Telomerase reverse transcriptase Antibody (2C4) (NB100-317)

Telomerase reverse transcriptase Antibody (2C4) (NB100-317) (0.1 ml)

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	Catalog Number	NB100-317 (Sample Size Available)
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anti-hEST2 antibody, anti-TRTEC 2.7.7 antibody, anti-TP2HEST2 antibody, anti-TCS1EC 2.7.7.49 antibody, anti-EST2Telomerase-associated protein 2 antibody, anti-Telomerase catalytic subunit antibody, anti-telomerase reverse transcriptase antibody

Telomerase reverse transcriptase Antibody (2C4) Summary:

Species:	Hu	Gene:	TERT
Applications:	WB, FACS, ICC, IF, IHC-P, IP	Purity:	Ascites
Clonality:	Monoclonal	Host:	Mouse
Specificity:	NB100-317 is specific for hTERT (TERT).
Publications:	NB100-317 has been mentioned in at least 6 Publications

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Telomerase reverse transcriptase Antibody (2C4) Details:

Clone:	2C4
Isotype:	IgM
Immunogen:	Full-length recombinant hTERT from insect cells.
Localization:	Nuclear
Marker:	Embryonic Stem Cell Marker

Species Reactivity:

Human. Other species have not been tested.

Applications:

Uses:	By Western blot, this antibody recognizes a band at ~127 kDa, representing hTERT protein. May see an additional background band at ~110 kDa. Note that the isotype is IgM and the appropriate secondary should be used. This antibody is also useful for immunocytochemistry/immunofluorescence, immunoprecipitation and Flow cytometry/analysis and may also be used for immunohistochemistry of TERT.
Dilutions:	Flow Cytometry 1:50-1:200, Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation, Western Blot 1:500
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	This product is unpurified. Concentration is not relevant.
Positive Controls:	2 Positive Controls

Notes:

Licensed to Novus Biologicals LLC under U.S. Patent No 7,285,639.

Packaging:

Storage:	Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: TERT

Entrez:	7015 (Human)
Uniprot:	O14746 (Human)

Related Genes:

Background:

Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT (TERT) is the rate-limiting catalytic subunit of telomerase. Telomerase is an enzyme uniquely associated with immortal cell lines and is useful for identifying undifferentiated PSCs.

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