Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981).
Species Reactivity:
Human. Probability of reacting with MouseRFC1 is good (25/33 aa are identical, 6/33 aa are conserved substitutions and 2/33 are non-conserved substitutions with the peptide used for immunization).
Applications:
Uses:
This antibody can be used for Western Blot and immunoprecipitation. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 Immunoprecipitation 2-6 ug/mg extract Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application.
Dilutions:
immunoprecipitation 2 to 6 ug/mg,
Western Blot 1:1,00 to 1:10,000
Unit Size:
0.1 mg
Concentration:
Please see the vial label for concentration.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]