SSEA3 Antibody (MC-631) (NB100-1832)

SSEA3 Antibody (MC-631) (NB100-1832) (0.1 ml)

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	Catalog Number	NB100-1832 (Sample Size Available)
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anti-Stage-specific embryonic antigen 3 antibody, anti-Stage specific embryonic antigen 3 antibody

SSEA3 Antibody (MC-631) Summary:

Species:	Hu, Mu	Gene:
Applications:	WB, ICC, IHC-Fr, IHC-P, IP	Purity:	IgM purified
Clonality:	Monoclonal	Host:	Rat
Specificity:	Rat Monoclonal to SSEA3 (MC-631) - Embryonic Stem Cell Marker
Publications:	NB100-1832 has been mentioned in at least 5 Publications

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SSEA3 Antibody (MC-631) Details:

Clone:	MC-631
Isotype:	IgM
Immunogen:	4-8 cell stage mouse embryos.
Marker:	Embryonic Stem Cell Marker

Species Reactivity:

This antibody reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.

Applications:

Uses:	This antibody is useful for Immunohistochemistry on paraffin-embedded and frozen sections, Immnocytochemistry, Immunoprecipitation and Western blot.
Dilutions:	Immunocytochemistry 1:50 - 1:100, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	1.0 mg/ml
Conjugated Antibodies:	5 Conjugated Antibodies

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer:	Tris-glycine, 150mM NaCl
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Related Genes:

Background:

SSEA3 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

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Reviews (1)

Anonymous on 8/6/2010

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SSEA3 Antibody (MC-631) Images (3)

	Immunocytochemistry: SSEA3 Antibody (MC-631) [NB100-1832] - HeLa cells stained NB100-1832 (Green) detected with DyLight Fluor 488 conjugated anti-rat IgM secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

	Immunocytochemistry: SSEA3 Antibody (MC-631) - Embryonic Stem Cell Marker [NB100-1832] - Immunoctyochemical analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.

	Flow Cytometry: SSEA3 Antibody [NB100-1832] - FACS staining of NTERA-2 cells using NB100-1832 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rat IgM secondary antibody.

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Reviewed by:
Anonymous 8/6/2010

Application:	IHC-P
Sample Tested:	HeLa cells
Species:	Human
Sample Preparation:	Formalin-fixed, paraffin embedded
Sample Pretreatment:	None
Lot:	Lot A
Results:	Formalin-fixed, paraffin embedded HeLa cells display immunoreactivity to SSEA3

Blocking Buffer:	2% BSA
Blocking Time:	15 minutes
Blocking Temperature:	20

Dilution Ratio:	1:100
Incubation Time:	one hour
Incubation Temperature:	20 20
Incubation Dilution Buffer:	PBS

Description:	Biotinylated goat anti-mouse
Manufacturer Catalog #:	Vector
Dilution Ratio:	1:1500

Visualization Method:	DAB
Did you use controls?:	Yes
Positive Control:	HeLa with primary Ab
Negative Control:	HeLa with isotype negative control Ab
Additional Comments:	Vectastain ABC kit for 20 minutes prior to the DAB step.