Titin Antibody (NB600-1206)

Titin Antibody (T11)

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Titin Antibody (T11) Summary:
Species:Bv(-), Ch, Fi, Hu, Mu(-), Po, Rb, Rt
Applications:IF, IHC-Fr, WB
Clonality:Monoclonal
Gene:TTN
Purity:IgG purified
Host:Mouse
Specificity:The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The antibody does not cross-react with nebulin. Likewise, it does not react with smooth muscle, non-muscle tissues, or cultured cells.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Titin Antibody (T11) Details:
Clone:T11
Isotype:IgG2b
Immunogen:Full length native protein (purified) (Chicken breast muscle).
Species Reactivity:

Cross-reacts with Chicken (Not gizzard smooth muscle), Fish, Human, Pig, Rabbit, Rat and a wide range of other species. Does not cross-react with Cow or Mouse. Not yet tested in other species.

Applications:
Uses:IF: Use frozen tissue sections of animal skeletal muscle. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Dilutions:immunofluorescence 1/1000, Western Blot , Immunohistochemistry-Frozen
Unit Size:0.1 ml
Concentration:3.5 mg/ml
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:0.01M Na phosphate and 0.15M NaCl (pH7.4)
Preservative:0.1% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: TTN
Entrez7273 (Human)
22138 (Mouse)
84015 (Rat)
 
Background:

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors.By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.

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Publications (1)

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Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Titin in Context


Research Areas related to Titin (2):
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