Antibody (NB600-506).

We have 2 reviews tested in 1 species: Human.
We have 3 reviews tested in 3: Immunocytochemistry, Western Blot, Immunofluorescence.
Average Rating: 5
(Based on 3 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry Tubulin NB600-506
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Excellent
ICC Human Anony- mous 06/11/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Western Blot Tubulin NB600-506
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Excellent
WB Human Anony- mous 06/11/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, COS and NIH/3T3 cells using Tubulin antibody (NB600-506, 1:100).
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Immunofluorescence Tubulin NB600-506
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Excellent
IF Marissa Madden 04/28/2014
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Summary

ApplicationImmunofluorescence

Publications for Tubulin Antibody (NB600-506) (14)

Read what people are writing who have used Tubulin Antibody (NB600-506).

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 2 applications: WB, ICC/IF.

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Filter By Application
WB
(9)
ICC/IF
(4)
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Human
(5)
Mouse
(3)
Rat
(4)
Drosophila
(1)
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Publication Applications Species
Ganem NJ et al. A mechanism linking extra centrosomes to chromosomal instability. Nature;460(7252):278-82. 2009 Jul 9. [PMID: 19506557] (Rat) Rat
Breitling, F et al. A surface expression vector for antibody screening. Gene 104: 147-53. 1991 [PMID: 1916287]
1 Allen JD et al. Extensive contribution of the multidrug transporters P-glycoprotein Mrp1 to basal drug resistance. Cancer Res, 60: 5761-6. 2000 [PMID: 11059771] (WB, Mouse) WB Mouse
Iida, S et al. Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression G1 tetraploidy checkpoint function. Oncogene 23: 5266-5274. 2004 [PMID: 15122335] (WB, Rat) WB Rat
Liang, X-J et al. Endocytic recyling compartments altered in cisplatin-resistant cancer cells. Cancer Res 66: 2346-2353. 2006 [PMID: 16489040] (WB, ICC/IF, Human) ICC/IF, WB Human
Bhattacharya R, Gonzalez AM, DeBiase PJ et al. Recruitment of vimentin to the cell surface by {beta}3 integrin plectin mediates adhesion strength. J Cell Sci;122(9):1390-1400. 2009 [PMID: 19366731] (WB, Rat) WB Rat
O'Connell CB, Loncarek J, Kalab P et al. Relative contributions of chromatin and kinetochores to mitotic spindle assembly. J Cell Biol;187(1):43-51. 2009 [PMID: 19805628] (ICC/IF, Rat) ICC/IF Rat
Batista-Nascimento L, Toledano MB, Thiele DJ et al. Yeast protective response to arsenate involves the repression of the high affinity iron uptake system Biochim Biophys Acta 2013 Jan 4 [PMID: 23295455] (WB, Mouse, Human) WB Mouse, Human
Yang Z, Kenny AE, Brito DA, Rieder CL. Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments. J Cell Biol 2009 Sep 7 [PMID: 19720871] (ICC/IF, Human) ICC/IF Human
Sung M, Giannakakou P. BRCA1 regulates microtubule dynamics and taxane-induced apoptotic cell signaling Oncogene 2013 Mar 25 [PMID: 23524581] (ICC/IF, WB, Human) ICC/IF, WB Human
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. A Meiosis-Specific Form of the APC/C Promotes the Oocyte-to-Embryo Transition by Decreasing Levels of the Polo Kinase Inhibitor Matrimony. PLoS Biol. 2013 Sep [PMID: 24019759] (WB, Drosophila) WB Drosophila
Ganem NJ, Cornils H, Chiu SY et al. Cytokinesis failure triggers hippo tumor suppressor pathway activation. Cell. 2014 Aug 14 [PMID: 25126788] (WB, Human) WB Human
Giron-Martinez A, Perez-Rial S, Terron-Exposito R et al. Proliferative Activity of Liver Growth Factor is Associated with an Improvement of Cigarette Smoke-Induced Emphysema in Mice. PLoS OnE. 2014 Nov 18 [PMID: 25401951] (WB, Mouse) WB Mouse
Hu S, Lu Y, Orr B et al. Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown. Mol. Cancer Ther. 2015 Aug 24 [PMID: 26304236]

Product General Protocols

View specific protocols for Tubulin Antibody (NB600-506): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Tubulin (3)

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Bioinformatics Tool for Tubulin

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Research Areas for Tubulin

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PTMs for Tubulin

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Blogs on Tubulin

Check out the latest blog posts on Tubulin.
The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Who Needs Antibodies for Tubulin Modifications?
Tubulin is a heterodimeric complex composed of alpha and beta subunits. Just like histones, tubulin can undergo post-translational modification, to affect cellular function. Novus Biologicals offers a large database of modified Tubulin antibody produc...  Read full blog post.