The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The antibody does not cross-react with nebulin. Likewise, it does not react with smooth muscle, non-muscle tissues, or cultured cells.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Full length native protein (purified) (Chicken breast muscle).
Species Reactivity:
Cross-reacts with Chicken (Not gizzard smooth muscle), Fish, Human, Pig, Rabbit, Rat and a wide range of other species. Does not cross-react with Cow or Mouse. Not yet tested in other species.
Applications:
Uses:
IF: Use frozen tissue sections of animal skeletal muscle. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Dilutions:
immunofluorescence 1/1000,
Western Blot ,
Immunohistochemistry-Frozen
Unit Size:
0.1 ml
Concentration:
3.5 mg/ml
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
0.01M Na phosphate and 0.15M NaCl (pH7.4)
Preservative:
0.1% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors.By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.