Survivin Antibody (60.11) 0.1 ml

Survivin NB500-205

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Ordering Information: NB500-205

Catalog NumberNB500-205
  • FormUnconjugated
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Survivin Antibody (60.11) Summary

 
Species Human, Mouse, Rat
Tested Applications WB, FLOW, ICC/IF, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene BIRC5
Purity Ascites
Specificity NB500-205 is specific for the cytoplasmic form of survivin.
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Survivin Antibody (60.11) Details

 
Clone 60.11
Isotype IgG2a Kappa
Epitope The epitope recognized is between amino acids 57-67.
Immunogen Full length recombinant human Survivin [UniProt# O15392]

Species Reactivity

 
Human, mouse and rat
Publications Read Publications using NB500-205 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot
  • Flow Cytometry 1:200
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes

This Survivin (60.11) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Immunohistochemistry-Paraffin and Western Blot were reported in scientific literature. This antibody weakly detects Survivin in Western blot; for this application we suggest using NB500-235 instead. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 22841823)

Molecular Weight

16.5 kDa

Positive Controls

2 Positive Controls

Publications
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NB500-205 in the following applications:

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Publications

Survivin Antibody (NB500-205) has been mentioned in at least 16 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

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Packaging, Storage & Formulations

 
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration This product is unpurified. The exact concentration of antibody is not quantifiable.
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol BIRC5
Entrez
Uniprot

Alternate Names for Survivin

 
  • BIRC5
  • IAP4
  • EPR-1
  • baculoviral IAP repeat-containing 5
  • Apoptosis inhibitor survivin
  • Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5
  • API4 survivin variant 3 alpha

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Background

 
Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.