Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials (NBP1-30159)

Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials (NBP1-30159) ( 2 vials)

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	Catalog Number	NBP1-30159
	Size(s)	2 vials	Price:
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Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials Summary:

Species:		Gene:
Applications:	WB	Purity:
Clonality:		Host:
Publications:

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Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials Details:

Localization:	Macrophage
Marker:	T0901317 treated
Kit Components

Lysate Details:

Type:	Cell	Life Stage:	Embryonic
Tissue:	Blood Lymph	Protein State:	Denatured
Tissue Condition:	Tumor	Sub-cellular Fraction:	Whole

Applications:

Uses:	For western blot 10ul should be loaded per lane.
Dilutions:	Western Blot 0.01ml per lane
Unit Size:	2 vials
Concentration:	Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Notes:

Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 10% prior to use. NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Samples should be heated to 65C for 15 minutes prior to loading.

Packaging:

Storage:	Store at -80°C. Avoid freeze-thaw cycles.
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Related Genes:

Background:

T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.

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Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials Images (1)

	Western Blot: ABCA1 Antibody [NB400-105] using RAW 264.7 Whole cell Lysate Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).