TGN46 Antibody (NB110-62093)

TGN46 Antibody

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TGN46 Antibody Summary:
Species:Hu, Mk
Applications:IF, IM, WB
Clonality:Polyclonal
Gene:TGOLN2
Purity:IgG purified
Host:Sheep
Specificity:This is specific for TGN46. TGN46 is a heavily glycosylated protein of around 110-120kDa.
Publications:Antibody has at least 8 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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TGN46 Antibody Details:
Immunogen:Antisera to TGN46 were raised by repeated immunisations of sheep with highly purified recombinant human TGN46.
Marker:Golgi Apparatus Marker
Species Reactivity:

This antibody is reactive against human and primate. Not yet tested in other species. This is the smaller size, 10ug, and a larger size, 25ug, is available at product NB110-40767.

Applications:
Uses:This antibody is useful for western blot, Immunofluorescence, and Immunoelectron microscopy.
Dilutions:immunofluorescence 1:100-1:200, Western Blot 1:500-1:1000, Immunomicroscopy
Unit Size:0.01 mg
Concentration:0.25 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:1% bovine serum albumin
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: TGOLN2
Entrez10618 (Human)
 
Background:

Sheep anti-human TGN46 recognises a glycoprotein designated TGN46 that is localised primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network

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Working with TGN46

TGN46 Antibody Images (2)
Immunofluorescence: TGN46 - Affinity Purified Antibody [NB110-62093] - Immunofluorescence staining of human TGN46 in 3% paraformaldehyde-fixed HeLa cells using affinity purified sheep anti-human TGN46 at 1/100. Secondary antibody was donkey anti-sheep Alexa Fluor 488.20ìg HeLa cell lysate was run on an SDS-PAGE gel and western blotted using affinity purified sheep anti-human TGN46 at 1/1000
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Publications (8)

TGN46 Antibody Background References:

  1. Matas O.B. et al (2004). Association of Cdc42/N-WASP/Arp2/3 Signaling Pathway with Golgi Membranes. Traffic; 5: 838
  2. Vuillier F. et al (2005). Lower levels of surface B-cell-receptor expression in chronic lymphocytic leukemia are associated with glycosylation folding defects of the mu CD79a chains. Blood; 105: 2933-2940

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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Jump to: Novus Explorer, Research Areas

TGN46 in Context


Research Areas related to TGN46 (2):
Novus Explorer for TGN46 gene

Try our bioinformatic tool that shows the relationships between TGOLN2 and other genes, diseases, pathways and post-translational modifications.