SP1 Antibody (NB600-233)
SP1 Antibody
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Distributor Data...
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anti-SP1 antibody, anti-Transcription factor Sp1 antibody, anti-TFSP1 antibody, |
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SP1 Antibody Summary: | | Gene: | SP1 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | | | Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional SP1 Products SP1 Antibody Details: | Immunogen: | The epitope recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 785) using the numbering given in entry XP_028606.4 (GeneID 6667). |
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Human Sp1. Reactivity with Mouse is likely owing to sequence similarity at the site to which the immunogen epitope maps. |
| Uses: | This antibody may be used for Western blot and immunoprecipitation. Immunohisto/cytochemistry has not been tested using this antibody. Suggested working dilutions: * Western Blot - 1:2,500-1:10,000 Immunoprecipitation - 1-4 ug/ml of lysate** *The investigator should determine the optimal working dilution for a specific application. **NB 600-232 was superior in initial tests of IP efficacy. |
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| Dilutions: | Immunohistochemistry-Paraffin 1:100 - 1:500, Immunoprecipitation 1 to 4 ug/ml lysate, Western Blot 1:2 |
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| Unit Size: | 0.1 mg |
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| Concentration: | 1.0 mg/ml |
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Notes: IHC Caption A - Detection of Human Sp1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. IHC Caption B - Detection of Human Sp1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate buffer, pH 7 to 8 |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Sp1 is one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
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Images (3) Reviews (0) Related Products (37) Exploring SP1 Working with SP1
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Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPeptides and ProteinsPrimary Antibodies (35)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| SP1 Antibody (1A5) | H00006667-M02 | Mu, Rt | ELISA, IF, IHC-P, WB | (6) | | | | | SP1 Antibody (4C8) | H00006667-M03 | Hu | ELISA, IF, IHC-P, WB | (5) | | | | | SP1 Antibody (4H6) | H00006667-M01 | Hu | ELISA, IF, IHC-P, WB | (5) | | | | | SP1 Antibody | NBP1-61413 | Hu, Mu, Rt | ELISA, IF, IHC, WB | (3) | | | | | SP1 Antibody | NBP1-62007 | Hu, Mu, Rt | ELISA, IF, IHC, WB | (3) | | | | | ... and 30 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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![WB and IP: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used at 0.04 mcg/ml for WB.](http://images.novusbio.com/images/p_442827508d9f9.jpg "WB and IP: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used at 0.04 mcg/ml for WB.")
![Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - See Notes, IHC Caption A](http://images.novusbio.com/images/SP1 Antibody-Immunohistochemistry-Paraffin-NB600-233-img0001.jpg "Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - See Notes, IHC Caption A")
![Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - See Notes, IHC Caption B](http://images.novusbio.com/images/SP1 Antibody-Immunohistochemistry-Paraffin-NB600-233-img0002.jpg "Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - See Notes, IHC Caption B")
