SR-BI Antibody (NB400-104)
SR-BI Antibody
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anti-SCARB1 antibody, anti-CD36 AND LIMPII ANALOGOUS 1 antibody, member 1 antibody, anti-thrombospondin receptor-like 1 antibody, anti-Thrombospondin receptor like 1 antibody, anti-SR-BI antibody, anti-SRBI antibody, anti-SR-B1 antibody, anti-SRB1 antibody, anti-SR BI antibody, anti-Scavenger Receptor-BI antibody, anti-Scavenger Receptor-B1 antibody, anti-scavenger receptor class B type III antibody, anti-Scavenger Receptor Class B Type 1 antibody, anti-scavenger receptor class B, anti-Scavebger Receptor Class B Member 1 antibody, anti-SCARB1 antibody, anti-MGC138242 antibody, anti-HCLA 1 antibody, anti-Collagen type I receptor antibody, anti-Collagen type I receptor, anti-CLA1 antibody, anti-CLA 1 antibody, anti-CD36L1 antibody, anti-CD36 Antigen like 1 antibody, anti-CD36 antigen antibody, anti-CD36 and LIMPII analogous 1 antibody, |
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SR-BI Antibody Summary: | | Gene: | SCARB1 |
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| Purity: | Affinity purified |
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| Host: | Rabbit |
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| | Specificity: | NB400-104 is specific for the SR-BI. |
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| Publications: | Antibody has been mentioned in at least 60 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional SR-BI Products SR-BI Antibody Details: | Immunogen: | A C-terminal peptide containing residues from mouse Scavenger Receptor-BI (within residues 450-509). |
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Reacts with mouse, rat, and mink SR-BI and the long form of human CLA-I. |
| Uses: | Western blot, histochemistry, and immunoprecipitation. In western blot experiments with this SR-B1 a band of ~82 kDa is seen in tissues that express SR-BI such as liver, ovary and adrenals and to as lesser extent testes, heart and mammary gland. A band is often also seen at ~57 kDa representing the unglycosylated form of SR-BI. Also works for immunohistochemistry and immunoprecipitation. |
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| Dilutions: | Flow Cytometry, Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:1000 -1:5000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 3.0 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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| Conjugated Antibodies: | 1 Conjugated Antibodies |
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Notes: Licensed to Novus Biologicals LLC under U.S. Patent No 6,350,859. Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate [pH 7-8] buffer. |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
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Images (5) Reviews (1) Related Products (22) Exploring SR-BI Working with SR-BI
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with SR-BI
SR-BI Antibody Images (5) Immunohistochemistry: SR-BI antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal. |  Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - Detection of SR-BI (Green) in Hela cells using NB400-104 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258. |  Western Blot: SR-BI antibody [NB400-104] - Detection of SR-BI in mouse liver lysate (25 ug), using NB400-104 (lot J) at a 1:1,000 dilution, shows distinct band at 82 kDa. |  FACS analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago. |  Immunohistochemistry: SR-BI antibody [NB400-104] - Staining of adrenal, cortex using NB400-104. |
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Reviewed by:
B.M. Tao 11/23/2011
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Application:
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FACS
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Sample Tested:
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Mouse
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Species:
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Mouse
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Sample Pretreated:
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paraformaldehyde
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Results:
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The signal is strong and clear
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Dilution Ratio:
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100x
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Incubation Time:
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1 hour
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Incubation Temperature:
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room temperature room temperature
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Description:
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Goat anti-rabbit IgG FITC
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Visualization Method/Equipment:
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LSM 700 Confocal Microscopy
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Publications (60)SR-BI Antibody Product Specific:- Koller E, Vincent TM, Chappell A, et al. Mechanisms of single-stranded phosphorothioate modified antisense oligonucleotide accumulation in hepatocytes. Nucleic Acids Res. 2011 Feb 23.[PMID 21345934]
- Taketa Y, Yoshida M, Inoue K, et al. Differential stimulation pathways of progesterone secretion from newly formed corpora lutea in rats treated with ethylene glycol monomethyl ether, sulpiride, or atrazine. Toxicol. Sci. 2011 Jun.[PMID 21427058]
more... - Ji A, Meyer JM, Cai L, et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 2011 Apr 8.[PMID 21481393]
- Jenkitkasemwong S, Broderius M, Nam H, et al. Anemic copper-deficient rats, but not mice, display low hepcidin expression and high ferroportin levels. J Nutr. 2010 Apr;140(4):723-30. [PMID: 20164366](Mouse,Rat, WB)
- Haid S, Windisch MP, Bartenschlager R, et al. Mouse-specific residues of claudin-1 limit hepatitis C virus genotype 2a infection in a human hepatocyte cell line. J Virol. 2010 Jan;84(2):964-75. [PMID: 19889758]
- Mailhot G, Ravid Z, Barchi S, et al. CFTR knockdown stimulates lipid synthesis and transport in intestinal Caco-2/15 cells. Am J Physiol Gastrointest Liver Physiol. 2009 Dec;297(6):G1239-49. [PMID: 19808659]
- Arakawa R, Tsujita M, Iwamoto N, et al. Pharmacological inhibition of ABCA1 degradation increases HDL biogenesis and exhibits antiatherogenesis. J Lipid Res. 2009 Nov;50(11):2299-305. [PMID: 19458386]
- Haid S, Windisch MP, Bartenschlager R, et al. Mouse-specific Residues of Claudin-1 limit HCV Genotype 2a Infection in a Human Hepatocyte Cell Line. J Virol. 2009 Nov 4. [PMID: 19889758]
- Xu G, Watanabe T, Iso Y, et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res 2009;105(5):500-510. [pubmed: 19644050]
- Hongo S, Watanabe T, Arita S, et al. Leptin modulates ACAT1 expression cholesterol efflux from human macrophages. Am J Physiol Endocrinol Metab 2009;297(2):E474-482. [pubmed: 19625677]
- Bujold K, Rhainds D, Jossart C, et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res 2009;83(3):457-464. [pubmed: 19377069]
- Benedicto I, Molina-Jimenez F, Bartosch B, et al. The Tight Junction-Associated Protein Occludin Is Required for a Postbinding Step in Hepatitis C Virus Entry Infection. J Virol 2009;83(16):8012-8020. [pubmed: 19515778]
- Goti, D., et al. Effects of lipoprotein lipase on uptake and transcytosis of low density lipoprotein (LDL) and LDL-associated alpha-tocopherol in a porcine in vitro blood-brain barrier model. J Biol Chem. 2002 Aug 9;277(32):28537-44. Epub 2002 May 24.
- Witt, W., et al. Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells. J Lipid Res. 2000 Dec;41(12):2009-16.
- Xu G, Watanabe T, Iso Y, et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res 2009:CIRCRESAHA.109.193870. [pubmed: 19644050]
- Liu J, Huan C, Chakraborty M, et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res 2009;105(3):295-303. [pubmed: 19590047]
- Atshaves BP, McIntosh AL, Martin GG, et al. Overexpression of sterol carrier protein-2 differentially alters hepatic cholesterol accumulation in cholesterol-fed mice. J Lipid Res 2009;50(7):1429-1447. [pubmed: 19289417]
- Sane, A.T., et al. Localization and role of NPC1L1 in cholesterol absorption in human intestine. J Lipid Res. 2006 Oct;47(10):2112-20. Epub 2006 Jul 7.
- Azhar, S., et al. LDL and cAMP cooperate to regulate the functional expression of the LRP in rat ovarian granulosa cells. J Lipid Res. 2006 Nov;47(11):2538-50. Epub 2006 Aug 23.
- Zhong, J., et al. Persistent hepatitis C virus infection in vitro: coevolution of virus and host. J Virol. 2006 Nov;80(22):11082-93. Epub 2006 Sep 6.
- Zhang, C., et al. NO-1886 upregulates ATP binding cassette transporter A1 and inhibits diet-induced atherosclerosis in Chinese Bama minipigs. J Lipid Res. 2006 Sep;47(9):2055-63. Epub 2006 Jun 28.
- Yesilaltay, A., et al. Effects of hepatic expression of the high-density lipoprotein receptor SR-BI on lipoprotein metabolism and female fertility. Endocrinology. 2006 Apr;147(4):1577-88. Epub 2006 Jan 12.
- Wadsack, C., et al. Intrauterine growth restriction is associated with alterations in placental lipoprotein receptors and maternal lipoprotein composition. Am J Physiol Endocrinol Metab. 2007 Feb;292(2):E476-84. Epub 2006 Sep 26.
- Timmers, C., et al. E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop. Mol Cell Biol. 2007 Jan;27(1):65-78.
- Hullinger, Thomas G., et al. p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain. J Biol Chem. 2001 Dec 14;276(50):46807-14. Epub 2001 Oct 3.
- Burke KT, Colvin PL, Myatt L, et al. Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster. J Lipid Res 2009;50(6):1146-1155. [pubmed: 19122238]
- Bujold K, Rhainds D, Jossart C, et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res 2009:cvp118. [pubmed: 19377069]
- Kim HJ, Moradi H, Yuan J, et al. Renal mass reduction results in accumulation of lipids dysregulation of lipid regulatory proteins in the remnant kidney. Am J Physiol Renal Physiol 2009:90761.92008 [pubmed: 19357177]
- Karasinska JM, Rinninger F, Lutjohann D, et al. Specific Loss of Brain ABCA1 Increases Brain Cholesterol Uptake Influences Neuronal Structure Function. J Neurosci 2009;29(11):3579-3589 [pubmed: 19295162]
- Cherian-Shaw M, Puttabyatappa M, Greason E, et al. Expression of Scavenger Receptor-BI Low-Density Lipoprotein Receptor Differential Use of Lipoproteins to Support Early Steroidogenesis in Luteinizing Macaque Granulosa Cells. Endocrinology. 2009 Feb;150(2):957-65. [pubmed: 18832102]
- Pou J, Rebollo A, Roglans N, et al. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood). 2008 Dec;233(12):1572-82. Epub 2008 Oct 10.
- Shimizu E, Kawahara K, Kajizono M, et al. IL-4-induced selective clearance of oligomeric beta-amyloid peptide(1-42) by rat primary type 2 microglia. J Immunol. 2008 Nov 1;181(9):6503-13.
- Martin GG, Atshaves BP, McIntosh AL, et al. Liver fatty acid-binding protein gene-ablated female mice exhibit increased age-dependent obesity. J Nutr. 2008 Oct;138(10):1859-65.
- Iwao Y, Nakajou K, Nagai R, et al. CD36 is one of important receptors promoting renal tubular injury by advanced oxidation protein products. Am J Physiol Renal Physiol. 2008 Dec;295(6):F1871-80. Epub 2008 Oct 29.
- Bulat N, Waeber G, Widmann C. LDLs stimulate p38 MAPKs and wound healing through SR-BI independently of Ras and PI3 kinase. J Lipid Res. 2009 Jan;50(1):81-9. Epub 2008 Aug 28.
- Karpac J, Czyzewska K, Kern A, et al. Failure of adrenal corticosterone production in POMC-deficient mice results from lack of integrated effects of POMC peptides on multiple factors. Am J Physiol Endocrinol Metab. 2008 Aug;295(2):E446-55. Epub 2008 Jun 17.
- van der Velde AE, Vrins CLJ, van den Oever K, et al. Regulation of direct transintestinal cholesterol excretion in mice. Am J Physiol Gastrointest Liver Physiol. 2008 Jul;295(1):G203-G208. Epub 2008 May 29.
- Li L, Thompson PA, Kitchens RL. Infection induces a positive acute phase apolipoprotein E response from a negative acute phase gene: role of hepatic LDL receptors. J Lipid Res. 2008 Aug;49(8):1782-93. Epub 2008 May 22.
- Frank PG, Pavlides S, Cheung MWC, et al. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol. 2008 Jul;295(1):C242-8. Epub 2008 May 28.
- Tancevski I, Wehinger A, Demetz E, et al. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology. 2008 Jul;149(7):3708-12. Epub 2008 Apr 3.
- During A, Doraiswamy S, Harrison EH. Xanthophylls are preferentially taken up compared with beta-carotene by retinal cells via a SRBI-dependent mechanism. J Lipid Res. 2008 Aug;49(8):1715-24. Epub 2008 Apr 19.
- Montoudis, A., Seidman, E., Boudreau, F., et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res. 2008 May;49(5):961-72. Epub 2008 Jan 30.
- Drover VA, Nguyen DV, Bastie CC, et al. CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice. J Biol Chem. 2008 May 9;283(19):13108-15. Epub 2008 Mar 10.
- Valiyaveettil, M., N. Kar, et al. (2008). Oxidized high-density lipoprotein inhibits platelet activation and aggregation via scavenger receptor BI. Blood. 2008 Feb 15;111(4):1962-71. Epub 2007 Nov 9.
- Ashraf, M. Z., N. S. Kar, et al. Specific oxidized phospholipids inhibit scavenger receptor bi-mediated selective uptake of cholesteryl esters. J Biol Chem. 2008 Apr 18;283(16):10408-14. Epub 2008 Feb 19.
- Tietge UJF, Nijstad N, Havinga R, et al. Secretory phospholipase A2 increases SR-BI-mediated selective uptake from HDL but not biliary cholesterol secretion. J Lipid Res. 2008 Mar;49(3):563-71. Epub 2007 Nov 25.
- Barth H, Schnober EK, Neumann-Haefelin C, et al. Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells. J Virol. 2008 Apr;82(7):3466-79. Epub 2008 Jan 23.
- Yvan-Charvet L, Pagler TA, Wang N, et al. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J Lipid Res. 2008 Jan;49(1):107-14. Epub 2007 Oct 24.
- Eacker SM, Agrawal N, Qian K, et al. Hormonal regulation of testicular steroid and cholesterol homeostasis. Mol Endocrinol. 2008 Mar;22(3):623-35. Epub 2007 Nov 21.
- During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res. 2007 Oct;48(10):2283-94. Epub 2007 Jul 20.
- Atshaves BP, McIntosh AL, Payne HR, et al. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res. 2007 Oct;48(10):2193-211. Epub 2007 Jul 3.
- Qu, Shen., et al. Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice. J Lipid Res. 2007 Jul;48(7):1476-87. Epub 2007 Apr 16.
- Thomas-Moya, E., et al. The age-related paraoxonase 1 response is altered by long-term caloric restriction in male and female rats. J Lipid Res. 2006 Sep;47(9):2042-8. Epub 2006 Jun 30.
- Harder, C.J., et al. Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy. J Lipid Res. 2006 Mar;47(3):492-503. Epub 2005 Dec 7.
- Akpovi, C.D., et al. The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. J Lipid Res. 2006 Oct;47(10):2233-47. Epub 2006 Jul 21.
- Johansson, L., et al. Selective thyroid receptor modulation by GC-1 reduces serum lipids and stimulates steps of reverse cholesterol transport in euthyroid mice. Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10297-302. Epub 2005 Jul 8.
- Brodeur, M.R. et al. Physiological importance of SR-BI in the in vivo metabolism of human HDL and LDL in male and female mice. J Lipid Res. 2005 Apr;46(4):687-96. Epub 2005 Jan 16.
- Lalanne, F., et al. Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells. J Lipid Res. 2005 Jun;46(6):1312-9. Epub 2005 Mar 1.
- Lemieux, C., et al. Hypolipidemic action of the SERM acolbifene is associated with decreased liver MTP and increased SR-BI and LDL receptors. J Lipid Res. 2005 Jun;46(6):1285-94. Epub 2005 Mar 1.
- Han, J., et al. Functional interplay between the macrophage scavenger receptor class B type I and pitavastatin (NK-104). Circulation. 2004 Nov 30;110(22):3472-9. Epub 2004 Nov 22.
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Related ProductsConjugated AntibodiesPositive ControlsAntibody Packs (1)Lysates (1)Peptides and ProteinsPrimary Antibodies (16)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| SR-BI Antibody | NB400-101 | Mu | FACS, ICC, IF, IHC, IP, WB | (2) | (59) | | | | SR-BI Antibody | NB400-113 | Hu, Mu | B/N, ICC, IF, IP | (2) | (5) | | | | SR-BI Antibody | NB400-131 | Mu | ICC, IF, WB | (1) | (4) | | | | SR-BI Antibody (EP1556Y) | NB110-57591 | Hu, Mu, Rt | ICC, IHC-P, WB | (1) | (3) | | | | SR-BI Antibody | NBP1-77038 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ... and 11 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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