Thrombospondin-1 Antibody (A4.1) 0.5 ml

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Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of HUVEC Cells.
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of Human Cells in a Non-reducing Gel.
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Ordering Information: NB600-554

  • Catalog Number
    NB600-554
  • Displayed Format
    Unconjugated
  • Size(s)
    0.5 ml
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  • Price
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Thrombospondin-1 Antibody (A4.1) Summary

Species Human, Mouse, Rat, Bovine, Chicken
Tested Applications WB, Flow, ICC/IF
Clonality
Monoclonal
Host
Mouse
Gene
THBS1
Purity
Protein A or G purified
Specificity
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
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Thrombospondin-1 Antibody (A4.1) Details

Clone
A4.1
Isotype
IgM
Epitope
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Immunogen
Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.
Localization
Cell Membrane

Species Reactivity

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This antibody can be used in Flow Cytometry, Immunofluorescnece, and Western Blot. By Western blot, this antibody detects a band of ~ 450 kDa(nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets. *Optimal dilutions should be determined by the end user.
Positive Controls

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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS [pH7.4] with 0.2%BSA
Unit Size
0.5 ml
Concentration
0.2 mg/ml
Preservative
0.09% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol THBS1
Entrez

Alternate Names for Thrombospondin-1 Antibody (A4.1)

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1
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Related Products by Gene

Background

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Thrombospondin-1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Bioinformatics Tool for Thrombospondin-1

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