A genomic peptide made to an internal region of the human STK33 (within residues 1-150). [Swiss-Prot Q9BYT3]
Cytoplasm - perinuclear region.
Human and mouse.
Western Blot 1:20000
This STK33 antibody is useful for ICC/IF, IHC and Western blot where a doublet is seen ~57 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
STK33 (serine/threonine-protein kinase 33) belongs to Ca2+/calmodulin-dependent kinase family (CAMK) and mainly play role in the dynamic behavior of intermediate filament cytoskeleton by phosphorylation of vimentin. STK33 undergoes compulsory autophosphorylation and then phosphorylates vimentin in vitro specifically in the vimentin head domain. STK33 and vimentin have been shown to be interacting protein partners in vivo also and STK33 has been proposed to be involved in the dynamics of intermediate filament assembly/disassembly via vimentin phosphorylation. Downregulation of STK33 by RNAi or inhibition of its kinase activity by overexpression of a kinase dead (KD) K145M STK33 protein conferred a lethal phenotype to mutant KRAS cell lines. Suppression of STK33 expression by either method resulted in inhibition of p70S6K and RPS6 phosphorylation but had no effect on the phosphorylation of AKT, ERK1/2, or mTOR, suggesting that STK33 is downstream from these nodes, but upstream from p70S6K.
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FAQs for STK33 (2)
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)