STK33 Antibody 0.025 ml

Click image to view larger
Western Blot: STK33 Antibody [NBP1-54156] - Detection of Stk33 in HEK293 cell lysate.
Immunocytochemistry/Immunofluorescence: STK33 Antibody [NBP1-54156] - ICC staining of STK33 in HEK293 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: STK33 Antibody [NBP1-54156] - IHC staining of STK33 in mouse testis.
Download Datasheet
See All Related

Ordering Information: NBP1-54156

  • Catalog Number
    NBP1-54156
  • Displayed Format
    Unconjugated
  • Other Formats
  • Size(s)
  • Availability
  • Price
    Shipping
  

STK33 Antibody Summary

Species Human, Mouse
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
STK33
Purity
Immunogen affinity purified
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

STK33 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human STK33 (within residues 1-150). [Swiss-Prot Q9BYT3]
Localization
Cytoplasm - perinuclear region.

Species Reactivity

Human and mouse.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This STK33 antibody is useful for ICC/IF, IHC and Western blot where a doublet is seen ~57 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-54156AF405
Alexa Fluor (R) 488 Labeled NBP1-54156AF488
Alexa Fluor (R) 647 Labeled NBP1-54156AF647
Alexa Fluor (R) 700 Labeled NBP1-54156AF700
Biotin Labeled NBP1-54156B
DyLight 405 Labeled NBP1-54156V
DyLight 488 Labeled NBP1-54156G
DyLight 550 Labeled NBP1-54156R
DyLight 650 Labeled NBP1-54156C
FITC Labeled NBP1-54156F
HRP Labeled NBP1-54156H

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Earn rewards points by submitting your review for STK33 Antibody (NBP1-54156). Be the first to review this product and earn double rewards points!

Publications

Earn rewards if you have published using STK33 Antibody (NBP1-54156).

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
0.65 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol STK33
Entrez
Uniprot

Alternate Names for STK33 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine kinase 33
  • serine/threonine-protein kinase 33
Show More

Related Products by Gene

Background

STK33 (serine/threonine-protein kinase 33) belongs to Ca2+/calmodulin-dependent kinase family (CAMK) and mainly play role in the dynamic behavior of intermediate filament cytoskeleton by phosphorylation of vimentin. STK33 undergoes compulsory autophosphorylation and then phosphorylates vimentin in vitro specifically in the vimentin head domain. STK33 and vimentin have been shown to be interacting protein partners in vivo also and STK33 has been proposed to be involved in the dynamics of intermediate filament assembly/disassembly via vimentin phosphorylation. Downregulation of STK33 by RNAi or inhibition of its kinase activity by overexpression of a kinase dead (KD) K145M STK33 protein conferred a lethal phenotype to mutant KRAS cell lines. Suppression of STK33 expression by either method resulted in inhibition of p70S6K and RPS6 phosphorylation but had no effect on the phosphorylation of AKT, ERK1/2, or mTOR, suggesting that STK33 is downstream from these nodes, but upstream from p70S6K.

Reviews for STK33 Antibody (NBP1-54156) (0)

There are no reviews for STK33 Antibody (NBP1-54156).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used STK33 Antibody (NBP1-54156)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for STK33 Antibody (NBP1-54156) (0)

There are no publications for STK33 Antibody (NBP1-54156).
By submitting a publication earn 200 points for your submission points towards our Rewards Program.
Have you published using STK33 Antibody (NBP1-54156)? Earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

View specific protocols for STK33 Antibody (NBP1-54156): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for STK33 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

H00003845-M01
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Immunofluorescence of monoclonal antibody to KRAS on HeLa cell . [antibody concentration 10 ug/ml]Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in HeLa ( Cat # L013V1 ).

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF...MORE

     1 Review

3 Publications
NBP2-14871
Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Sample (50 ug of whole cell lysate) A: Mouse brain 15% SDS PAGE; antibody diluted at 1:1000.Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF

NB120-2860
Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - purified calmodulin using NB120-5494 (right)and NB120-2860 (left).Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Immunohistochemistry was performed on normal biopsies of deparaffinized Rat testis tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0)  buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Calmodulin or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor.  Tissues were counterstained with hematoxylin and prepped for mounting.

Mouse Monoclonal
Species Human, Rat, Bacteria...MORE
Applications WB, ELISA, ICC/IF...MORE

H00000808-T01
Lane 1: CALM3 transfected lysate (16.39 KDa) Lane 2: Non-transfected lysate.CALM3 transfected lysate.


Species Human
Applications WB

AF7280

Sheep Polyclonal
Species Human
Applications WB

H00000814-M01
Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - Western blot analysis of CAMK4 over-expressed 293 cell line. Lane 2 cotransfected with CAMK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00000814-M01. GAPDH (36.1 kDa) used as loading control.Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (1A3) [H00000814-M01] - Immunofluorescence of monoclonal antibody to CAMK4 ( Cat # H00000814-M01 ) on HeLa cell.Immunohistochemistry-Paraffin: CaMKIV Antibody (1A3) [H00000814-M01] - Immunoperoxidase of monoclonal antibody to CAMK4 ( Cat # H00000814-M01 ) on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml]

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF...MORE

NB100-56651
Western Blot: PLK1 Antibody [NB100-56651] - analysis of HCT-116 lysate using PLK1 antibody at 1:2000.Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - analysis of human placenta using PLK1 antibody at 1:100.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, IHC-P

NBP1-82539
Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG spImmunocytochemistry/Immunofluorescence: NEK8 Antibody [NBP1-82539] - Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoskeleton (microtubules).Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539] - Staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC...MORE

NBP1-32343
Western Blot: YANK2 Antibody [NBP1-32343] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.Immunohistochemistry-Paraffin: YANK2 Antibody [NBP1-32343] - Paraffin-embedded Gastric CA, using antibody at 1:100 dilution.Western Blot: YANK2 Antibody [NBP1-32343] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:5000.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00056133-T01
Lane 1: PCDHB2 transfected lysate (87.89 KDa) Lane 2: Non-transfected lysate.PCDHB2 transfected lysate.


Species Human
Applications WB

MAB2999

Rat Monoclonal
Species Human
Applications WB

NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Mouse
Applications WB, ELISA, ICC/IF...MORE

28 Publications
NB110-57231
Western Blot: MST4 Antibody (EP1864Y) [NB110-57231] HeLa cell lysate.Immunohistochemistry: MST4 Antibody (EP1864Y) [NB110-57231] - Immunohistochemical analysis of paraffin-embedded human placenta using anti-Mst4 (NB110-57231).

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, FLOW, ICC/IF...MORE

1 Publication
NB110-57027
Western Blot: vH RAS Antibody (Y132) [NB110-57027] A: MCF7 B: PC12 cell lysate using a 1:500 dilution.Immunocytochemistry/Immunofluorescence: vH RAS Antibody (Y132) [NB110-57027] - Immunocytochemical staining of MCF7 cells using anti-H-Ras (C-term) (catalog #110-57027).Flow Cytometry: vH RAS Antibody (Y132) [NB110-57027] - Overlay histogram showing HeLa cells stained with Anti-GTPase HRAS antibody [Y132]  (red line).

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, FLOW, ICC/IF...MORE

3 Publications
NBP1-42039
Western Blot: Myoglobin Antibody (EPR2581Y) [NBP1-42039] - Pig skeletal muscle whole tissue lysate.Immunohistochemistry-Paraffin: Myoglobin Antibody (EPR2581Y) [NBP1-42039] - Showing negative staining in Normal liver tissue.Western Blot: Myoglobin Antibody (EPR2581Y) [NBP1-42039] (A) Human muscle (B) Mouse muscle and © Rat muscle tissue lysate using a 1:5000 dilution.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P...MORE

NBP2-14295
Immunohistochemistry-Paraffin: ANKS1B Antibody [NBP2-14295] Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules .

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

H00091754-M01
Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - Western Blot analysis of NEK9 expression in NIH/3T3(Cat # L018V1 ).Immunocytochemistry/Immunofluorescence: NEK9 Antibody (1F6) [H00091754-M01] - Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell . [antibody concentration 10 ug/ml]Western Blot: NEK9 Antibody (1F6) [H00091754-M01] - Western Blot analysis of NEK9 expression in HeLa ( Cat # L013V1 ).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF...MORE

AF6028
Western Blot: TTK Antibody [AF6028] - Lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 0.5 ug/mL of Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (R&D catalog # AF6028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (R&D catalog # HAF016). A specific band was detected for TTK at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions.Immunohistochemistry-Paraffin: TTK Antibody [AF6028] - TTK was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human TTK Antigen Affinity-purified Polyclonal Antibody (R&D catalog # AF6028) at 15 ug/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; R&D catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells.

Sheep Polyclonal
Species Human
Applications WB, IHC, IHC-P

Bioinformatics Tool for STK33

Discover related pathways, diseases and genes to STK33. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Pathways for STK33

View related products by pathway.

Research Areas for STK33

Find related products by research area.

PTMs for STK33

Learn more about PTMs related to STK33.

Blogs on STK33

There are no specific blogs for STK33, but you can read our latest blog posts.