SR-BI Antibody (NB400-101)
SR-BI Antibody
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anti-SCARB1 antibody, anti-CD36 AND LIMPII ANALOGOUS 1 antibody, anti-thrombospondin receptor-like 1 antibody, anti-Thrombospondin receptor like 1 antibody, anti-SR-BI antibody, anti-SRBI antibody, anti-SR-B1 antibody, anti-SRB1 antibody, anti-SR BI antibody, anti-Scavenger Receptor-BI antibody, anti-Scavenger Receptor-B1 antibody, anti-scavenger receptor class B type III antibody, anti-Scavenger Receptor Class B Type 1 antibody, anti-scavenger receptor class B, anti-Scavebger Receptor Class B Member 1 antibody, anti-SCARB1 antibody, anti-MGC138242 antibody, anti-HCLA 1 antibody, anti-Collagen type I receptor antibody, anti-Collagen type I receptor, anti-CLA1 antibody, anti-CLA antibody, anti-CLA 1 antibody, anti-CD36L1 antibody, anti-CD36 Antigen like 1 antibody, anti-CD36 antigen antibody, anti-CD36 and LIMPII analogous 1 antibody, |
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SR-BI Antibody Summary: | | Gene: | SCARB1 |
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| Purity: | Affinity purified |
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| Host: | Rabbit |
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| | Specificity: | NB400-101 is specific for SR-BI. |
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| Publications: | Antibody has been mentioned in at least 59 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional SR-BI Products SR-BI Antibody Details: | Immunogen: | A C-terminal peptide containing residues from mouse Scavenger Receptor-BI (within residues 450-509). |
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| Localization: | Cell Membrane; multi-pass membrane protein |
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Reacts with mouse, rat and human SR-BI. |
| Uses: | In western blot experiments with NB400-101 a band of ~ 82 kDa is seen in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. This antibody has been used in immunoprecipitation and Flow Cytometry, as well. * NB 400-104 or NB 400-131 is recommended for immunohistochemical analysis of SR-B1. |
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| Dilutions: | Flow Cytometry, Immunocytochemistry 1:50 - 1:1000, Immunofluorescence 1:50 - 1:1000, Immunohistochemistry, Immunoprecipitation, Western Blot 1:1000-1:5000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 3.0 mg/ml |
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| Positive Controls: | 2 Positive Controls |
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Notes: Licensed to Novus Biologicals LLC under U.S. Patent No 6,350,859. Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | Tris-citrate/phosphate [pH 7-8] buffer. |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism.
In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
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Images (2) Reviews (0) Related Products (22) Exploring SR-BI Working with SR-BI
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with SR-BI
SR-BI Antibody Images (2) Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-101] - Detection of SR-BI (Green) in Hela cells using NB400-101 at a 1:200 dilution. Nuclei (Blue) were counterstained using Hoechst 33258. |  Western Blot: SR-BI antibody [NB400-101] - Detection of SR-BI in mouse liver lysate (20 ug) using NB 400-101. ECL detection 5 seconds. |
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Publications (59)SR-BI Antibody Product Specific:- Papale GA, Hanson PJ, Sahoo D. Extracellular disulfide bonds support scavenger receptor-BI-mediated cholesterol transport.Biochemistry. 2011 Jul 19;50(28):6245-54.[PMID 21675794]
- Wang DYC. Regulation of macrophage SR-BI by lipoproteins and inflammatory stimuli. 2011 Oct 1;
more... - Panzenboeck U., et al. ABCA1 and scavenger receptor class B, type I, are modulators of reverse sterol transport at an in vitro blood-brain barrier constituted of porcine brain capillary endothelial cells. J Biol Chem. 2002 Nov 8;277(45):42781-9. Epub 2002 Aug 28.
- Zhu P, Liu X, Treml LS, et al. Mechanism Regulatory Function of CpG Signaling via Scavenger Receptor B1 in Primary B Cells. J Biol Chem 2009;284(34):22878-22887. [pubmed: 19542230]
- Peng, Y., et al. Scavenger receptor BI (SR-BI) clustered on microvillar extensions suggests that this plasma membrane domain is a way station for cholesterol trafficking between cells and high-density lipoprotein. Mol Biol Cell. 2004 Jan;15(1):384-96. Epub 2003 Oct 3.
- Gaus, K., et al. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J. 2004 Mar;18(3):574-6. Epub 2004 Jan 20.
- Bland, M., et al. Differential requirement for steroidogenic factor-1 gene dosage in adrenal development versus endocrine function. Mol Endocrinol. 2004 Apr;18 (4):941-52. Epub 2004 Jan 15.
- Liang, K., et al. HMG-CoA reductase inhibition reverses LCAT and LDL receptor deficiencies and improves HDL in rats with chronic renal failure. Am J Physiol Renal Physiol. 2005 Mar;288(3):F539-44. Epub 2004 Oct 26.
- Witt, W., et al. Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells. J Lipid Res. 2000 Dec;41(12):2009-16.
- Parathath, S., et al. Glycine 420 near the C-terminal transmembrane domain of SR-BI is critical for proper delivery and metabolism of high density lipoprotein cholesteryl ester. J Biol Chem. 2004 Jun 11;279(24):24976-85. Epub 2004 Apr 1.
- Wu, J., et al. Hepatic ABCG5 and ABCG8 overexpression increases hepatobiliary sterol transport but does not alter aortic atherosclerosis in transgenic mice. J Biol Chem. 2004 May 28;279(22):22913-25. Epub 2004 Mar 25.
- Sahoo, D., et al. ABCA1- dependent lipid efflux to aplipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research.45:1122-1131, 2004. [PMID: 14993246] (WB, rat)
- Akiyama, T., et al. Peroxisome proliferator-activated receptor beta/delta regulates very low density lipoprotein production and catabolism in mice on a Western diet. J Biol Chem. 2004 May 14;279(20):20874-81. Epub 2004 Mar 4.
- Fu, T., et al. Overexpression of SR-BI by adenoviral vector reverses the fibrateinduced hypercholesterolemia of apolipoprotein E-deficient mice. J Biol Chem. 2003 Dec 26;278(52):52559-63. Epub 2003 Oct 21.
- Lundasen, T., et al. Leptin induces the hepatic high density lipoprotein receptor scavenger receptor B type I (SR-BI) but not cholesterol 7alpha-hydroxylase (Cyp7a1) in leptin-deficient (ob/ob) mice. J Biol Chem. 2003 Oct 31;278 (44):43224-8. Epub 2003 Aug 13.
- Kocher, O., et al. Targeted disruption of the PDZK1 gene in mice causes tissue-specific depletion of the high density lipoprotein receptor scavenger receptor class B type I and altered lipoprotein metabolism. J Biol Chem. 2003 Dec 26;278(52):52820-5. Epub 2003 Oct 8.
- Silver, D., et a. Identification of small PDZK1-associated protein, DD96/MAP17, as a regulator of PDZK1 and plasma high density lipoprotein levels. J Biol Chem. 2003 Aug 1;278(31):28528-32. Epub 2003 May 15.
- Chen, W., et al. Scavenger receptor-BI inhibits ATP-binding cassette transporter 1- mediated cholesterol efflux in macrophages. J Biol Chem. 2000 Oct 6;275(40):30794-800.
- Atshaves, B., et al. Sterol carrier protein-2 alters high density lipoprotein-mediated cholesterol efflux. J Biol Chem. 2000 Nov 24;275(47):36852-61.
- Green, H., et al. Evidence for a Trypanosoma brucei lipoprotein scavenger receptor. J Biol Chem. 2003 Jan 3;278(1):422-7. Epub 2002 Oct 24.
- Lambert, G., et al. The farnesoid X-receptor is an essential regulator of cholesterol homeostasis. J Biol Chem. 2003 Jan 24;278(4):2563-70. Epub 2002 Nov 5.
- Vishnyakova, T., et al. Binding and internalization of lipopolysaccharide by Cla-1, a human orthologue of rodent scavenger receptor B1. J Biol Chem. 2003 Jun 20;278(25):22771-80. Epub 2003 Mar 21.
- Sehayek, E., et al. Localization of the PE methylation pathway and SR-BI to the canalicular membrane: evidence for apical PC biosynthesis that may promote biliary excretion of phospholipid and cholesterol. J Lipid Res. 2003 Sep;44 (9):1605-13. Epub 2003 Jun 16.
- Burgess, J., et al. Phosphatidylinositol promotes cholesterol transport and excretion. J Lipid Res. 2003 Jul;44(7):1355-63. Epub 2003 Apr 16.
- Basso, F., et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholesterol and plasma HDL cholesterol concentrations. J Lipid Res. 2003 Feb;44(2):296-302. Epub 2002 Nov 4.
- Willner, E., et al. Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1262-7. Epub 2003 Jan 21.
- Husemann, J., et al. Scavenger receptor class B type I (SR-BI) mediates adhesion of neonatal murine microglia to fibrillar beta-amyloid. J Neuroimmunol. 2001 Mar 1;114(1-2):142-50.
- Frolov, A., et al. High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. A single- and multiphoton fluorescence approach. J Biol Chem. 2000 Apr 28;275(17):12769-80.
- Silver DL. A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver. J Biol Chem. 2002 Sep 13;277(37):34042-7. Epub 2002 Jul 15.
- Huang, Z., et al. ApoE-dependent sterol efflux from macrophages is modulated by scavenger receptor class B type I expression. J Lipid Res. 2002 Mar;43(3):375-82.
- Cai, S., et al. Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine. J Lipid Res. 2001 Jun;42(6):902-9.
- Tsuruoka, H., et al. Scavenger receptor class B type I is expressed in cultured keratinocytes and epidermis. Regulation in response to changes in cholesterol homeostasis and barrier requirements. J Biol Chem. 2002 Jan 25;277(4):2916-22. Epub 2001 Nov 13.
- Silver, David L. et al. High density lipoprotein (HDL) particle uptake mediated by scavenger receptor class B type 1 results in selective sorting of HDL cholesterol from protein and polarized cholesterol secretion. J Biol Chem. 2001 Jul 6;276 (27):25287-93. Epub 2001 Apr 11.
- Han, J., et al. Oxidized low density lipoprotein decreases macrophage expression of scavenger receptor B-I. J Biol Chem. 2001 May 11;276(19):16567-72. Epub 2001 Feb 6.
- Oghami, N., et al. Scavenger receptor class B type I-mediated reverse cholesterol transport is inhibited by advanced glycation end products. J Biol Chem. 2001 Apr 20;276(16):13348-55. Epub 2001 Jan 17.
- Nakaya H, Summers BD, Nicholson AC, et al. Atherosclerosis in LDLR-Knockout Mice Is Inhibited, but Not Reversed, by the PPAR{gamma} Lig Pioglitazone. Am J Pathol 2009;174(6):2007-2014. [pubmed: 19435790]
- Labonte ED, Camarota LM, Rojas JC, et al. Reduced absorption of saturated fatty acids and resistance to diet-induced obesity and diabetes by ezetimibe-treated and Npc1l1-/- mice. Am J Physiol Gastrointest Liver Physiol. 2008 Oct;295 (4):G776-83. Epub 2008 Aug 21.
- Ahras M., et al. Scavenger receptor class B type I localizes to a late endosomal compartment. J Lipid Res. 2008 Jul;49(7):1569-76. Epub 2008 Mar 29.
- Tietge UJ, et al. Secretory phospholipase A2 increases SR-BI-mediated selective uptake from HDL but not biliary cholesterol secretion. J Lipid Res. 2008 Mar;49(3):563-71. Epub 2007 Nov 25.
- Van Eck, M., et al. Scavenger receptor BI facilitates the metabolism of VLDL lipoproteins in vivo. J Lipid Res. 2008 Jan;49(1):136-46. Epub 2007 Oct 22.
- Zhang, Yi., et al. Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways. J Lipid Res. 2007 Feb;48(2):405-16. Epub 2006 Nov 1.
- Nakamura, T., et al. Regulation of SR-BI protein levels by phosphorylation of its associated protein, PDZK1. Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13404-9. Epub 2005 Sep 8.
- Johansson, L., et al. Selective thyroid receptor modulation by GC-1 reduces serum lipids stimulates steps of reverse cholesterol transport in euthyroid mice. PNAS. 102(29): 10297-10302, 2005. [PMID: 16006512] (Western blot, mouse)
- Lemieux, C., et al. The Hypolipidemic Action of the Selective Estrogen Receptor Modulator Acolbifene is Associated with Decreased Liver MTP Increased SR-B1 LDL Receptors. J. Lipid Res. 46:1285-1294, 2005. [PMID: 15741653] (Western blot, rat)
- Arai, T., et al. Decreased selective uptake of high density lipoprotein cholesteryl esters in apolipoprotein E knock-out mice. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):12050-5.
- Gaus, K., et al. Domain-specific lipid distribution in macrophage plasma embranes. J Lipid Res. 2005 Jul;46(7):1526-38. Epub 2005 May 1.
- Hsu, M. et al. Hepatitis C virus glycoprotiens mediate pH-dependent cell entry of pseudotyped retroviral particles.Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7271-6. [PMID: 12761383] (Flow cytometry, human)
- Han, J., et al. Functional interplay between the macrophage scavenger receptor class B type I and pitavastatin (NK-104). Circulation. 2004 Nov 30;110(22):3472-9. Epub 2004 Nov 22.
- Lehmann, K., et al. Dose-dependent alterations in gene expression and testosterone synthesis in the fetal testes of male rats exposed to di (n-butyl) phthalate. Toxicol Sci. 2004 Sep;81(1):60-8. Epub 2004 May 12.
- Levy, E., et al. Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine. J Cell Sci. 2004 Jan 15;117(Pt 2):327-37.
- Bocharov, A., et al. Targeting of scavenger receptor class B type I by synthetic amphipathic alpha-helical-containing peptides blocks lipopolysaccharide (LPS) uptake and LPS-induced pro-inflammatory cytokine responses in THP-1 monocyte cells. J Biol Chem. 2004 Aug 20;279(34):36072-82. Epub 2004 Jun 15.
- Tong, M.H., et al. Aberrant cholesterol transport and impaired steroidogenesis in Leydig cells lacking estrogen sulfotransferase. Endocrinology. 2004 May;145 (5):2487-97. Epub 2004 Jan 28.
- Thompsons, C., et al. Di(n-butyl) phthalate impairs cholesterol transport and steroidogenesis in the fetal rat testis through a rapid and reversible mechanism. Endocrinology. 2004 Mar;145(3):1227-37. Epub 2003 Nov 14.
- Vaziri ND, Liang KH. Acyl-coenzyme A:cholesterol acyltransferase inhibition ameliorates proteinuria, hyperlipidemia, lecithin-cholesterol acyltransferase, SRB-1, and low-denisty lipoprotein receptor deficiencies in nephrotic syndrome. Circulation. 2004 Jul 27;110(4):419-25. Epub 2004 Jul 19.
- Kashiwakura, Y., et al. Dynamin-2 regulates oxidized low-density lipoprotein-induced apoptosis of vascular smooth muscle cell. Circulation. 2004 Nov 23;110 (21):3329-34. Epub 2004 Nov 15.
- Merched AJ, Chan L. Absence of p21Waf1/Cip1/Sdi1 modulates macrophage differentiation and inflammatory response and protects against atherosclerosis. Circulation. 2004 Dec 21;110(25):3830-41. Epub 2004 Dec 13.
- Liang, C., et al. Increased CD36 protein as a response to defective insulin signaling in macrophages. J Clin Invest. 2004 Mar;113(5):764-73.
- Rhainds, D., et al. Localization and regulation of SR-BI in membrane rafts of HepG2 cells. J Cell Sci. 2004 Jul 1;117(Pt 15):3095-105.
- Wang, N. et al. Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1. J Biol Chem. 2000 Oct 20;275(42):33053-8.
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Related ProductsPositive ControlsAntibody Packs (1)Lysates (1)Peptides and ProteinsPrimary Antibodies (16)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| SR-BI Antibody | NB400-104 | Hu, Mu, Rt | FACS, ICC, IF, IHC-P, IP, WB | (5) | (60) | | | | SR-BI Antibody | NB400-113 | Hu, Mu | B/N, ICC, IF, IP | (2) | (5) | | | | SR-BI Antibody | NB400-131 | Mu | ICC, IF, WB | (1) | (4) | | | | SR-BI Antibody (EP1556Y) | NB110-57591 | Hu, Mu, Rt | ICC, IHC-P, WB | (1) | (3) | | | | SR-BI Antibody | NBP1-77038 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ... and 11 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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