Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide with sequence C-NAIIKNAYKKGE, representing the C Terminus of the human protein according to NP_000930.1; NP_001030333.1
Epitope:
C Terminus
Species Reactivity:
Human, Mouse and Rat.
Applications:
Uses:
This antibody is useful for Peptide ELISA, Western Blot and Immunohistochemistry. Western Blot: Approx. 26kDa band observed in lysates of Mouse pituitary cell line NIH3T3 (calculated MW of 29.4kDa according to NP_000930.1 and NP_001030333.1). Immunohistochemistry: In paraffin embedded Human Adrenal Gland shows strong cytoplasm staining in select cells in the medulla.
Dilutions:
Peptide ELISA 1:2000, Western Blot 1:1000, Immunohistochemistry 1:200
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
Tris saline [pH7.3] containing 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Muhlhausler BS, Adam CL, Marrocco E, Findlay PA, Roberts CT, McFarlane JR, Kauter KG, McMillen IC. Impact of glucose infusion on the structural functional characteristics of adipose tissue on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005 Jan 20; [Epub ahead of print]