Thrombospondin Antibody (A4.1) 0.5 ml

Thrombospondin NB600-554

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Ordering Information: NB600-554

Catalog NumberNB600-554
  • FormUnconjugated
  • Size(s)0.5 ml
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Thrombospondin Antibody (A4.1) Summary

 
Species Human, Mouse, Rat, Bovine, Chicken
Tested Applications WB, FLOW, ICC/IF
Clonality Monoclonal
Host Mouse
Gene THBS1
Purity Affinity purified
Specificity It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
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Thrombospondin Antibody (A4.1) Details

 
Clone A4.1
Isotype IgM
Epitope N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Immunogen Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.
Localization Cell Membrane

Species Reactivity

 
Cross-reacts with Human, Rat, Mouse, Cow and Chicken. Not yet tested in other species.

Applications/Dilutions

 
Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes

This antibody can be used in Flow Cytometry, Immunofluorescnece, and Western Blot. By Western blot, this antibody detects a band of ~ 450 kDa(nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets. *Optimal dilutions should be determined by the end user.

Positive Controls

1 Positive Control

Product PDFs

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Protocols and FAQs

Controls & Support Products

Research Tools

View all Thrombospondin Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C. Do not freeze.
Buffer 10mM PBS [pH7.4] with 0.2%BSA
Unit Size 0.5 ml
Concentration 0.2 mg/ml
Preservative 0.09% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol THBS1
Entrez

Alternate Names for Thrombospondin

 
  • thrombospondin-1p180
  • THBS
  • TSP1thrombospondin-1
  • TSPTSP-1
  • THBS-1
  • thrombospondin 1

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Background

 
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.