Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials Summary:
| |
|
| Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Raw 264.7 Whole Cell - T0901317 treated Products
Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials Details:
Lysate Details:
| Type: | Cell | Life Stage: | Embryonic |
| Tissue: | Blood Lymph | Protein State: | Denatured |
| Tissue Condition: | Tumor | Sub-cellular Fraction: | Whole |
| Uses: | For western blot 10ul should be loaded per lane. |
|---|
| Dilutions: | Western Blot 0.01ml per lane |
|---|
| Unit Size: | 2 vials |
|---|
| Concentration: | Concentration is not relevant for this product. Please see the protocols for proper use of this product. |
|---|
|
Notes:
Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 10% prior to use. NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Samples should be heated to 65C for 15 minutes prior to loading.
Packaging:
| Storage: | Store at -80 °C. Avoid freeze-thaw cycles. |
|---|
| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
|---|
Background:
T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.
![Western Blot: ABCA1 Antibody [NB400-105] using RAW 264.7 Whole cell Lysate Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).](http://images.novusbio.com/images/108726_1.jpg "Western Blot: ABCA1 Antibody [NB400-105] using RAW 264.7 Whole cell Lysate Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).")