RFC4 Antibody (H00005984-M01)

RFC4 Antibody (1C12)

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RFC4 Antibody (1C12) Summary:
Species:Hu
Applications:ELISA, IHC-P, RNAi, WB
Clonality:Monoclonal
Gene:RFC4
Purity:IgG purified
Host:Mouse
Specificity:RFC4 - replication factor C (activator 1) 4, 37kDa
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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RFC4 Antibody (1C12) Details:
Clone:1C12
Isotype:IgG Mix Kappa
Immunogen:RFC4 (AAH17452, 254 a.a. ~ 363 a.a) partial recombinant protein with GST tag.
Epitope:GKEITEKVITDIAGVIPAEKIDGVFAACQS GSFDKLEAVVKDLIDEGHAATQLVNQLHDV VVENNLSDKQKSIITEKLAEVDKCLADGAD EHLQLISLCATVMQQLSQNC
Species Reactivity:

Human. Other species not tested.

Applications:
Uses:Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.
Dilutions:ELISA, Immunohistochemistry-Paraffin, Western Blot 1:500, RNAi Validation
Unit Size:0.1 mg
Concentration:Please see the vial label for concentration.
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:In 1x PBS, pH 7.2
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: RFC4
Entrez5984 (Human)
OMIM102577 (Human)
Swiss ProtAAH17452 (Human)
 
Background:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

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Working with RFC4

RFC4 Antibody (1C12) Images (5)
Western blot analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi or non-transfected control. Blot probed with H00005984-M01. GAPDH (36.1 kDa) used as loading control.Western Blot analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.

Lane 1: RFC4 transfected lysate(39.7 KDa).
Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to RFC4 ( Cat # H00005984-M01 ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]RFC4 monoclonal antibody (M01), clone 1C12 Western Blot analysis of RFC4 expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RFC4 in Context


Research Areas related to RFC4 (2):
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