TGF-beta 3 Protein 5 ug

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Ordering Information: NBP1-71646

  • Catalog Number
    NBP1-71646
  • Size(s)
  • Availability


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    TGF-beta 3 Protein Summary

    Tested Applications WB, PAGE
    Gene
    TGFB3
    Guarantee Plus
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    TGF-beta 3 Protein Details

    Epitope
    HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
    Immunogen
    Human recombinant protein expressed in Nicotiana benthamiana. HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS

    Applications/Dilutions

    Dilutions
    • Western Blot
    • SDS-Page
    Application Notes
    Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF beta 3. Biological Activity: The biological activity of TGF-beta 3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 less than 40ng/ml

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    Notes

    Molecular formula: C600 H902 N166 O174 S10 Extinction coefficient: E 0.1% 1.72 (A 280 nm) Molecular weight: recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond p.I: 6.75 Purity: > 97 % as determined by SDS-PAGE gel Endotoxin level* < 0.04 EU/ ìg protein (LAL method). Storage and Stability: It is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.

    Packaging, Storage & Formulations

    Storage
    Store at -80C. Avoid freeze-thaw cycles.
    Formulation
    Lyophilized from a Tris HCl 0.05M buffer at pH 7.4.
    Unit Size
    5 ug
    Concentration
    LYOPH
    Reconstitution Instructions
    Lyophilized protein should be reconstituted in water to a concentration of 50 ng /ul. Due to the protein nature, dimmers and multimers may be observed.
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

    Bioinformatics

    Gene Symbol TGFB3

    Alternate Names for TGF-beta 3 Protein

    • ARVD
    • FLJ16571
    • TGF-beta3
    • TGF-beta-3
    • transforming growth factor beta-3
    • transforming growth factor, beta 3
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    Related Products by Gene

    Background

    Description: Recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. Source: It is produced by transient expression of TGF-beta 3 in non-transgenic plants. Recombinant human TGF-beta 3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product contains no animal derived components or impurities.

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    Product General Protocols

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