SOX2 Antibody (NB110-37235)
SOX2 Antibody
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Distributor Data...
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anti-ysb antibody, anti-SRY-box 2 antibody, anti-SRY related HMG box gene 2 antibody, anti-RGD1565646 antibody, anti-MGC148683 antibody, anti-lcc antibody, anti-Delta EF2a antibody, anti-cb236 antibody, anti-Transcription factor SOX 2 antibody, anti-SOX 2 antibody, anti-Sex determining region Y box 2 antibody, anti-Transcription factor SOX-2 antibody, anti-SRY related HMG box 2 antibody, anti-SRY box containing gene 2 antibody, anti-SRY (sex determining region Y) box 2 antibody, anti-Sex determining region Y-box 2 antibody, anti-MGC2413 antibody, anti-MCOPS3 antibody, anti-ANOP3 antibody |
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SOX2 Antibody Summary: | | Gene: | SOX2 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | SOX2 - Embryonic Stem Cell Marker |
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| Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional SOX2 Products SOX2 Antibody Details: | Immunogen: | A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431] |
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| Localization: | Nuclear |
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| Marker: | Embryonic Stem Cell Marker |
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Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins. |
| Uses: | Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical, immunocytochemical/immunofluorescence, and Flow cytometry/analysis applications. |
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| Dilutions: | Flow Cytometry 1:100, Immunocytochemistry 1:125-1:250, Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:125-1:250, Western Blot -0.5 ug/ml |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.81 mg/ml |
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| Positive Controls: | 4 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | Tris-glycine, 150mM NaCl |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate.
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Images (4) Reviews (1) Related Products (29) Exploring SOX2 Working with SOX2
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with SOX2
SOX2 Antibody Images (4) Immunocytochemistry: SOX2 Antibody [NB110-37235] - ICC staining of NTera2 cells using NB110-37235 for SOX2 (Green) at a 1:50 dilution and NB100-690 for alpha-Tubulin (Red) at a 1:1000 dilution. Nuclei (Blue) were counterstained using Hoechst 33258. |  Flow Cytometry: SOX2 Antibody [NB110-37235] - FACS staining of NTERA-2 cells using NB110-37235 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody. |  Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml). |  Immunohistochemistry: SOX2 Antibody [NB110-37235] - Staining of uterus, endometrial glands using NB110-37235. |
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Reviewed by:
Wen- Wei Chang 12/27/2011
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Application:
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Western Blot
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Sample Tested:
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Oral cancer cell line
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Species:
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Human
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Results:
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Good antibody for testing in Western Blot. Clear signal at 40 kDa.
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Blocking Buffer:
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5% Skim Milk
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Description:
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HRP-conjugated anti-rabbit IgG (Perkin Elmer)
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Method:
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ECL Plus
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Did you observe bands?:
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Yes
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Specific Bands:
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40 kDa
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Publications (1)
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsLysates (1)Peptides and ProteinsPrimary Antibodies (18)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| SOX2 Antibody (EPR3131) | NBP1-40712 | Hu, Mu | ICC, IHC-P, WB | (4) | | | | | SOX2 Antibody | NB110-79875 | Hu, Mu, Rt | ELISA, IHC, WB | (1) | (3) | | | | SOX2 Antibody (EPR3130) | NBP1-40720 | Hu | IHC-P, IP, WB | (4) | | | | | SOX2 Antibody (10F10) | NBP1-51688 | Hu | ELISA, IF, IHC-P, WB | (3) | | | | | SOX2 Antibody | 29930002 | Hu | ELISA | (4) | | | | | ... and 13 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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