PINK1 Antibody 0.025 ml

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Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: PINK1 Antibody [NBP1-49678] - Stain in paraffin embedded mouse brain.
Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: PINK1 Antibody [NBP1-49678] - Simple Western lane view shows a specific band for PINK1 in 1.0 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
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Ordering Information: NBP1-49678

  • Catalog Number
    NBP1-49678
  • Displayed Format
    Unconjugated
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PINK1 Antibody Summary

Species Human, Mouse
Tested Applications Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
PINK1
Purity
Immunogen affinity purified
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PINK1 Antibody Details

Immunogen
A genomic peptide made to an internal region of the human PINK1 protein (within residues 350-500). [Swiss-Prot Q9BXM7]
Localization
Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm - cytosol

Species Reactivity

Human and mouse.

Applications/Dilutions

Dilutions
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This PINK1 antibody is useful for IHC and ICC/IF. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-49678AF405
Alexa Fluor (R) 488 Labeled NBP1-49678AF488
Alexa Fluor (R) 647 Labeled NBP1-49678AF647
Alexa Fluor (R) 700 Labeled NBP1-49678AF700
Biotin Labeled NBP1-49678B
DyLight 405 Labeled NBP1-49678V
DyLight 488 Labeled NBP1-49678G
DyLight 550 Labeled NBP1-49678R
DyLight 650 Labeled NBP1-49678C
HRP Labeled NBP1-49678H

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Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Unit Size
0.025 ml
Concentration
1.2 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol PINK1
Entrez
Uniprot

Alternate Names for PINK1 Antibody

  • BRPK
  • EC 2.7.11.1
  • FLJ27236
  • PARK6
  • Parkinson disease (autosomal recessive) 6
  • protein kinase BRPK
  • PTEN induced putative kinase 1
  • PTEN-induced putative kinase protein 1
  • serine/threonine-protein kinase PINK1, mitochondrial
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Related Products by Gene

Background

PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolytic processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.

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Product General Protocols

View specific protocols for PINK1 Antibody (NBP1-49678): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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FAQs for PINK1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Bioinformatics Tool for PINK1

Discover related pathways, diseases and genes to PINK1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

PTMs for PINK1

Learn more about PTMs related to PINK1.

Blogs on PINK1

Check out the latest blog posts on PINK1.
PINK1 and its role in Parkinson's disease
PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondr...  Read full blog post.

PINK1: Promoting Organelle Stability and Preventing Parkinson's disease
PINK1 is a protein serine/threonine kinase (PTK) that protects the organelles from cellular stress and controls selective autophagy to clear damage. Exner, et al. were among the first to report that PINK1 deficiency in humans was linked to autosomal r...  Read full blog post.

PINK1: Promoting Organelle Stability and Preventing Parkinson's disease
PINK1 is a protein serine/threonine kinase (PTK) that protects the organelles from cellular stress and controls selective autophagy to clear damage. Exner, et. al. were among the first to report that PINK1 deficiency in humans was linked to autosomal ...  Read full blog post.

PINK1: Linking Mitochondrial Health and Parkinson's disease
Parkinson's disease is a degenerative disorder of the central nervous system, which involves the loss of dopaminergic neurons in the brain and gives rise to tremors, rigidity and slowness of movement. In the majority of cases there is no known cause; ...  Read full blog post.

PINK1: A Critical Player in Mitophagy
PINK1 (PTEN-induced putative kinase 1) is a mitochondrial directed serine-threonine kinase, that regulates normal mitochondrial function and transport vital to normal performance of neurons and neuronal survival. PINK1 has been shown to be localized t...  Read full blog post.