RFC4 Antibody (NB100-234)

RFC4 Antibody

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RFC4 Antibody Summary:
Species:Hu
Applications:WB
Clonality:Polyclonal
Gene:RFC4
Purity:Immunogen affinity purified
Host:Goat
 
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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RFC4 Antibody Details:
Immunogen:The epitope recognized by this antibody maps to a region between residues 1 and 50 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984).
Species Reactivity:

Human. Reactivity with Mouse RFC4 is unlikely owing to divergence of sequence from the peptide used for immunization.

Applications:
Uses:This antibody can be used for Western Blot. *The investigator should determine the optimal dilution for a specific application.
Dilutions:Western Blot 1:1000-1:10000
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: RFC4
Entrez5984 (Human)
Swiss ProtNP_002907.1 (Human)
 
Background:

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5. RFC4 is also known as replication factor C 37 kDa subunit, RF-C 37 kDa subunit, a1 37 kDa subunit, A1, RFC37, and MGC27291.

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Working with RFC4

RFC4 Antibody Images (1)
Western Blot: RFC4 Antibody [NB100-234] - Whole cell extract (50mcg) from MCF7 and 293T or nuclear extract (50 mcg) from HeLa cells. Affinity purified goat anti-RFC4 used at 0.5 mcg/ml (A) and 1 mcg/ml (B). Chemiluminescence detection
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Publications (1)

RFC4 Antibody Product Specific:

  1. Collis, S., et al. Evasion of Early Cellular Response Mechanisms following Low Level Radiation-induced DNA Damage. J. Biol. Chem. 279: 49624-49632, 2004.

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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RNAi (3)
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RFC4 in Context


Research Areas related to RFC4 (2):
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