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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Publications
Read Publication using NB100-78268 in the following applications:
RNA polymerase II elongation factor-like protein OCP2
RNA polymerase II elongation factor-like protein
SKP1
SKP1A
SKP1Acyclin A/CDK2-associated protein p19
S-phase kinase-associated protein 1
TCEB1LSIII
Transcription elongation factor B
Background
SKP1A (S-phase kinase-associated protein 1A) is an 18 kD protein found in the nucleus an the cytoplasm. This protein contains an F-box and has two different isoforms with slightly differnt molecular weights (A, 18.1 kD and B, 18.6 kD). SKP1A functions as a substrate recognition component of SCF ubiquitin ligase complex and RNA polymerase II elongation factor. SKP1A has also been shown to collaborate with a network of proteins to control beta-catenin levels and affect beta-catenin dependent TCF transcription factors. SKP1A can be modified by glycosylation and ubquitination. This protein has been shown to interact with cyclin F, Skp2, CUL1, cyclin A, and CDK2. The Poly6032 antibody recognizes the N-terminal human and mouse SKP1A protein and has been shown to be useful for Western blotting.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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