Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.
![Western Blot: Survivin Antibody (60.11) [NB500-205] - Survivin detected in rat aorta smooth muscle cell lysate.](http://images.novusbio.com/fullsize2/Survivin Antibody (60.11)-Western Blot-NB500-205-img0007.jpg)
![Flow Cytometry: Survivin Antibody (60.11) [NB500-205] - Staining of NTERA-2 cells using NB500-205 at a 1:2000 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.](http://images.novusbio.com/fullsize2/Survivin Antibody (60.11)-Flow Cytometry-NB500-205-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [NB500-205] - HeLa cells stained NB500-205 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize2/Survivin Antibody (60.11)-Immunocytochemistry-Immunofluorescence-NB500-205-img0006.jpg)
-Western Blot-NB500-210-img0004.jpg "Western Blot: Caspase 3 Antibody (CPP32 4-1-18) [NB500-210] - Western blot detection of Caspase (19 and 35 kDa) from HEK293 cell extract using NB500-210. Lanes 1 and 2 contain inactive and active Caspase, respectively.")
-Immunohistochemistry-Paraffin-NB500-210-img0001.jpg "Immunohistochemistry-Paraffin: Caspase 3 Antibody (CPP32 4-1-18) [NB500-210] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer.")
-Western Blot-NB110-55551-img0004.jpg "Western Blot: Bcl2 Antibody (E17) [NB110-55551] Jurkat cell lysate.")
-Immunohistochemistry-NB110-55551-img0002.jpg "Immunohistochemistry: Bcl2 Antibody (E17) [NB110-55551] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab")
-Western-Blot-NB200-103-img0001.jpg "Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.")
-Immunohistochemistry-Paraffin-NB600-713-img0001.jpg "Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713] - Human pancreas with Mouse anti Human Amylin Peptide, showing labeling of Islet of Langerhans.")
-Western-Blot-NB500-303-img0004.jpg "Western Blot: p21 Antibody (WA-1) [NB500-303] - WB analysis of p21 in horse skin cells. Image courtesy of prodcut review submitted by Karine Deschene.")
-Immunocytochemistry-Immunofluorescence-NB500-303-img0003.jpg "Immunocytochemistry/Immunofluorescence: p21 Antibody (WA-1) [NB500-303] - IF analysis of p21 in horse skin cells. Image courtesy of prodcut review submitted by Karine Deschene.")
-Western Blot-NB100-664-img0002.jpg "Western Blot: VEGF Antibody (VG1) [NB100-664] - Western blot analysis of VEGF in human kidney protein using NB100-664.")
-Western Blot-NB100-79891-img0004.jpg "Western Blot: AKT1 [p Ser473] Antibody (EP2109Y) [NB100-79891] Untreated cervical carcinoma.")
-Immunohistochemistry-NB100-79891-img0002.jpg "Immunohistochemistry: AKT1 [p Ser473] Antibody (EP2109Y) [NB100-79891] - Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using NB100-79891.")
-Immunohistochemistry-Frozen-NBP1-28566-img0002.jpg "Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Of Mouse retina with no pretreatment")
-Western Blot-NB500-213-img0002.jpg "Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.")
-Western Blot-NB110-56948-img0004.jpg "Western Blot: EGFR [p Tyr1173] Antibody (E124) [NB110-56948] - (1) A431 and (2) A431 + EGF cell lysates, 1:1,000 dilution.")
-Immunocytochemistry-Immunofluorescence-NB110-56948-img0005.jpg "Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1173] Antibody (E124) [NB110-56948] Phosphatase-treated A431 cells using Phospho-EGFR.")
-Western Blot-NB110-56901-img0004.jpg "Western Blot: Cyclin D1 Antibody (EP272Y) [NB110-56901] - MCF-7 cell lysate.")
-Immunocytochemistry-Immunofluorescence-NB110-56901-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (EP272Y) [NB110-56901] MCF-7 cells.")
