SUG1 Antibody (25D5) (NB100-345)

SUG1 Antibody (25D5) (NB100-345) (0.1 mg)

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	Catalog Number	NB100-345
	Size(s)	0.1 mg	Price:
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anti-Proteasome 26S subunit ATPase 5 antibody, anti-thyroid receptor interactor 1 antibody, anti-p45 antibody, anti-Tat-binding protein homolog 10 antibody, anti-TBP10 antibody, anti-proteasome 26S ATPase subunit 5 antibody, anti-SUG-1 antibody, anti-MSUG1 protein antibody, anti-S8 antibody, anti-p45/SUG26S protease regulatory subunit 8 antibody, anti-SUG126S proteasome AAA-ATPase subunit RPT6 antibody, anti-TRIP1Thyroid hormone receptor-interacting protein 1 antibody, anti-Proteasome subunit p45 antibody, anti-proteasome (prosome, macropain) 26S subunit, ATPase, 5 antibody

SUG1 Antibody (25D5) Summary:

Species:	Hu	Gene:	PSMC5
Applications:	WB	Purity:	Protein G purified
Clonality:	Monoclonal	Host:	Mouse
Specificity:	SUG1 (25D5)
Publications:	NB100-345 has at least 3 Background References

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Lysates	Peptides and Proteins
Primary Antibodies	RNAi
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SUG1 Antibody (25D5) Details:

Clone:	25D5
Isotype:	IgG
Immunogen:	Recombinant SUG1 purified from E. coli.
Localization:	Cytoplasmic

Species Reactivity:

Human.

Applications:

Uses:	This antibody is useful for Western Blot. ChIP – See customer review.
Dilutions:	Western Blot 1:1000
MW:	45 kDa
Unit Size:	0.1 mg
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	PBS, pH7.4
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: PSMC5

Entrez:	5705 (Human)
Uniprot:	P62195 (Human)

Related Genes:

Background:

Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.

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Reviews (1)

Anonymous on 3/2/2010

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SUG1 Antibody (25D5) Images (1)

	Western Blot: PSMC5 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji

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Reviewed by:
Anonymous 3/2/2010

Application:	ChIP
Sample Tested:	Human Whole Cell lysate
Species:	Human
Positive Control:	IgG binding
ChIP Type:	Cross-linking (X-ChIP)
Results:	It works well with both ChIP and WB. I am happy wit the Ab.

Dilution Ratio:	Undiluted
Incubation Time:	overnight
Incubation Temperature:	4 4
Incubation Dilution Buffer:	ChIP dilution Biffer

Method:

Real-time PCR

Publications (3)

SUG1 Antibody (25D5) Background References:

Lommel, L., et al. The 26S proteasome negatively regulates the level of overall genomic nucleotide excision repair. Nucl. Acid Res. 24:4839, 2000.
Schauber, C., et al. Rad23 links DNA repair to the ubiquitin/proteasome pathway. Nature. 39:715, 1998.

more...

Weeda, G., et al. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteosome putative transcription factor. Nucl. Acid Res. 25:2274, 1997.

Protocols

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RNAi (1)

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