Raji Whole Cell Lysate (NB800-PC11)

Raji Whole Cell Lysate (NB800-PC11) (0.5 mg)

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	Catalog Number	NB800-PC11
	Size(s)	0.5 mg	Price:
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Raji Whole Cell Lysate Summary:

Species:		Gene:
Applications:	WB	Purity:
Clonality:		Host:
Specificity:	Raji whole cell extract lysate
Publications:

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Lysates
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Lysate Details:

Type:	Cell	Life Stage:	Adult
Tissue:	Blood Lymph	Tissue Condition:	Tumor
Sub-cellular Fraction:	Whole

Applications:

Uses:	Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 0.015-0.03 ml depending on the size format of your gel.
Dilutions:	Western Blot
Unit Size:	0.5 mg
Concentration:	1 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at -80°C. Avoid freeze-thaw cycles.
Buffer:	1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Related Genes:

Background:

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

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Raji Whole Cell Lysate Images (1)

	Western Blot: Raji Whole Cell Lysate [NB800-PC11] - Coommassie stain of 0.02 ml of NB 800-PC11 separated on a 4-20% gradient gel under reducing conditions (lane 2). MW standard is shown in lane 1.

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Protocols

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Jump to: Positive Controls, Lysates

Related Products

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Other Positive Controls Note that Raji lysate is only applicable as a positive control for specific antibodies.

Lysates (4)

Name	Catalog#	Reactivity	Applications
Raji Whole Cell Lysate	NB820-59466	Hu	IP, WB
Raji Whole Cell Lysate	NBP2-10280	Hu	WB
Raji Whole Cell Lysate	NBL1-26471	Hu