Peripherin Antibody (NB300-137)
Peripherin Antibody
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Distributor Data...
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anti-NEF4 antibody, anti-Peripherin Variant 61 antibody, anti-neurofilament 4 antibody, |
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Peripherin Antibody Summary: | | Gene: | PRPH |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | Specifically recognizes peripherin, showing no cross reactivity with other Class III intermediate filament proteins. |
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| Publications: | Antibody has been mentioned in at least 3 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Peripherin Products Peripherin Antibody Details: | Immunogen: | Recombinant full length rat peripherin |
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| Localization: | Neuronal Marker |
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| Marker: | Nociceptive Neuron Marker |
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Human and all other mammalian species to date. Results on avian tissues are however not conclusive. |
| Uses: | Can be used on ELISA, Immunofluorescence and Western blotting. A band at ~57 kDa can be seen in Western blotting. |
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| Dilutions: | ELISA, Immunocytochemistry, Immunofluorescence 1:1000, Western Blot 1:5000 |
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| Unit Size: | 0.1 ml |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Peripherin is a Class III intermediate filament subunit found in both the peripheral and central nervous systems, though it is concentrated, as its name suggests, in the neurons of peripheral ganglia and their processes. Antibodies to peripherin can be used in identifying, classifying, and studying neurons in the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease and some neuronally derived tumors. Peripherin is also a major component of the cytoskeleton of PC12 cells, widely used model system for studies of neuronal differentiation. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.
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Images (1) Reviews (1) Related Products (22) Exploring Peripherin Working with Peripherin
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with Peripherin
Peripherin Antibody Images (1) Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule. |
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Reviewed by:
Anonymous 8/17/2010
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Application:
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IHC
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Sample Tested:
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spinal cord
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Species:
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Mouse
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Sample Preparation:
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OCT Embedded Cryosection
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Sample Pretreatment:
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none
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Results:
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worked as expected in IHC after RNA in situ hybridization
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Blocking Buffer:
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0.2% casein
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Blocking Time:
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60min
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Blocking Temperature:
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4 Celsius
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Dilution Ratio:
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1:500
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Incubation Time:
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overnight
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Incubation Temperature:
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4 4
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Incubation Dilution Buffer:
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0.2% casein TBS tween 20%
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Description:
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Alexa 488
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Dilution Ratio:
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1:400
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Visualization Method:
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confocal
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Did you use controls?:
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No
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Publications (3)Peripherin Antibody Product Specific:- Yellon SM, Burns AE, See JL, et al. Progesterone Withdrawal Promotes Remodeling Processes in the Nonpregnant Mouse Cervix. Biol Reprod 2009;81(1):1-6. [pubmed: 19228593]
- Yellon SM, Burns AE, See JL, et al. Progesterone Withdrawal Promotes Remodeling Processes in the Nonpregnant Mouse Cervix. Biol Reprod. 2009 Feb 18. [pubmed: 19228593]Burgess, R. W., Jucius, T. J., Ackerman, S. L. (2006) Motor Axon Guidance of the Mammalian Trochlear Phrenic Nerves: Dependence on the Netrin Receptor Unc5c Modifier Loci, 26, 5756-5766.
more... - Arvanian, V., et al. Viral Delivery of NR2D Subunits Reduces Mg(2+) Block of NMDA Receptor Restores NT-3-Induced Potentiation of AMPA-Kainate Responses in Maturing Rat Motoneurons. J. Neurophysiol. 92: 2394-2404, 2004.
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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsLysates (2)Peptides and ProteinsPrimary Antibodies (17)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Peripherin Antibody (8G2) | NB300-138 | Hu, Ma | ELISA, ICC, IHC-P, WB | | (1) | | | | Peripherin Antibody (7C5) | NB300-220 | Ch, Ma, Rt | ICC, WB | (1) | | | | | Peripherin Antibody | NBP1-05423 | Rt | IF, WB | (1) | (1) | | | | Peripherin Antibody | NBP1-67667 | Hu, Rt | ELISA, IHC, WB | (2) | | | | | Peripherin Antibody | PAB12115 | Hu, Rt | ELISA, ICC, IF, IHC-P, WB | | | | | | ... and 12 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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