Jump to Information on TGF beta 3 Protein (NBP1-71646)
TGF beta 3 Protein Summary
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TGF beta 3 Protein Details
||HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYY ANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPL TILYYVGRTPKVEQLSNMVVKSCKCS
||Human recombinant protein expressed in Nicotiana benthamiana. HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKG YYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQD LEPLTILYYVGRTPKVEQLSNMVVKSCKCS
Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF beta 3. Biological Activity: The biological activity of TGF-beta 3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 less than 40ng/ml
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Controls & Support Products
Molecular formula: C600 H902 N166 O174 S10 Extinction coefficient: E 0.1% 1.72 (A 280 nm) Molecular weight: recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond p.I: 6.75 Purity: > 97 % as determined by SDS-PAGE gel Endotoxin level* < 0.04 EU/ ?g protein (LAL method). Storage and Stability: It is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.
Packaging, Storage & Formulations
||Store at -80C. Avoid freeze-thaw cycles.
||Lyophilized from a Tris HCl 0.05M buffer at pH 7.4.
Lyophilized protein should be reconstituted in water to a concentration of 50 ng /ul. Due to the protein nature, dimmers and multimers may be observed.
||This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
Alternate Names for TGF beta 3
- transforming growth factor beta-3
- transforming growth factor, beta 3
Related Products by Gene
, TRANSFORMING GROWTH FACTOR-BETA TYPE II RECEPTOR
Description: Recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.
Source: It is produced by transient expression of TGF-beta 3 in non-transgenic plants. Recombinant human TGF-beta 3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product contains no animal derived components or impurities.