PSMC5 Antibody (NB100-345)
PSMC5 Antibody (25D5)
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Distributor Data...
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anti-TRIP1 antibody, anti-TRIP-1 antibody, anti-Thyroid Receptor Interactor 1 antibody, anti-TBP10 antibody, anti-Tat-binding protein homolog 10 antibody, anti-SUG1 antibody, anti-S8 antibody, anti-PSMC5 antibody, anti-proteasome (prosome Antibody, anti-p45/SUG antibody, anti-p45 antibody, anti-macropain) 26S subunit Antibody, anti-ATPase antibody, anti-5 antibody |
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PSMC5 Antibody (25D5) Summary: | | Gene: | PSMC5 |
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| Purity: | Protein G purified |
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| Host: | Mouse |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional PSMC5 Products PSMC5 Antibody (25D5) Details: | Clone: | 25D5 |
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| Isotype: | IgG |
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| Immunogen: | Recombinant SUG1 purified from E. coli. |
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| Localization: | Cytoplasmic |
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| Uses: | This antibody is useful for Western Blot. ChIP – See customer review. |
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| Dilutions: | Western Blot 1:1000 |
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| TMW: | 45 kDa |
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| Unit Size: | 0.1 mg |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | PBS, pH7.4 |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.
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Images (1) Reviews (1) Related Products (9) Working with PSMC5
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with PSMC5
PSMC5 Antibody (25D5) Images (1)-Western-Blot-NB100-345-img0001.jpg "Western Blot: PSMC5 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji") Western Blot: PSMC5 Antibody (25D5) [NB100-345] - SUG1 antibody with 30 ug whole cell lysate run on SDS-PAGE. Lane A: 293T; B: A431; C: H1299; D: Hela; E: Hep G2; F: Molt-4; G: Raji |
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Reviewed by:
Anonymous 3/2/2010
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Application:
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ChIP
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Sample Tested:
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Human Whole Cell lysate
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Species:
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Human
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Positive Control:
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IgG binding
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ChIP Type:
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Cross-linking (X-ChIP)
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Results:
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It works well with both ChIP and WB. I am happy wit the Ab.
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Dilution Ratio:
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Undiluted
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Incubation Time:
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overnight
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Incubation Temperature:
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4 4
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Incubation Dilution Buffer:
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ChIP dilution Biffer
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Publications (3)PSMC5 Antibody (25D5) Background References:- Lommel, L., et al. The 26S proteasome negatively regulates the level of overall genomic nucleotide excision repair. Nucl. Acid Res. 24:4839, 2000.
- Schauber, C., et al. Rad23 links DNA repair to the ubiquitin/proteasome pathway. Nature. 39:715, 1998.
more... - Weeda, G., et al. The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteosome putative transcription factor. Nucl. Acid Res. 25:2274, 1997.
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Ask a Scientist
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsLysates (1)Peptides and ProteinsPrimary Antibodies (5)RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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