STK33 Antibody 0.025 ml

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Western Blot: STK33 Antibody [NBP1-54156] - Detection of Stk33 in HEK293 cell lysate.
Immunocytochemistry/Immunofluorescence: STK33 Antibody [NBP1-54156] - ICC staining of STK33 in HEK293 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: STK33 Antibody [NBP1-54156] - IHC staining of STK33 in mouse testis.
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Ordering Information: NBP1-54156

  • Catalog Number
    NBP1-54156
  • Displayed Format
    Unconjugated
  • Other Formats
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STK33 Antibody Summary

Species Human, Mouse
Tested Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
STK33
Purity
Immunogen affinity purified
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STK33 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human STK33 (within residues 1-150). [Swiss-Prot Q9BYT3]
Localization
Cytoplasm - perinuclear region.

Species Reactivity

Human and mouse.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This STK33 antibody is useful for ICC/IF, IHC and Western blot where a doublet is seen ~57 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-54156AF405
Alexa Fluor (R) 488 Labeled NBP1-54156AF488
Alexa Fluor (R) 647 Labeled NBP1-54156AF647
Alexa Fluor (R) 700 Labeled NBP1-54156AF700
Biotin Labeled NBP1-54156B
DyLight 405 Labeled NBP1-54156V
DyLight 488 Labeled NBP1-54156G
DyLight 550 Labeled NBP1-54156R
DyLight 650 Labeled NBP1-54156C
FITC Labeled NBP1-54156F
HRP Labeled NBP1-54156H

Contact Information

Product PDFs

Reviews

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Publications

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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.025 ml
Concentration
0.65 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol STK33
Entrez
Uniprot

Alternate Names for STK33 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine kinase 33
  • serine/threonine-protein kinase 33
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Related Products by Gene

Background

STK33 (serine/threonine-protein kinase 33) belongs to Ca2+/calmodulin-dependent kinase family (CAMK) and mainly play role in the dynamic behavior of intermediate filament cytoskeleton by phosphorylation of vimentin. STK33 undergoes compulsory autophosphorylation and then phosphorylates vimentin in vitro specifically in the vimentin head domain. STK33 and vimentin have been shown to be interacting protein partners in vivo also and STK33 has been proposed to be involved in the dynamics of intermediate filament assembly/disassembly via vimentin phosphorylation. Downregulation of STK33 by RNAi or inhibition of its kinase activity by overexpression of a kinase dead (KD) K145M STK33 protein conferred a lethal phenotype to mutant KRAS cell lines. Suppression of STK33 expression by either method resulted in inhibition of p70S6K and RPS6 phosphorylation but had no effect on the phosphorylation of AKT, ERK1/2, or mTOR, suggesting that STK33 is downstream from these nodes, but upstream from p70S6K.

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Publications for STK33 Antibody (NBP1-54156) (0)

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Product General Protocols

View specific protocols for STK33 Antibody (NBP1-54156): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for STK33 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Bioinformatics Tool for STK33

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