Home » IgG (H+L) » IgG (H+L) Antibodies » Goat anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase]

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase]

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 (1 Review)
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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB730-AP
    • Availability
      Product Discontinued

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] Summary

Immunogen
Rabbit IgG. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos).
Localization
Secreted
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against nonimmunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:1000 - 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:1000 - 1:10000
Application Notes
ELISA, immunohisto/cytochemistry, and Western blotting.
Reviewed Applications
Read 1 Review rated 4
using
NB730-AP in the following applications:
Publications
Read Publications using
NB730-AP in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB730-AP)(3)

We have publications tested in 1 confirmed species: Rabbit.

We have publications tested in 3 applications: ELISA, ICC/IF, WB.


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ELISA
(1)
ICC/IF
(1)
WB
(1)
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Rabbit
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Review for IgG Antibody (NB730-AP) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB730-AP:
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WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Other 03/02/2009
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Summary

ApplicationWestern Blot
Sample Testedrecombinant protein, Sample Amount: 0.5 ug
SpeciesOther
LotP14

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Antibody (NB730-AP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

Array NB730-AP

Research Areas for IgG Antibody (NB730-AP)

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Blogs on IgG (H+L)

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Contact Information

Product PDFs

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Calculators

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
 
 Verified Customer
03/02/2009
Application: WB
Species: Other
read this review