SOX2 Antibody (NB110-37235) | Novus Biologicals

SOX2 Antibody (NB110-37235) (0.025 ml)

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	Catalog Number	NB110-37235
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anti-MGC2413 antibody, anti-transcription factor SOX2 antibody, anti-ANOP3 antibody, anti-transcription factor SOX-2 antibody, anti-MCOPS3 antibody, anti-SRY-related HMG-box gene 2 antibody, anti-SRY (sex determining region Y)-box 2 antibody

SOX2 Antibody Summary:

Species:	Hu, Mu, Ch, Sh, Xp, Ze	Gene:	SOX2
Applications:	WB, FACS, ICC, IF, IHC-P	Purity:	Immunogen affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	SOX2 - Embryonic Stem Cell Marker
Publications:	NB110-37235 has been mentioned in at least 2 Publications

View Additional SOX2 Products

Lysates	Peptides and Proteins
Primary Antibodies	RNAi
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SOX2 Antibody Details:

Immunogen:	A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]
Localization:	Nuclear
Marker:	Embryonic Stem Cell Marker

Species Reactivity:

Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.

Applications:

Uses:	Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical, immunocytochemical/immunofluorescence, and Flow cytometry/analysis applications.
Dilutions:	Flow Cytometry 1:100, Immunocytochemistry 1:125-1:250, Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:125-1:250, Western Blot -0.5 ug/ml
Unit Size:	0.025 ml (also available: 0.1 ml)
Concentration:	1.46 mg/ml
Positive Controls:	4 Positive Controls

Packaging:

Storage:	Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Buffer:	Tris-glycine, 150mM NaCl
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: SOX2

Entrez:	6657 (Human)
Entrez:	20674 (Mouse)
Uniprot:	P48431 (Human)

Related Genes:

Background:

The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate.

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Reviews (1)

Wen- Wei Chang on 12/27/2011

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SOX2 Antibody Images (4)

	Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).

	Immunohistochemistry: SOX2 Antibody [NB110-37235] - Staining of uterus, endometrial glands using NB110-37235.

	Immunocytochemistry: SOX2 Antibody [NB110-37235] - ICC staining of NTera2 cells using NB110-37235 for SOX2 (Green) at a 1:50 dilution and NB100-690 for alpha-Tubulin (Red) at a 1:1000 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

	Flow Cytometry: SOX2 Antibody [NB110-37235] - FACS staining of NTERA-2 cells using NB110-37235 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

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Reviewed by:
Wen- Wei Chang 12/27/2011

Application:	Western Blot
Sample Tested:	Oral cancer cell line
Species:	Human
Results:	Good antibody for testing in Western Blot. Clear signal at 40 kDa.