Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residues 700 and 740 using the numbering given in entry XP_028606.4 (GeneID 6667).
Localization:
Nuclear
Species Reactivity:
Human Sp1. Reactivity with Mouse is likely owing to sequence similarity at the site to which the immunogen epitope maps.
Applications:
Uses:
This antibody may be used for Western blot and immunoprecipitation. Immunohisto/cytochemistry has not been tested using this antibody.
Dilutions:
immunoprecipitation 1-4 ug/ml of lysate,
Western Blot 1:10,000- 1:25,000,
000-1:25,000
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Sp1 is one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
Western Blot and Immunoprecipitation: SP1 Antibody [NB600-232] - Detection of Human SP1 on HeLa whole cell lysate using NB600-232. Subsequently, NB600-232 was used at 0.04 mcg/ml for WB.