TRF2 Antibody 0.1 ml Telomeres marker

TRF2 NB110-57130

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Ordering Information: NB110-57130

Catalog NumberNB110-57130
  • FormUnconjugated
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TRF2 Antibody Summary

 
Species Human, Mouse, Rat, Chinese Hamster, Primate
Tested Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality Polyclonal
Host Rabbit
Gene TERF2
Purity Immunogen affinity purified
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TRF2 Antibody Details

 
Marker Telomeres marker
Immunogen Baculovirus purified TRF2 protein.
Localization Nuclear

Species Reactivity

 
Human, mouse, rat, primate, and Chinese hamster.
Publications Read Publications using NB110-57130 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:2000-1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes

This TRF2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band at ~56 kDa is seen. Immunoprecipitation was reported in scientific literature. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Molecular Weight

56 kDa

Positive Controls

4 Positive Controls

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB110-57130AF405
Alexa Fluor (R) 488 Labeled NB110-57130AF488
Alexa Fluor (R) 647 Labeled NB110-57130AF647
Alexa Fluor (R) 700 Labeled NB110-57130AF700
Biotin Labeled NB110-57130B
DyLight 488 Labeled NB110-57130G
DyLight 550 Labeled NB110-57130R
DyLight 650 Labeled NB110-57130C
HRP Labeled NB110-57130H
Learn about Custom Antibody Labeling
Publications
Read publications using
NB110-57130 in the following applications:
  • WB13publications
  • ChIP3publications
  • ICC/IF9publications
  • IHC1publications
  • IP3publications

Product PDFs

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Publications

TRF2 Antibody (NB110-57130) has been mentioned in at least 33 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all TRF2 Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150mM NaCl
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1.0 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol TERF2
Entrez
Uniprot

Alternate Names for TRF2

 
  • TRBF2
  • telomeric repeat-binding factor 2
  • Telomeric DNA-binding protein
  • TRF2telomeric repeat binding protein 2
  • TTAGGG repeat-binding factor 2
  • telomeric repeat binding factor 2

Related Products by Gene

Background

 
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the telomeric double-stranded 5'-TTAGGG-3' repeat for playing a critical role in the maintenance of telomeres as well as telomere's protection against end-to-end chromosomal fusion, and recruit a number of factors/enzymes required for telomere protection including shelterin complex/telosome (TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1), TERF2IP/RAP1 and DCLRE1B/Apollo. Together with DCLRE1B/Apollo, TRF2 plays a key role in T-loopformation by generating 3' single-stranded overhang at the leading end telomeres and is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage for preventing aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating into repressing homology-directed repair, which can impact telomeres length. Damaged telomeres have been shown to become associated with DNA-damage response factors such as 53BP1, MDC1, gamma-H2AX, ATM and the Mre11/Nbs1/Rad50 or MRN complex, and activate the ATM signaling, leading to cell cycle arrest mediated by the p53/p21 pathway. TRF2 interacts with PNUTS, MCPH1 and DEAD-box RNA helicase DDX39 also for regulating telomere length, telomeric DNA-damage response as well as genome integrity.