Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residues 1 and 50 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984).
Species Reactivity:
Human. Reactivity with Mouse RFC4 is unlikely owing to divergence of sequence from the peptide used for immunization.
Applications:
Uses:
This antibody can be used for Western Blot. *The investigator should determine the optimal dilution for a specific application.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5. RFC4 is also known as replication factor C 37 kDa subunit, RF-C 37 kDa subunit, a1 37 kDa subunit, A1, RFC37, and MGC27291.
Western Blot: RFC4 Antibody [NB100-234] - Whole cell extract (50mcg) from MCF7 and 293T or nuclear extract (50 mcg) from HeLa cells. Affinity purified goat anti-RFC4 used at 0.5 mcg/ml (A) and 1 mcg/ml (B). Chemiluminescence detection
Collis, S., et al. Evasion of Early Cellular Response Mechanisms following Low Level Radiation-induced DNA Damage. J. Biol. Chem. 279: 49624-49632, 2004.