RNA polymerase II Antibody (4H8) (NB200-598)

RNA polymerase II Antibody (4H8) (NB200-598) (0.1 ml)

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	Catalog Number	NB200-598 (Sample Size Available)
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RNA polymerase II Antibody (4H8) Summary:

Species:	Hu, Mu, Ye	Gene:	POLR2A
Applications:	WB, ChIP, ELISA, ICC, IF, IP	Purity:	IgG purified
Clonality:	Monoclonal	Host:	Mouse
Specificity:	This recognises the C-terminal repeat of the largest subunit of RNA polymerase II, it recognises both unphosphorylated and phosphorylated forms.
Publications:	NB200-598 has been mentioned in at least 2 Publications

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Lysates	Peptides and Proteins
Primary Antibodies	RNAi
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RNA polymerase II Antibody (4H8) Details:

Clone:	4H8
Isotype:	IgG1
Immunogen:	10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5 YSPTSpPS (Human).

Species Reactivity:

Cross-reacts with Human, S. cerevisiae (yeast) and mouse. Expected to cross-react with Drosophilia melanogaster, Hamster, S. pombe, Arabidopsis thaliana due to sequence homology - all of these species contain at least one or more 100% identical repeats. Not yet tested in other species

Applications:

Uses:	This RNA polymerase II Antibody (4H8) is useful for ChIP, ICC/IF, ELISA, Immunoprecipitation and Western blot, where a band can be seen at approximately 217 kDa. Optimal dilutions/concentrations should be determined by the end user.
Dilutions:	Chromatin Immunoprecipitation, ELISA, Immunocytochemistry 1:100 - 1:1000, Immunofluorescence 1:100 - 1:1000, Immunoprecipitation, Western Blot 1:1000
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	PBS
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: POLR2A

Entrez:	5430 (Human)

Related Genes:

Background:

RNA Polymerase II is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.

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RNA polymerase II Antibody (4H8) (NB200-598) (0.1 ml)

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Publications (2)

RNA polymerase II Antibody (4H8) Product Specific:

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Positive Controls

Lysates (1)

Peptides and Proteins

Primary Antibodies (14)

RNAi (1)

Secondary Antibodies

RNA polymerase II in Context

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