TGF beta 3 Protein 5 ug

TGF beta 3 NBP1-71646

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Ordering Information

Catalog Number  NBP1-71646

Availability  Discontinued

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TGF beta 3 Protein Summary

 
Tested Applications WB, PAGE
Gene TGFB3
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TGF beta 3 Protein Details

 
Epitope HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYY ANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPL TILYYVGRTPKVEQLSNMVVKSCKCS
Immunogen Human recombinant protein expressed in Nicotiana benthamiana. HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKG YYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQD LEPLTILYYVGRTPKVEQLSNMVVKSCKCS

Applications/Dilutions

 
Dilutions
  • Western Blot
  • SDS-Page
Application Notes

Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF beta 3. Biological Activity: The biological activity of TGF-beta 3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 less than 40ng/ml

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Notes

 
Molecular formula: C600 H902 N166 O174 S10 Extinction coefficient: E 0.1% 1.72 (A 280 nm) Molecular weight: recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond p.I: 6.75 Purity: > 97 % as determined by SDS-PAGE gel Endotoxin level* < 0.04 EU/ ?g protein (LAL method). Storage and Stability: It is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.

Packaging, Storage & Formulations

 
Storage Store at -80C. Avoid freeze-thaw cycles.
Formulation Lyophilized from a Tris HCl 0.05M buffer at pH 7.4.
Unit Size 5 ug
Concentration Lyoph
Reconstitution Instructions Lyophilized protein should be reconstituted in water to a concentration of 50 ng /ul. Due to the protein nature, dimmers and multimers may be observed.
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Bioinformatics

 
Gene Symbol TGFB3

Alternate Names for TGF beta 3

 
  • transforming growth factor beta-3
  • TGF-beta3
  • FLJ16571
  • ARVD
  • TGF-beta-3
  • transforming growth factor, beta 3

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Background

 
Description: Recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. Source: It is produced by transient expression of TGF-beta 3 in non-transgenic plants. Recombinant human TGF-beta 3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product contains no animal derived components or impurities.