RFC2 Antibody (H00005982-B02P)

RFC2 Antibody

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RFC2 Antibody Summary:
Species:Hu
Applications:ELISA, IF, IHC-P, WB
Clonality:Polyclonal
Gene:RFC2
Purity:Protein A purified
Host:Mouse
Specificity:RFC2 - replication factor C (activator 1) 2, 40kDa,
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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RFC2 Antibody Details:
Immunogen:RFC2 (NP_002905.2, 1 a.a. ~ 320 a.a) full-length human protein.
Epitope:MEVEAVCGGAGEVEAQDSDPAPAFSKAPGS AGHYELPWVEKYRPVKLNEIVGNEDTVSRL EVFAREGNVPNIIIAGPPGTGKTTSILCLA RALLGPALKDAMLELNASNDSMTDGAQQAL RRTMEIYSKTTRFALACNASDKIIEPIQSR CAVLRYTKLTDAQILTRLMNVIEKERVPYT DDGLEAIIFTAQGDMRQALNNLQSTFSGFG FINSENVFKVCDEPHPLLVKEMIQHCVNAN IDEAYKILAHLWHLG
Species Reactivity:

Human.

Applications:
Uses:Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot and also on transfected lysate in Western Blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence and immunohistochemistry (paraf
Dilutions:ELISA, immunofluorescence, Immunohistochemistry-Paraffin, Western Blot 1:500
Unit Size:0.05 mg
Concentration:Please see the vial label for concentration.
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:In 1x PBS, pH 7.2
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: RFC2
Entrez5982 (Human)
Swiss ProtNP_002905.2 (Human)
 
Background:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

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Working with RFC2

RFC2 Antibody Images (5)
Western Blot analysis of RFC2 expression in transfected 293T cell line by RFC2 MaxPab polyclonal antibody.

Lane 1: RFC2 transfected lysate(35.2 KDa).
Lane 2: Non-transfected lysate.
Immunoperoxidase of purified MaxPab antibody to RFC2 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RFC2 on HeLa cell. [antibody concentration 10 ug/ml]RFC2 MaxPab polyclonal antibody. Western Blot analysis of RFC2 expression in HL-60.
RFC2 MaxPab polyclonal antibody. Western Blot analysis of RFC2 expression in HeLa.
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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RFC2 in Context


Research Areas related to RFC2 (2):
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