Goat anti-Rabbit IgG (H+L) Secondary Antibody [HiLyte Fluor 750] Summary
| Immunogen |
Rabbit IgG |
| Specificity |
Rabbit IgG (H+L) |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Goat |
| Purity |
Immunogen affinity purified |
Applications/Dilutions
| Dilutions |
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence
|
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
0.01 mM phosphate, 150 mM NaCl, pH 7.2, with bovine serum albumin. |
| Preservative |
2mM Sodium Azide |
| Concentration |
1 mg/ml |
| Purity |
Immunogen affinity purified |
Background
HiLytePlus(TM) 750 is a new fluorescent labeling dye with the structure designed for maximum brightness when conjugated to macromolecules. Spectrally similar to Cy7 dye, HiLytePlus(TM) 750 is the longest-wavelength HiLytePlus(TM) dye currently available. The fluorescence signal of HiLytePlus(TM) 750 can be observed at 779 nm with the excitation at 751 nm. HiLytePlus(TM) 750-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. The goat anti-rabbit IgG is highly adsorbed with minimal cross-reaction to human, mouse, and rat serum protein. This secondary antibody is ideal for multi-fluorescence staining in which several primary antibodies from different species are involved.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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