Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Antisera to TGN46 were raised by repeated immunisations of sheep with highly purified recombinant human TGN46.
Marker:
Golgi Apparatus Marker
Species Reactivity:
This antibody is reactive against human and primate. Not yet tested in other species.
This is the smaller size, 10ug, and a larger size, 25ug, is available at product NB110-40767.
Applications:
Uses:
This antibody is useful for western blot, Immunofluorescence, and Immunoelectron microscopy.
Dilutions:
immunofluorescence 1:100-1:200,
Western Blot 1:500-1:1000,
Immunomicroscopy
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
1% bovine serum albumin
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Sheep anti-human TGN46 recognises a glycoprotein designated TGN46 that is localised primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network
Immunofluorescence: TGN46 - Affinity Purified Antibody [NB110-62093] - Immunofluorescence staining of human TGN46 in 3% paraformaldehyde-fixed HeLa cells using affinity purified sheep anti-human TGN46 at 1/100. Secondary antibody was donkey anti-sheep Alexa Fluor 488.
20ìg HeLa cell lysate was run on an SDS-PAGE gel and western blotted using affinity purified sheep anti-human TGN46 at 1/1000
Matas O.B. et al (2004). Association of Cdc42/N-WASP/Arp2/3 Signaling Pathway with Golgi Membranes. Traffic; 5: 838
Vuillier F. et al (2005). Lower levels of surface B-cell-receptor expression in chronic lymphocytic leukemia are associated with glycosylation folding defects of the mu CD79a chains. Blood; 105: 2933-2940
Ponnambalam S. et al (1996). Primate homologues of rat TGN38: primary structure, expression functional implications. J Cell Sci; 109: 675-685
Prescott A.R. et al (1997). Distinct compartmentalization of TGN46 beta 1, 4-galactosyltransferase in HeLa cells. Eur J Cell Biol; 72: 238-246
Mills I.G. et al (2005). Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors. J Cell Biol; 70: 191-200
Marullo S. et al (2004). Homodimerization of the {beta}2-Adrenergic Receptor as a Prerequisite for Cell Surface Targeting. J Biol Chem; 279: 33390-33397
Drakesmith H. et al (2005). HIV-1 Nef down-regulates the hemochromatosis protein HFE, manipulating cellular iron homeostasis. PNAS; 102: 11017-11022
Linstedt A.D. et al (2004). A Cycling cis-Golgi Protein Mediates Endosome-to-Golgi Traffic. Mol Biol Cell; 15: 4798-4806