RFC4 Antibody (NB100-233)

RFC4 Antibody

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RFC4 Antibody Summary:
Species:Hu
Applications:WB
Clonality:Polyclonal
Gene:RFC4
Purity:Immunogen affinity purified
Host:Goat
 
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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RFC4 Antibody Details:
Immunogen:The epitope recognized by this antibody maps to a region between residues 300 and 350 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984).
Species Reactivity:

Human. Probability of reactivity with Mouse RFC4 is good (22/23 aa are identical and 1/23aa is a conserved substitution with the peptide used for immunization.

Applications:
Uses:This antibody can be used for Western Blot. Suggested working dilutions: * Immunoprecipitation ND Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application.
Dilutions:Western Blot 1:500-1:5,000
Unit Size:0.1 mg
Concentration:Please see the vial label for concentration.
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: RFC4
Entrez5984 (Human)
Swiss ProtNP_002907.1 (Human)
 
Background:

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5. RFC4 is also known as replication factor C 37 kDa subunit, RF-C 37 kDa subunit, a1 37 kDa subunit, A1, RFC37, and MGC27291.

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Working with RFC4

RFC4 Antibody Images (1)
Western Blot: RFC4 Antibody [NB100-233] -
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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Positive Controls
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
RFC4 LysateNBL1-15294WB(1)

Antibody Pairs (1)
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RFC4 Antibody PairH00005984-PW1HuIP, WB

Lysates (3)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
RFC4 LysateH00005984-T01HuWB(2)

RFC4 LysateNBL1-15294WB(1)

RFC4 LysateH00005984-T02WB

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
RFC4 Recombinant ProteinH00005984-P01HuELISA, WB(1)

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Primary Antibodies (12)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
RFC4 Antibody (1C12)H00005984-M01HuELISA, IHC-P, RNAi, WB(5)

RFC4 AntibodyNBP1-33083HuIF, IHC-P, WB(3)

RFC4 AntibodyNB100-234HuWB(1)(1)

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RNAi (3)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
RFC4 RNAiH00005984-R01VHu(2)(4)

RFC4 RNAiH00005984-R04Hu(1)(4)

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Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Goat IgG Antibody [FITC]NB710-FGtICC, IHC-P

Goat IgG Antibody [Biotin]NB710-BGtELISA, ICC, IHC-P, WB

Goat IgG Antibody [Alkaline Phosphatase]NB710-APGtELISA, ICC, IHC-P, WB

Goat IgG Antibody [HRP]NB710-HGtELISA, ICC, IHC-P, WB(4)

Goat IgG Antibody [Cy3]NB120-6949GtELISA, FACS, ICC, IF, IHC-Fr, IHC-P

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RFC4 in Context


Research Areas related to RFC4 (2):
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