Jump to Information on Raw 264.7 Whole Cell - T0901317 treated Kit (NBP1-30159)
Raw 264.7 Whole Cell - T0901317 treated Other Kit Summary
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Tested Applications
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WB |
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Kit Type
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Other Kit |
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Guarantee Plus
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All of our products are backed by our 100% guarantee to work for stated and predicted species and applications. |
Raw 264.7 Whole Cell - T0901317 treated Other Kit Details
| Marker |
T0901317 treated |
| Localization |
Macrophage |
Kit Components
Lysate Details for Raw 264.7 Whole Cell - T0901317 treated
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Type
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Cell |
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Life Stage
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Embryonic |
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Tissue
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Blood Lymph |
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Protein State
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Denatured |
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Tissue Condition
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Tumor |
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Subcellular Fraction
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Whole |
Applications/Dilutions
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Dilutions
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- Western Blot 0.01ml per lane
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Application Notes
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For western blot 10ul should be loaded per lane. |
Product PDFs
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Controls & Support Products
Notes
Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 10% prior to use. NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Samples should be heated to 65C for 15 minutes prior to loading.
Packaging, Storage & Formulations
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Storage
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Store at -80C. Avoid freeze-thaw cycles. |
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Unit Size
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2 vials |
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Concentration
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2.5 mg/ml |
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Limitations
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This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt. |
Background
T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.
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Product General Protocols
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Video Protocols
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![Western Blot: Raw 264.7 Whole Cell - T0901317 treated Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).](http://images.novusbio.com/fullsize2/Raw 264.7 Whole Cell - T0901317 treated Kit-Western Blot-NBP1-30159-img0003.jpg)
