PPAR alpha Antibody (H00005465-B01)

PPAR alpha Antibody

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PPAR alpha Antibody Summary:
Species:Hu
Applications:ELISA, IF, WB
Clonality:Polyclonal
Gene:PPARA
Purity:Whole antisera
Host:Mouse
Specificity:PPARA - peroxisome proliferative activated receptor, alpha,
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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PPAR alpha Antibody Details:
Immunogen:PPARA (NP_001001928, 1 a.a. ~ 468 a.a) full-length human protein.
Species Reactivity:

Human. Other species not tested.

Applications:
Uses:Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for immunofluorescence.
Dilutions:ELISA, immunofluorescence, Western Blot 1:500,
Unit Size:0.05 ml
Concentration:This product is unpurified. Concentration is not relevant.
Positive Controls:1 Positive Controls
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:This is unpurified antiserum so it is not possible to supply the specific antibody concentration.
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: PPARA
Entrez5465 (Human)
Swiss ProtNP_001001928 (Human)
 
Background:

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.

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Working with PPAR alpha

PPAR alpha Antibody Images (2)
Western Blot analysis of PPARA expression in transfected 293T cell line by PPARA MaxPab polyclonal antibody (B01).

Lane 1: PPARA transfected lysate(52.20 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to PPARA on HepG2 cell. [antibody concentration 20 ug/ml]
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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PPAR alpha in Context


Research Areas related to PPAR alpha (3):
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