Thrombospondin-1 Antibody (A4.1) 0.5 ml

Click image to view larger
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of HUVEC Cells.
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of Human Cells in a Non-reducing Gel.
Download Datasheet
See All Related

Ordering Information: NB600-554

  • Catalog Number
    NB600-554
  • Displayed Format
    Unconjugated
  • Size(s)
    0.5 ml
  • Availability
  • Price
    Shipping
  

Thrombospondin-1 Antibody (A4.1) Summary

Species Human, Mouse, Rat, Bovine, Chicken
Tested Applications WB, Flow, ICC/IF
Clonality
Monoclonal
Host
Mouse
Gene
THBS1
Purity
Protein A or G purified
Specificity
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Thrombospondin-1 Antibody (A4.1) Details

Clone
A4.1
Isotype
IgM
Epitope
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Immunogen
Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.
Localization
Cell Membrane

Species Reactivity

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This antibody can be used in Flow Cytometry, Immunofluorescnece, and Western Blot. By Western blot, this antibody detects a band of ~ 450 kDa(nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets. *Optimal dilutions should be determined by the end user.
Positive Controls

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Earn rewards points by submitting your review for Thrombospondin-1 Antibody (NB600-554). Be the first to review this product and earn double rewards points!

Publications

Earn rewards if you have published using Thrombospondin-1 Antibody (NB600-554).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS [pH7.4] with 0.2%BSA
Unit Size
0.5 ml
Concentration
0.2 mg/ml
Preservative
0.09% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol THBS1
Entrez
Uniprot

Alternate Names for Thrombospondin-1 Antibody (A4.1)

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1
Show More

Related Products by Gene

Background

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Reviews for Thrombospondin-1 Antibody (NB600-554) (0)

There are no reviews for Thrombospondin-1 Antibody (NB600-554).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used Thrombospondin-1 Antibody (NB600-554)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for Thrombospondin-1 Antibody (NB600-554) (0)

There are no publications for Thrombospondin-1 Antibody (NB600-554).
By submitting a publication earn 200 points for your submission points towards our Rewards Program.
Have you published using Thrombospondin-1 Antibody (NB600-554)? Earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for Thrombospondin-1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

DVE00


Species Human

278 Publications
DTSP20


Species Human

2 Publications
4670-CD


Species Human
Applications BA, BA2

     1 Review

NB400-144
Western Blot: CD36/SR-B3 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug).  Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody [NB400-144] - CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: CD36/SR-B3 Antibody [NB400-144] - Staining of CD36 in humnan adipocytes.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, ICC/IF, IHC...MORE

     2 Reviews

34 Publications
1918-FN


Species Human
Applications BA

7 Publications
233-FB/CF
Recombinant Human FGF basic (146aa) (Catalog # 233-FB/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is typically 0.1-0.6 ng/mL.1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human
Applications BA

132 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ELISA, Flow...MORE

     2 Reviews

19 Publications
325-CT/CF
Recombinant Human CTLA-4 Fc Chimera (Catalog # 325-CT/CF) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1 Fc Chimera (Catalog # 140-B1) in the presence of PHA.1 μg/lane of Recombinant Human CTLA-4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 51 kDa and 94 kDa, respectively.


Species Human
Applications BA

6 Publications
210-TA/CF
Recombinant Human TNF-alpha  (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.


Species Human
Applications BA

274 Publications
NBP1-84550
Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [KDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.Immunocytochemistry/Immunofluorescence: HLA DQA1 Antibody [NBP1-84550] - Staining of human cell line U-251MG shows positivity in cytoplasm.Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Western Blot Image of anti-NOD2 (2D9) Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-NOD2 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 100-524. ECL detection in 10 seconds.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC...MORE

     1 Review

12 Publications
NB600-586
Immunocytochemistry/Immunofluorescence: Von Willebrand Factor Antibody [NB600-586]Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor. Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

2 Publications
240-B/CF
Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh)TGF-beta 1 (Catalog # 240-B). Control cells were cultured without rhTGF-beta 1. EMT induction was confirmed at 48 h by flow cytometric staining for E-Cadherin (filled; Catalog # FAB18381P), an epithelial cell marker, or an isotype control(Catalog # 240-B). TGF-beta 1 decreased the expression of E-Cadherin.1 ug/lane of Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.Recombinant Human TGF-beta 1 (Catalog # 240-B/CF) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is typically 0.04-0.2 ng/mL.


Species Human
Applications BA

281 Publications
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Mouse
Applications WB, ELISA, ICC/IF...MORE

39 Publications
D6050


Species Human

199 Publications
NB100-724
Immunocytochemistry/Immunofluorescence: CTGF Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

3 Publications
NB100-1642
Flow Cytometry: CD31/PECAM1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM1 (MEC 7.46) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).

Rat Monoclonal
Species Mouse
Applications Flow, ICC/IF, IHC...MORE

16 Publications
DSP00


Species Human

1 Publication

Bioinformatics Tool for Thrombospondin-1

Discover related pathways, diseases and genes to Thrombospondin-1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool
Vizit™, under license from BioVista Inc.

Research Areas for Thrombospondin-1

Find related products by research area.

PTMs for Thrombospondin-1

Learn more about PTMs related to Thrombospondin-1.

Blogs on Thrombospondin-1

Check out the latest blog posts on Thrombospondin-1.
AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.