Thrombospondin-1 Antibody (A4.1) 0.5 ml

Click image to view larger
Western Blot: Thrombospondin Antibody (A4.1) [NB600-554] - Western Blot of TSP from Human Cells in a Non-reducing Gel.
Download Datasheet
See All Related

Ordering Information: NB600-554

  • Catalog Number
    NB600-554
  • Displayed Format
    Unconjugated
  • Size(s)
    0.5 ml
  • Availability
  • Price
    Shipping
  

Thrombospondin-1 Antibody (A4.1) Summary

Species Human, Mouse, Rat, Bovine, Chicken
Tested Applications WB, FLOW, ICC/IF
Clonality
Monoclonal
Host
Mouse
Gene
THBS1
Purity
Affinity purified
Specificity
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Thrombospondin-1 Antibody (A4.1) Details

Clone
A4.1
Isotype
IgM
Epitope
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Immunogen
Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.
Localization
Cell Membrane

Species Reactivity

Not yet tested in other species.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This antibody can be used in Flow Cytometry, Immunofluorescnece, and Western Blot. By Western blot, this antibody detects a band of ~ 450 kDa(nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets. *Optimal dilutions should be determined by the end user.
Positive Controls

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Earn rewards points by submitting your review for Thrombospondin-1 Antibody (NB600-554). Be the first to review this product and earn double rewards points!

Publications

Earn rewards if you have published using Thrombospondin-1 Antibody (NB600-554).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS [pH7.4] with 0.2%BSA
Unit Size
0.5 ml
Concentration
0.2 mg/ml
Preservative
0.09% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol THBS1
Entrez

Alternate Names for Thrombospondin-1 Antibody (A4.1)

  • THBS
  • THBS-1
  • thrombospondin 1
  • thrombospondin-1p180
  • TSP1thrombospondin-1
  • TSPTSP-1
Show More

Related Products by Gene

Background

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Reviews for Thrombospondin-1 Antibody (NB600-554) (0)

There are no reviews for Thrombospondin-1 Antibody (NB600-554).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used Thrombospondin-1 Antibody (NB600-554)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for Thrombospondin-1 Antibody (NB600-554) (0)

There are no publications for Thrombospondin-1 Antibody (NB600-554).
By submitting a publication earn 200 points for your submission points towards our Rewards Program.
Have you published using Thrombospondin-1 Antibody (NB600-554)? Earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for Thrombospondin-1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NB100-664
Western Blot: VEGF Antibody (VG1) [NB100-664] - HUVEC and 293T Cells tested at 1:1000. Image provided by verified customer review.Immunohistochemistry-Paraffin: VEGF Antibody (VG1) [NB100-664] - Analysis of Vascular Endothelial GF formalin-fixed paraffin embedded human angiosarcoma.Western Blot: VEGF Antibody (VG1) [NB100-664] - Western blot analysis of VEGF in human kidney protein using NB100-664.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications IHC-P, IHC-Fr, IHC...MORE

     1 Review

22 Publications
24420002
Immunohistochemistry: Thrombospondin 2 Antibody [24420002] - Most of the normal tissues displayed moderate cytoplasmic positivity. Lymphoid tissues were weakly stained. Malignant tissues exhibited weak to moderate cytoplasmic immunoreactivity. Single cases of colo-rectal and hepatocellular carcinoma showed strong staining. A few cases of lymphomas, endometrial and cervical cancers were negative.  Image and statement courtesy of the Human Protein Atlas (HPA).ELISA: Thrombospondin 2 Antibody [24420002]

Rabbit Polyclonal
Species Human
Applications ELISA, IHC, IHC-P

1 Publication
NB500-390
Flow Cytometry: CD47 Antibody (MEM-122) [NB500-390] - Fig. 1. Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC).  Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03)  Panel B, C, D - porcine PBMC sample stained with anti-CD47 (MEM-122)  Cells in the granulocyte gate were used for analysis.

Mouse Monoclonal
Species Human, Porcine, Primate
Applications WB, FLOW, IHC...MORE

     1 Review

NB400-144
Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug).  Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.Immunocytochemistry/Immunofluorescence: CD36 Antibody [NB400-144] - CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of human adipocytes

Rabbit Polyclonal
Species Human, Mouse, Bovine...MORE
Applications IHC-P, IHC-Fr, IHC...MORE

     1 Review

23 Publications
NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry: Fibronectin Antibody [NBP1-91258] - IHC analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.

Rabbit Polyclonal
Species Human, Mouse
Applications IHC-P, IHC, ICC/IF...MORE

3 Publications
NB600-1536
Immunohistochemistry: FGF-2 Antibody [NB600-1536] - [NB600-1356] - Immunoperoxidase staining (PAP) of U87MG glioma cell suspension implanted into nude rat brain (Han rnu/rnu) using Rabbit Anti-Fibroblast Growth Factor-Basic (NB600-1536). High bFGF expression is exclusively shown within tumor endothelial cells. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without any enzymatic or antigen-demasking pre-processing. From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany. Ref: Stan, A.C., et al., J. Neurosurg., 82, 1044 (1995).Immunohistochemistry: FGF-2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a fibrillary differentiated astrocytoma (WHO grade I, SAMS grade 1) using Rabbit Anti-Fibroblast Growth Factor-Basic. High bFGF expression is shown within tumor cells as well as within endothelial cells .Immunohistochemistry: FGF-2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a gemistocytic differentiated area of an anaplastic astrocytoma (WHO grade III, SAMS grade 3) using Rabbit Anti-Fibroblast Growth Factor-Basic. High bFGF expression is shown within tumor cells as well as within the endothelial cells.

Rabbit Polyclonal
Species Human, Rat, Bovine...MORE
Applications WB, ELISA, IHC...MORE

2 Publications
NB100-57386
Western Blot: p53 Antibody (H53C2) [NB100-57386] - p53 in carcinoma cell extract. Image from verified customer review.

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

     1 Review

NBP2-31143
Western Blot: CTLA4 Antibody (37B3D8.2F2) [NBP2-31143] - WB analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on © partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.

Mouse Monoclonal
Species Human, Mouse
Applications WB

NBP1-19532
Immunocytochemistry/Immunofluorescence: TNF alpha Antibody [NBP1-19532] - TNF alpha antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Immunohistochemistry-Paraffin: TNF alpha Antibody [NBP1-19532] - Immunohistochemical staining of TNF alpha in mouse intestine.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications IHC-P, IHC, ICC/IF...MORE

     1 Review

4 Publications
NB100-65423
Flow Cytometry: HLA DQ/DR Antibody (49.1) [NB100-65423] - Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE MHC CLASS II DQ/DR (POLYMORPHIC).

Mouse Monoclonal
Species Human, Bovine, Goat...MORE
Applications FLOW, IHC, IHC-Fr...MORE

NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of endogenous NOD2 in 50ug of HT29 cell lysate using NB 100-524. ECL detection in 15 minutes.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review. Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 100-524. ECL detection in 10 seconds.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

     1 Review

12 Publications
NB600-586
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Von Willebrand Factor antibody[NB600-586]Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.

Rabbit Polyclonal
Species Human
Applications IHC-P, IHC-Fr, IHC...MORE

1 Publication
NB100-91995
Western Blot: TGF beta 1 Antibody [NB100-91995] - Lane1:Hela cell lysate. Lane2:SP2/0 cell lysate.  Lane3:H9C2 cell lysate.  Lane4:MCF-7 cell lysate.Immunohistochemistry-Paraffin: TGF beta 1 Antibody [NB100-91995] - Paraffin-embedded human breast carcinoma tissue.Western Blot: TGF beta 1 [p Tyr369] Antibody [NB100-91995] - Western blot (WB) analysis of TGF beta-1 (Y369) pAb in extracts from HepG2 cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications IP, IHC-P, IHC...MORE

3 Publications
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Mouse
Applications IHC-P, IHC-Fr, IHC...MORE

28 Publications
NB600-1131
Western Blot: IL6 Antibody [NB600-1131] - Shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax ™ chemiluminescent substrate.Immunocytochemistry/Immunofluorescence: IL6 Antibody [NB600-1131] - IL-6 antibody was tested in Raw264.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: IL6 Antibody [NB600-1131] - IHC staining of IL6 in human bladder cancer using DAB with hematoxylin counterstain.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications IP, IHC-P, IHC...MORE

6 Publications
NB100-724
Immunocytochemistry/Immunofluorescence: CTGF Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NB600-562
Western Blot: CD31/PECAM1 Antibody (JC/70A) [NB600-562] - Western blot analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.Immunocytochemistry/Immunofluorescence: CD31/PECAM1 Antibody (JC/70A) [NB600-562] - The CD31/PECAM antibody was tested in Huvec cells at a 1:10 dilution against Dylight 488 (Green).  Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.  Immunohistochemistry-Frozen: CD31/PECAM1 Antibody (JC/70A) [NB600-562] - Rabbit Heart, CD31 Stained Red. Image from verified customer review.

Mouse Monoclonal
Species Human, Rabbit
Applications IHC-P, IHC-Fr, IHC...MORE

     1 Review

11 Publications
NBP2-27371
Western Blot: SPARC Antibody [NBP2-27371] - analysis of human A375 lysate probled with SPARC antibody at 2 ug/ml. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27371] - Formalin-fixed, paraffin-embedded human placenta stained with SPARC antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Rabbit Polyclonal
Species Human, Primate
Applications WB, IHC-P

Bioinformatics Tool for Thrombospondin-1

Discover related pathways, diseases and genes to Thrombospondin-1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Research Areas for Thrombospondin-1

Find related products by research area.

PTMs for Thrombospondin-1

Learn more about PTMs related to Thrombospondin-1.

Blogs on Thrombospondin-1

There are no specific blogs for Thrombospondin-1, but you can read our latest blog posts.