Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residue 1 and 50 of human Src-associated in mitosis 68 kDa protein (KH domain containing, RNA binding, signal transduction associated 1) using the numbering given in entry NP_006550.1 (GeneID 10657).
Species Reactivity:
Human
Applications:
Uses:
This antibody is useful for western blot and immunoprecipitation.
Dilutions:
Immunohistochemistry-Frozen 1:100 - 1:500, Immunoprecipitation 2-5 mcg/mg lysate, Western Blot 1:2000-1:10000
Unit Size:
0.1 ml
Concentration:
0.2 mg/ml
Notes:
IHC Caption A - Detection of Human SAM68 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SAM68 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
IHC Caption B - Detection of Human SAM68 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SAM68 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
IHC Caption C - Detection of Mouse SAM68 by Immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-SAM68 used at a dilution of 1:100. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-buffered Saline containing 0.1% BSA
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Western Blot and Immunoprecipitation: SAM68 Antibody [NBP1-19151]- Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153.
Immunohistochemistry: SAM68 Antibody [NBP1-19151]-Detection of Human Rictor. FFPE section of human prostate carcinoma. Affinity purified rabbit anti-Rictor used at a dilution of 1:250.
Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - See Notes, IHC Caption A
Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - See Notes, IHC Caption B
Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - See Notes, IHC Caption C