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Survivin Antibody (60.11) (NB500-238) (0.1 ml)

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Catalog NumberNB500-238 ( Sample Size Available )
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View All Survivin Primary Antibodies
(see alternate names)
anti-Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5 antibody, anti-API4 survivin variant 3 alpha antibody, anti-baculoviral IAP repeat-containing 5 antibody, anti-IAP4 antibody, anti-BIRC5 antibody, anti-EPR-1 antibody, anti-Apoptosis inhibitor survivin antibody
Survivin Antibody (60.11) Summary:
Species: Hu, Mu, Rt Gene: BIRC5
Applications: WB, ICC/IF, IHC, IHC-P Purity: Protein G purified
Clonality: Monoclonal Host: Mouse
Specificity: NB500-238 is specific for the cytoplasmic form of Survivin.
Publications: NB500-238 has been mentioned in at least 20 Publications
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Survivin Antibody (60.11) Details:
Clone: 60.11
Isotype: IgG2a Kappa
Immunogen: Full length recombinant human Survivin [UniProt# O15392]
Localization: Cytoplasmic
Marker: Astrocyte Marker
Epitope: The epitope recognized is between amino acids 27-67
  Species Reactivity:
Human, rat and mouse.
  Applications:
Uses: This Survivin (60.11) antibody is useful for Immunocytochemistry (PMID: 21976248) /Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin.
Dilutions: Immunocytochemistry/Immunofluorescence 1:50-1:200
Immunohistochemistry 1:50-1:200
Immunohistochemistry-Paraffin 1:50-1:200
Western Blot 1:1000
MW : 16.5 kDa
Unit Size: 0.1 ml (also available: 0.025 ml (sample size) )
Concentration: 0.9 mg/ml
Positive Controls: 2 Positive Controls
Conjugated Antibodies: 5 Conjugated Antibodies
Packaging:
Storage: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer: PBS
Preservative: 0.1% Sodium Azide
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
 
Gene Symbol: BIRC5
Entrez 332 (Human)
11799 (Mouse)
64041 (Rat)
Uniprot O15392 (Human)
Related Genes
Background:

Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.

Images (4)
Western Blot: Survivin Antibody (60.11) [NB500-238] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Survivin. Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [NB500-238] - Detection of Survivin protein (Green) in Hela cells using NB500-238. Nuclei(Blue) were counterstained using Hoechst 33258.
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-238] - Staining of ovary cancer. Western Blot: Survivin Antibody (60.11) [NB500-238] - Survivin detection in 30ug of HeLa whole cell extract using NB500-238 at 1ug/ml.
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Patrick Hsieh on 12/8/2011
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Related Products (690)
2 Positive Controls
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50 Primary Antibodies
4 RNAi
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Exploring Survivin
14   Related Research Areas
 
Working with Survivin
20   Publications
1   Reviews from researchers like you
1   Protocols and Procedures
Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
Working with Survivin  

Survivin Antibody (60.11) Images (4)

Western Blot: Survivin Antibody (60.11) [NB500-238] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Survivin.
 

(view larger) 		Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [NB500-238] - Detection of Survivin protein (Green) in Hela cells using NB500-238. Nuclei(Blue) were counterstained using Hoechst 33258.
 
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-238] - Staining of ovary cancer.
 
Western Blot: Survivin Antibody (60.11) [NB500-238] - Survivin detection in 30ug of HeLa whole cell extract using NB500-238 at 1ug/ml.
 
 

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Application: Western Blot
Sample Tested: Mouse heart
Sample Pretreatment: Heart lysate
Species: Mouse
Blocking Details: 5% BSA/TBST
Dilution Ratio: 1:1000
Secondary Description: Goat anti-mouse
Detection Notes: ECL
 

Publications (20)

 
Survivin Antibody (60.11) Product Specific:
  1. Rho JH, Lampe PD. High-Throughput Screening for Native Autoantigen-Autoantibody Complexes Using Antibody Microarrays J Proteome Res 2013 Mar 29 [PMID: 23541305] (Human)
  2. Khan S, Jutzy JM, Valenzuela MM et al Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer PLoS One 2012 [PMID: 23091600] (WB, Human)

more...

  1. Keerthivasan G, Liu H, Gump JM et al A novel role for survivin in erythroblast enucleation Haematologica 2012 Oct [PMID: 22491741] (WB, ICC/IF, Mouse)
  2. Zhang H, Wang Y, Liu C et al. DNA and adenovirus tumor vaccine expressing truncated survivin generates specific immune responses and anti-tumor effects in a murine melanoma model Cancer Immunol Immunother 2012 Jun 17 [PMID: 22706381] (WB, Human)
  3. Lopergolo A, Tavecchio M, Lisanti S et al. Chk2 Phosphorylation of Survivin-{Delta}Ex3 Contributes to a DNA Damage-Sensing Checkpoint in Cancer Cancer Res 2012 Jul 1 [PMID: 22586065] (IHC, Human)
  4. Schöneberger H, Weiss A, Brill B, et al. The integration of a Stat3 specific peptide aptamer into the thioredoxin scaffold protein strongly enhances its inhibitory potency. Hormone Molecular Biology and Clinical Investigation 135(1):1-9. 2011 Jun.
  5. Lechler P, Wu X, Bernhardt W, et al. The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney. Am J Pathol171(5):1483-98. 2007 Nov. [PMID: 17982126]
  6. Koyama S. Differential expression of intracellular apoptotic signaling molecules in tumor and tumor-infiltrating lymphocytes during development of invasion and/or metastasis of gastric carcinoma. Dig Dis Sci48(12):2290-300. 2003 Dec. [PMID: 14714615]
  7. Ku JH, Kwak C, Lee HS, Park HK, Lee E, Lee SE. Expression of survivin, a novel inhibitor of apoptosis, in superficial transitional cell carcinoma of the bladder. J Urol171(2 Pt 1):631-5. 2004 Feb. [PMID: 14713775]
  8. Sakao K, Desineni S, Hahm ER, Singh SV. Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo. Prostate. 2011 Dec 7. [PMID: 22161756] (WB, IHC, Human, Mouse)
  9. Lechler P, Balakrishnan S, Schaumburger J, et al. The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro. BMC Musculoskelet Disord. 2011 Jul 5. [PMID: 21729321]
  10. Lechler P, Renkawitz T, Campean V, et al. The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC Cancer11(1):120. 2011 Apr 2. [PMID: 21457573]
  11. Allen, S, et al. Survivin expression in mouse skin prevents papilloma regression and promotes chemical-induced tumor progression. Cancer Res63(3):567-72. 2003 Feb 1. [PMID: 12566297]
  12. Fortugno, P, et al. Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function. J Cell Sci115(Pt 3):575-85. 2002 Feb 1. [PMID: 11861764]
  13. Femia AP, Salvianti F, Luceri C, et al. Sustained proliferation and resistance to apoptosis after a cytotoxic insult are early alterations in rat colon carcinogenesis. Int J Cancer;131(3):529-36. 2012 Aug 1. [PMID: 21976248] (IHC, Rat)
  14. Lechler P, Schaumburger J, Köck FX, et al. The Oncofetal Gene Survivin Promotes Cell Proliferation and Survival in Primary Human Osteoblastic Cells. Calcif Tissue Int. 2011 Jun 15. [PMID: 21674243] (ICC/IF, WB, Human)
  15. Brocardo M, et al. Mitochondrial targeting of adenomatous polyposis coli protein is stimulated by truncating cancer mutations: regulation of Bcl-2 and implications for cell survival. J Biol Chem283(9):5950-9. 2008 Feb 29. [PMID: 18160396]
  16. Ryan, B, et al. Expression of survivin and its splice variants survivin-2B and survivin-DeltaEx3 in breast cancer. Br J Cancer92(1):120-4. 2005 Jan 17. [PMID: 15611790]
  17. Xu, Z, et al. Promyelocytic leukemia protein 4 induces apoptosis by inhibition of survivin expression. J Biol Chem279(3):1838-44. 2004 Jan 16. [PMID: 14597622]
  18. Zhu, L, et al. Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothelial cells by hypoxic preconditioning. FEBS Lett508(3):369-74. 2001 Nov 23. [PMID: 11728454]

Protocols

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Jump to: ELISA Kits, Conjugated Antibodies, Positive Controls, Antibody Packs, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
  ELISA Kits (1)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin ELISA Kit [Biotin] KA0441 Hu 0 0 0

  Conjugated Antibodies (5)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin Antibody (60.11) [DyLight 650] NB500-238C Hu, Mu, Rt WB, ICC/IF, IHC, IHC-P 0 0 0

Survivin Antibody (60.11) [Biotin] NB500-238B Hu, Mu, Rt WB, ICC/IF, IHC, IHC-P 0 0 0

Survivin Antibody (60.11) [HRP] NB500-238H Hu, Mu, Rt WB, ICC/IF, IHC, IHC-P 0 0 0

Survivin Antibody (60.11) [DyLight 488] NB500-238G Hu, Mu, Rt WB, ICC/IF, IHC, IHC-P 0 0 0

Survivin Antibody (60.11) [DyLight 550] NB500-238R Hu, Mu, Rt WB, ICC/IF, IHC, IHC-P 0 0 0

  Positive Controls (2)
Name Catalog# Reactivity Applications Images Publications Reviews
HeLa Whole Cell Lysate NB800-PC1 WB 2 0 0

Survivin Overexpression Lysate NBL1-07987 WB 1 0 0

  Antibody Packs (1)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin Antibody Pack NB100-911 Hu, Mu, Rt, Ca, Fe WB, ICC/IF, IHC, IHC-P, IP 1 6 0

  Antibody Pairs (1)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin Antibody Pair H00000332-AP11 Hu S-ELISA 1 0 0

  Lysates (2)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin Overexpression Lysate H00000332-T01 Hu WB 2 0 0

Survivin Overexpression Lysate NBL1-07987 WB 1 0 0

  Peptides and Proteins (7)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin Peptide NB110-92717PEP 0 0 0

Survivin [ac Lys129] Peptide NBP1-47639PEP 0 0 0

Survivin Recombinant Protein H00000332-P01 Hu WB, ELISA, PA 1 2 0

Survivin Partial Recombinant Protein H00000332-Q01 Hu WB, ELISA, PA 1 0 0

Survivin Protein NBC1-18508 Hu ELISA, PAGE 1 0 0

... and 2 more. See All »
  Primary Antibodies (50)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin Antibody NB500-201 Hu, Mu, Rt, Ca, Fe, Ha WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP 4 154 7

Survivin Antibody (60.11) NB500-205 Hu, Mu, Rt FLOW, ICC/IF, IP 3 15 0

Survivin [p Thr34] Antibody NB500-236 Hu, Mu, Bv, Ca, Fe, Mk, Po WB, ICC/IF 2 7 1

Survivin Antibody (32.1) NB500-237 Hu, Ha WB, ICC/IF, IHC, IHC-P, IP 1 8 0

Survivin Antibody (8E2) NB500-644 Hu, Rt ICC/IF, IHC, IHC-P 2 14 0

... and 45 more. See All »
  RNAi (4)
Name Catalog# Reactivity Applications Images Publications Reviews
Survivin RNAi H00000332-R01V Hu 2 0 0

Survivin RNAi H00000332-R03 Hu 1 0 0

Survivin RNAi H00000332-R02 Hu 1 0 0

Survivin RNAi H00000332-R01 Hu 1 0 0

  Secondary Antibodies (617)
Name Catalog# Reactivity Applications Images Publications Reviews
Rabbit anti-Mouse IgG Antibody [Alkaline Phosphatase] NB720-AP Mu WB, ELISA, ICC/IF, IHC, IHC-P 0 1 0

Rabbit anti-Mouse IgG Antibody [Biotin] NB720-B Mu WB, ELISA, IHC, IHC-P 0 0 0

Rabbit anti-Mouse IgG Antibody [HRP] NB720-H Mu WB, ELISA, ICC/IF, IHC, IHC-P 1 1 1

Goat anti-Mouse IgG Antibody [FITC] NB720-F Mu ICC/IF, IHC, IHC-P 0 3 0

Goat anti-Mouse IgG Antibody [HRP] NB7570 Mu WB, ELISA, ICC/IF, IHC, IHC-P 0 1 0

See All »
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Research Areas related to Survivin (14):
 
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